한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제30권2호
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  • Pages.83-89
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  • 2015
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
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Sexually Matured Female Mice showed Higher γ-Linolenic Acid (GLA) in the Muscle Tissue

  • Park, Chang Seok (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Park, Young Sik (Department of Animal Science and Biotechnology, Kyungpook National University)
  • 투고 : 2015.04.21
  • 심사 : 2015.06.14
  • 발행 : 2015.06.30
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초록

The sexual maturation occurred by the changes of steroid hormones was known to sex-dependently and/or age-dependently regulate the lipid metabolism in various animal species. Our current study demonstrates that lipid and its functional fatty acids can be changed depending on the status of sexual maturation. Of the functional fatty acids, ${\gamma}$-linolenic acid (GLA; 18:3n-6) is an important factor for maintaining human health. The purpose of our study was to investigate the level of GLA in mice with different stages of sexual maturation. To this end, the longissimus muscle (LM) of immature (3-week-old) and mature (7-week-old) female mice was analysed for the fatty acid composition by gas chromatography. Furthermore, both gene and protein level of ${\Delta}6$ desaturase (FADS2) which is involved in GLA metabolism by real time PCR and Western blotting, respectively. Mature females showed greater (P<0.05) serum $17{\beta}$-estradiol (E2) level and LM GLA contents than immature group. The mRNA and protein levels of FADS2, which converts precursor linoleic acid into GLA, were higher (P<0.05) in mature female mice than in immature mice. In conclusion, these results show that sexual maturation of female mice induces GLA and FADS2 contents in LM.

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참고문헌

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