한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제30권3호
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  • Pages.121-128
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  • 2015
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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항산화 효소가 첨가된 Percoll에 의해 분리한 돼지 정액의 동결-융해 능력

Cryo-Ability of Boar Sperm sorted by Percoll Containing of Antioxidative Enzyme

  • 이경진 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 주선호 (강원대학교 동물생명과학대학) ;
  • 김유진 (강원대학교 동물생명과학대학) ;
  • 양진우 (강원대학교 동물생명과학대학) ;
  • 이연주 (강원대학교 동물생명과학대학) ;
  • 황보용 (강원대학교 동물생명과학대학) ;
  • 이승형 (강원대학교 동물생명과학대학) ;
  • 이승태 (강원대학교 동물생명과학대학) ;
  • 이은송 (강원대학교 수의과대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Lee, Kyung-Jin (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Joo, Seon-Ho (College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Yu-Jin (College of Animal Life Sciences, Kangwon National University) ;
  • Yang, Jin-Woo (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Yeon-Ju (College of Animal Life Sciences, Kangwon National University) ;
  • Hwangbo, Yong (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seung Tae (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Eunsong (College of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 투고 : 2015.08.13
  • 심사 : 2015.09.23
  • 발행 : 2015.09.30
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⟨ 이전 논문 다음 논문 ⟩

초록

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed ($37^{\circ}C$) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at $38.5^{\circ}C$ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

키워드

참고문헌

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