Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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First Report of Echinococcus equinus in a Donkey in Turkey

  • Simsek, Sami (Department of Parasitology, Faculty of Veterinary Medicine, University of Firat) ;
  • Roinioti, Erifylli (Department of Anatomy and Physiology of Farm Animals, Agricultural University of Athens) ;
  • Eroksuz, Hatice (Department of Pathology, Faculty of Veterinary Medicine, University of Firat)
  • Received : 2015.05.31
  • Accepted : 2015.09.09
  • Published : 2015.12.31
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Abstract

A 2-year-old female donkey (Equus asinus) was euthanized in the Pathology Department of Firat University, Elazig, Turkey. Necropsy disclosed the presence of 7 hydatid cysts distributed throughout the lung parenchyma. One of those cysts represented the parasite material of the present study and was molecularly identified through sequencing of a fragment of cytochrome c oxidase subunit 1 (CO1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (NADH1) gene, as Echinococcus equinus. The generated CO1 sequence supports the presence of the dominant haplotype as has been described in Europe and Africa. The NADH1 sequence was found similar to sequences reported in equids in Egypt and the United Kingdom. The molecular identification of E. equinus in a donkey is being reported for the first time in Turkey.

Keywords

References

  1. Nakao M, Yanagida T, Konyaev S, Lavikainen A, Odnokurtsev VA, Zaikov VA, Ito A. Mitochondrial phylogeny of the genus Echinococcus (Cestoda: Taeniidae) with emphasis on relationships among Echinococcus canadensis genotypes. Parasitology 2013; 140: 1625-1636. https://doi.org/10.1017/S0031182013000565
  2. Jenkins DJ, Romig T, Thompson RCA. Emergence/re-emergence of Echinococcus spp.-a global update. Int J Parasitol 2005; 35: 1205-1219. https://doi.org/10.1016/j.ijpara.2005.07.014
  3. Boufana B, Lett WS, Lahmar S, Buishi I, Bodell AJ, Varcasia A, Casulli A, Beeching NJ, Campbell F, Terlizzo M, McManus DP, Craig PS. Echinococcus equinus and Echinococcus granulosus sensu stricto from the United Kingdom: genetic diversity and haplotypic variation. Int J Parasitol 2014; 45: 161-166.
  4. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 1992; 54: 165-174. https://doi.org/10.1016/0166-6851(92)90109-W
  5. Bowles J, McManus DP. NADH dehydrogenase 1 sequences compared for species and strains of the genus Echinococcus. Int J Parasitol 1993; 23: 969-972. https://doi.org/10.1016/0020-7519(93)90065-7
  6. Varcasia A, Garippa G, Pipia AP, Scala A, Brianti E, Giannetto S, Battelli G, Poglayen G, Micagni G. Cystic echinococcosis in equids in Italy. Parasitol Res 2008; 102: 815-818. https://doi.org/10.1007/s00436-007-0862-7
  7. Gonzalez LM, Mwambete KD, Montero E, Rosenzvit MC, McManus DP, Garate T, Bandera CC. Further molecular discrimination of Spanish strains of Echinococcus granulosus. Exp Parasitol 2002; 102: 46-56. https://doi.org/10.1016/S0014-4894(02)00146-7
  8. Blutke A, Hamel D, Huttner M, Gehlen H, Romig T, Pfister K, Hermanns W. Cystic echinococcosis due to Echinococcus equinus in a horse from Southern Germany. J Vet Diagn Invest 2010; 22: 458-462. https://doi.org/10.1177/104063871002200323
  9. Kumaratilake LM, Thompson RCA, Eckert J. Echinococcus granulosus of equine origin from different countries possess uniform morphological characteristics. Int J Parasitol 1986; 16: 529-540. https://doi.org/10.1016/0020-7519(86)90089-5
  10. Aboelhadid SM, El-Dakhly KM, Yanai T, Fukushi H, Hassanin KM. Molecular characterization of Echinococcus granulosus in Egyptian donkeys. Vet Parasitol 2013; 193: 292-296. https://doi.org/10.1016/j.vetpar.2012.11.019
  11. Boufana B, Lahmar S, Rebai W, Ben Safta Z, Jebabli L, Ammar A, Kachti M, Aouadi S, Craig PS. Genetic variability and haplotypes of Echinococcus isolates from Tunisia. Trans R Soc Trop Med Hyg 2014; 108: 706-714. https://doi.org/10.1093/trstmh/tru138
  12. Simsek S, Cevik A. First detection and molecular characterization of Echinococcus equinus in a mule in Turkey. Acta Parasitologica 2014; 59: 773-777.
  13. Boufana B, Stidworthy MF, Bell S, Chantrey J, Masters N, Unwin S, Wood R, Lawrence RP, Potter A, McGarry J, Redrobe S, Killick R, Foster AP, Mitchell S, Greenwood AG, Sako Y, Nakao M, Ito A, Wyatt K, Lord B, Craig PS. Echinococcus and Taenia spp. from captive mammals in the United Kingdom. Vet Parasitol 2012; 190: 95-103. https://doi.org/10.1016/j.vetpar.2012.05.023
  14. Wassermann M, Aschenborn O, Aschenborn J, Mackenstedt U, Romig T. A sylvatic lifecycle of Echinococcus equinus in the Etosha National Park, Namibia. Int J Parasitol: Parasites and Wildlife 2014; doi: 10.1016/j.ijppaw.2014.12.002.
  15. Dinkel A, Njoroge EM, Zimmermann A, Walz M, Zeyhle E, Elmahdi IE, Mackenstedt U, Romig T. A PCR system for detection of species and genotypes of the Echinococcus granulosus complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol 2004; 34: 645-653. https://doi.org/10.1016/j.ijpara.2003.12.013
  16. Altschul SF, Madden TL, Schaffer AA., Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSL-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25: 3389-3402. https://doi.org/10.1093/nar/25.17.3389
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406-425.
  18. Knudsen B, Knudsen T, Flensborg M, Sandmann H, Heltzen M, Andersen A, Dickenson M, Bardram J, Steffensen PJ, Monsted S, Lauritzen T, Forsberg R, Thanbichler A, Bendtsen JD, Gorlitz L, Rasmussen J, Tordrup D, Vaerum M, Ravn MN, Hachenberg C, Fisker E, Dekker P, Schultz J, Hein AMK, Sinding JB. CLC Main Workbench. Version 5.5. Aarhus, Denmark, CLC Bio. 2007.
  19. Romig T, Dinkel A, Mackenstedt U. The present situation of echinococcosis in Europe. Parasitol Int 2006; 55 (suppl): S187-S191. https://doi.org/10.1016/j.parint.2005.11.028
  20. Ziadinov I, Mathis A, Trachsel D, Rysmukhambetova A, Abdyjaparov TA, Kuttubaev OT, Deplazes P, Torgerson PR. Canine echinococcosis in Kyrgyzstan: using prevalence data adjusted for measurement error to develop transmission dynamics models. Int J Parasitol 2008; 38: 1179-1190. https://doi.org/10.1016/j.ijpara.2008.01.009
  21. Al-Qaoud KM, Abdel-Hafez SK, Craig PS. Canine echinococcosis in northern Jordan: increased prevalence and dominance of sheep/dog strain. Parasitol Res 2003; 90: 187-191. https://doi.org/10.1007/s00436-002-0793-2

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