Parasites, Hosts and Diseases

  • 제53권3호
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  • Pages.271-277
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  • 2015
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Genetic Diversity of Schistosoma haematobium Eggs Isolated from Human Urine in Sudan

  • Quan, Juan-Hua (Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College) ;
  • Choi, In-Wook (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Ismail, Hassan Ahmed Hassan Ahmed (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Mohamed, Abdoelohab Saed (National Control Program for Neglected Tropical Diseases, Federal Ministry of Health) ;
  • Jeong, Hoo-Gn (Korea Association of Health Promotion (KAHP)) ;
  • Lee, Jin-Su (Korea Association of Health Promotion (KAHP)) ;
  • Hong, Sung-Tae (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Yong, Tai-Soon (Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Cha, Guang-Ho (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Lee, Young-Ha (Department of Infection Biology, Chungnam National University School of Medicine)
  • 투고 : 2014.06.19
  • 심사 : 2015.04.15
  • 발행 : 2015.06.30
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초록

The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.

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참고문헌

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피인용 문헌

  1. Diagnostic Value of SjR2 Gene in Colonic Tissue from Schistosoma Japonicum Infected Hosts vol.25, pp.None, 2015, https://doi.org/10.12659/msm.912997