Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea

  • Jeon, Hyeong-Kyu (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Park, Hansol (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Lee, Dongmin (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Choe, Seongjun (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine) ;
  • Kim, Kyu-Heon (Ministry of Food and Drug Safety) ;
  • Huh, Sun (Department of Parasitology, College of Medicine, Hallym University) ;
  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Eom, Keeseon S. (Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine)
  • Received : 2015.03.10
  • Accepted : 2015.05.18
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Tapeworms of the genus Spirometra are pseudophyllidean cestodes endemic in Korea. At present, it is unclear which Spirometra species are responsible for causing human infections, and little information is available on the epidemiological profiles of Spirometra species infecting humans in Korea. Between 1979 and 2009, a total of 50 spargana from human patients and 2 adult specimens obtained from experimentally infected carnivorous animals were analyzed according to genetic and taxonomic criteria and classified as Spirometra erinaceieuropaei or Spirometra decipiens depending on the morphology. Morphologically, S. erinaceieuropaei and S. decipiens are different in that the spirally coiled uterus in S. erinaceieuropaei has 5-7 complete coils, while in S. decipiens it has only 4.5 coils. In addition, there is a 9.3% (146/1,566) sequence different between S. erinaceieuropaei and S. decipiens in the cox1 gene. Partial cox1 sequences (390 bp) from 35 Korean isolates showed 99.4% (388/390) similarity with the reference sequence of S. erinaceieuropaei from Korea (G1724; GenBank KJ599680) and an additional 15 Korean isolates revealed 99.2% (387/390) similarity with the reference sequences of S. decipiens from Korea (G1657; GenBank KJ599679). Based on morphologic and molecular databases, the estimated population ratio of S. erinaceieuropaei to S. decipiens was 35: 15. Our results indicate that both S. erinaceieuropaei and S. decipiens found in Korea infect humans, with S. erinaceieuropaei being 2 times more prevalent than S. decipiens. This study is the first to report human sparganosis caused by S. decipiens in humans in Korea.

Keywords

References

  1. Mueller JF. A repartition of the genus Diphyllobothrium. J Parasitol 1937; 23: 308-310. https://doi.org/10.2307/3272427
  2. Faust EC, Campbell HE, Kellogg CR. Morphological and biological studies on the species of Diphyllobothrium in China. Am J Hyg 1929; 9: 560-583.
  3. Wardle RA, McLeod JA. The Zoology of Tapeworms. Minneapolis, USA. University of Minnesota Press. 1952, pp 559-615.
  4. Yamaguti S. Systema Helminthum. Vol. II. The cestodes of vertebrates. New York, USA. Interscience Publishers. 1959, pp 358-361.
  5. Iwata S. Experimental and morphological studies of Manson's tapeworm, Diphyllobothrium erinacei, Rudolphi. Special reference with its scientific name and relationship with Sparganum proliferum, Ijima. Progr Med Parasitol Jpn 1972; 4: 536-590.
  6. Mueller JF. The biology of Spirometra. J Parasitol 1974; 60: 3-14.
  7. Lee SH, We SJ, Sohn WM, Hong ST, Chai JY. Experimental life history of Spirometra erinacei. Korean J Parasitol 1990; 28: 161-173. https://doi.org/10.3347/kjp.1990.28.3.161
  8. Kobayashi H. On the animal parasites in Chosen (Korea). Second report. Acta Med Keijo 1928; 11: 109-124.
  9. Cho SY, Bae JH, Seo BS. Some aspects of human sparganosis in Korea. Korean J Parasitol 1975; 13: 60-77. https://doi.org/10.3347/kjp.1975.13.1.60
  10. Min DY. Cestode infections in Korea. Korean J Parasitol 1990; 28 (suppl): 123-44. https://doi.org/10.3347/kjp.1990.28.Suppl.123
  11. Lee SH, Chai JY, Seo BS, Cho SY. Two cases of human infection by adult of Spirometra erinacei. Korean J Parasitol 1984; 22: 71-77.
  12. Jeon HK, Kim KH, Eom KS. Molecular identification of Taenia specimens after long-term preservation in formalin. Parasitol Int 2011; 60: 203-205. https://doi.org/10.1016/j.parint.2010.12.001
  13. Swofford DL. Paup*: phylogenetic analysis using parsimony (* and other methods). Version 4. Sunderland, Massachusetts, USA. Sinauer Associates. 2003.
  14. Bearup AJ. Observations on the life cycle of Diphyllobothrium (Spirometra) erinacei in Australia (Cestoda: Diphyllobothriidae). Aust J Sci 1948; 10: 183.
  15. Yamane Y, Okada N, Takihara M. On a case of long term migration of Spirometra erinacei larva in the breast of a woman. Yonago Acta Med 1975; 19: 207-213.
  16. Schmidt GD. CRC Handbook of Tapeworm Identification. 2nd Printing. Boca Raton, Florida, USA. CRC Press, Inc. 1986, pp. 93-95.

