Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Effect of Cytosine Arabinoside Pre-treatment in Dorsal Root Ganglion Explant Culture

Cytosine Arabinoside 전처리가 척수후근절 외식편 배양에 미치는 영향

  • Jung, H.Y. (Department of Biomedical Engineering, Korea Institute of Science and Technology) ;
  • Han, S. (Department of Biomedical Engineering, Korea Institute of Science and Technology) ;
  • Park, J.W. (Department of Biomedical Sciences, Korea University College of Medicine) ;
  • Youn, I. (Department of Biomedical Engineering, Korea Institute of Science and Technology)
  • 정호영 (한국과학기술연구원 의공학연구소) ;
  • 한성민 (한국과학기술연구원 의공학연구소) ;
  • 박종웅 (고려대학교 의과대학 의과학과) ;
  • 윤인찬 (한국과학기술연구원 의공학연구소)
  • Received : 2015.12.11
  • Accepted : 2015.12.19
  • Published : 2015.12.31
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Abstract

Explant culture condition of dorsal root ganglion have been used to investigate the pathophysiology of peripheral nerve injury, while applying for the various clinical symptom such as trauma, pressure, and stretch. However, explant culture is usually contaminated by mitotic cells, which may observed as a newly divided cells including fibroblast or glia. The mitotic cells could be able to interrupt and change the cell signaling that make it difficult to avoid detrimental effects during the experiments. To eliminate mitotic cells, anti-mitotic reagents like mixture of uridine and 5-fluorodeoxyuridine or cytosine arabinoside were added to the cultures on the following day, but there is no research that investigate viability of anti-mitotic reagent in dorsal root ganglion explant culture. In this study, we investigate inhibition effect of cytosine arabinoside to mitotic cells in dorsal root ganglion explant culture. Also we visualized and analyzed anti-mitotic effect and toxicity of cytosine arabinoside in various concentration condition. This dorsal root ganglion explant culture condition can be applied to research that effect and mechanism of various stimulation and chemical application which affect peripheral nerve regeneration.

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References

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