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Pretreatment and Enzymatic Saccharification of Wasted MDF for Bioethanol Production

바이오에탄올 생산을 위한 폐MDF의 전처리 및 효소 당화

  • Kang, Yang-Rae (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.) ;
  • Hwang, Jin-Sik (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.) ;
  • Bae, Ki-Han (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.) ;
  • Cho, Hoon-Ho (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.) ;
  • Lee, Eun-Jeong (Department of Plant Resources, Gyeongnam National University of Science & Technology) ;
  • Cho, Young-Son (Department of Plant Resources, Gyeongnam National University of Science & Technology) ;
  • Nam, Ki-Du (Il San Institute, Chilseo Ethanol Factory, Il San Trading Co., Ltd.)
  • 강양래 (일산실업(주) 칠서에탄올공장 부설기술연구소) ;
  • 황진식 (일산실업(주) 칠서에탄올공장 부설기술연구소) ;
  • 배기한 (일산실업(주) 칠서에탄올공장 부설기술연구소) ;
  • 조훈호 (일산실업(주) 칠서에탄올공장 부설기술연구소) ;
  • 이은정 (경남과학기술대학교 식물자원학과) ;
  • 조영손 (경남과학기술대학교 식물자원학과) ;
  • 남기두 (일산실업(주) 칠서에탄올공장 부설기술연구소)
  • Received : 2015.11.05
  • Accepted : 2015.12.22
  • Published : 2015.12.27

Abstract

The objective of this study was designed to determine the possibility of bioethanol production from wasted medium density fiberboard (wMDF). We were investigated the enzymatic saccharification characteristics using the enzyme (Cellic CTec3) after pretreatment with sodium chlorite. According to the component analysis results, the lignin contents before and after the pretreatment of wMDF (milling using sieve size of $1,000{\mu}m$) was significantly reduced from 31.13% to 4.11%. Therefore, delignification ratio of pretreated wMDF was found to be up to about 87-89% depending on the sieve size. And we were tested to compare the saccharification ratio according to the sieve size of wMDF ($1,000{\mu}m$, $200{\mu}m$), but it was no significance depending on the sieve size. When enzyme dosage was 5% based on the substrate concentration, enzymatic saccharification ratio was obtained up to 70% by maintaining at $50^{\circ}C$ for 72 hours. We could made the substrate concentration of pretreated wMDF ($1,000{\mu}m$) up to 12% and then enzymatic saccharification ratio was 76.8%, also contents of glucose and xylose were analyzed to 77,750 and 14,637 mg/L, respectively.

Keywords

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