Cited by

  1. Mitochondrial Genome Sequences of Spirometra erinaceieuropaei and S. decipiens (Cestoidea: Diphyllobothriidae) vol.53, pp.4, 2015, https://doi.org/10.3347/kjp.2015.53.4.455
  2. Rapid identification of nine species of diphyllobothriidean tapeworms by pyrosequencing vol.6, pp.None, 2016, https://doi.org/10.1038/srep37228
  3. Genetic Identification of Spirometra decipiens Plerocercoids in Terrestrial Snakes from Korea and China vol.54, pp.2, 2015, https://doi.org/10.3347/kjp.2016.54.2.181
  4. Molecular Detection of Spirometra decipiens in the United States vol.54, pp.4, 2015, https://doi.org/10.3347/kjp.2016.54.4.503
  5. Molecular Identification of Spirometra erinaceieuropaei Tapeworm in Cases of Human Sparganosis, Hong Kong vol.23, pp.4, 2015, https://doi.org/10.3201/eid2304.160791
  6. Case report: the first case of human infection by adult of SPIROMETRA ERINACEIEUROPAEI in VIETNAM vol.17, pp.None, 2017, https://doi.org/10.1186/s12879-017-2786-x
  7. Spirometra decipiens (Cestoda: Diphyllobothriidae) Collected in A Heavily Infected Stray Cat from the Republic of Korea vol.56, pp.1, 2015, https://doi.org/10.3347/kjp.2018.56.1.87
  8. Genetic and Morphologic Identification of Spirometra ranarum in Myanmar vol.56, pp.3, 2018, https://doi.org/10.3347/kjp.2018.56.3.275
  9. Differential Diagnosis of Human Sparganosis Using Multiplex PCR vol.56, pp.3, 2015, https://doi.org/10.3347/kjp.2018.56.3.295
  10. Molecular Genetic Findings of Spirometra decipiens and S. ranarum in Korea vol.56, pp.4, 2015, https://doi.org/10.3347/kjp.2018.56.4.359
  11. Molecular and Morphologic Identification of Spirometra ranarum Found in the Stool of African Lion, Panthera leo in the Serengeti Plain of Tanzania vol.56, pp.4, 2015, https://doi.org/10.3347/kjp.2018.56.4.379
  12. Human Sparganosis in Korea vol.33, pp.44, 2015, https://doi.org/10.3346/jkms.2018.33.e273
  13. Complete Sequence of the Mitochondrial Genome of Spirometra ranarum: Comparison with S. erinaceieuropaei and S. decipiens vol.57, pp.1, 2015, https://doi.org/10.3347/kjp.2019.57.1.55
  14. The medical relevance of Spirometra tapeworm infection in Indonesian Bronzeback snakes (Dendrelaphis pictus): A neglected zoonotic disease vol.12, pp.6, 2015, https://doi.org/10.14202/vetworld.2019.844-848
  15. Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China vol.57, pp.3, 2019, https://doi.org/10.3347/kjp.2019.57.3.309
  16. Characterization of the complete mitochondrial genome of Spirometra decipiens (Cestoda: Diphyllobothriidae) from China vol.4, pp.2, 2019, https://doi.org/10.1080/23802359.2019.1659117
  17. Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries vol.57, pp.5, 2019, https://doi.org/10.3347/kjp.2019.57.5.481
  18. Large-scale survey of a neglected agent of sparganosis Spirometra erinaceieuropaei  (Cestoda: Diphyllobothriidae) in wild frogs in China vol.14, pp.2, 2020, https://doi.org/10.1371/journal.pntd.0008019
  19. Ocular Sparganosis: The First Report of Spirometra ranarum in Thailand vol.58, pp.5, 2015, https://doi.org/10.3347/kjp.2020.58.5.577
  20. Morphological and Molecular Identification of Spirometra Tapeworms (Cestoda: Diphyllobothriidae) from Carnivorous Mammals in the Serengeti and Selous Ecosystems of Tanzania vol.58, pp.6, 2015, https://doi.org/10.3347/kjp.2020.58.6.653
  21. A Case of Sparganosis Caused by Spirometra decipiens on the Femur vol.83, pp.5, 2021, https://doi.org/10.2336/nishinihonhifu.83.453
  22. Insight into One Health Approach: Endoparasite Infections in Captive Wildlife in Bangladesh vol.10, pp.2, 2015, https://doi.org/10.3390/pathogens10020250
  23. Sparganosis (Spirometra) in Europe in the Molecular Era vol.72, pp.5, 2015, https://doi.org/10.1093/cid/ciaa1036
  24. An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae vol.1069, pp.None, 2015, https://doi.org/10.3897/zookeys.1069.67403
  25. Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid vol.59, pp.6, 2021, https://doi.org/10.3347/kjp.2021.59.6.615