Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Isolation of Antimicrobial Active Substance from Usnea longissima against Sclerotial Rot (Sclerotinia sclerotiorum)

송라(Usnea longissima)추출물로부터 균핵병 병원균(Sclerotinia sclerotiorum)에 대한 항균 활성물질 탐색

  • 권유빈 (경북대학교 대학원 생태환경시스템학과) ;
  • 최용화 (경북대학교 생태환경대학 생태환경시스템학부)
  • Received : 2015.11.06
  • Accepted : 2015.11.16
  • Published : 2015.12.31
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Abstract

To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Usnea longissima was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by silica gel-column chromatography and obtained into nine group subfractions. The nine group fractions were searched the antifungal activities by bioassay. The most active No. 3 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to database of Wiley library. As a result, Usnic acid was identified as main compounds. In conclusion, Usnic acid isolated from Usnea longissima was antimicrobial chemical against Sclerotinia sclerotiorum as a pathogen of sclerotium disease.

본 연구는 균핵병의 원인균인 Sclerotinia sclerotiorum에 대해 항균활성을 갖는 친환경 유기농자재를 개발할 목적으로 송라(Usnea longissima)로부터 활성물질을 분리하였다. 송라를 MeOH로 추출하여 용매분획을 하였고, 용매분획 중에서 강한 활성을 나타낸 $CHCl_3$ fraction을 column chromatography로 분리하여 40개의 subfractions을 얻었고 9개의 그룹으로 나누었다. 9개의 subfractions을 bioassay한 결과 G3 subfraction에서 강한 활성을 나타내었다. G3 subfraction을 GC-MS로 분석하여 chromatogram 상의 활성물질로 추정되는 peak의 mass spectrum과 Wiley library를 비교하여 profiling한 결과, Usnic acid가 주요 물질로 검출되었다. 항균활성물질로 추정되는 Usnic acid 화합물의 항균활성을 검정하기 위하여 표준품을 사용하여 생물검정한 결과 이 화합물이 강한 활성을 나타내었다. 따라서 송라로부터 분리한 Usnic acid가 균핵병의 원인균인 Sclerotinia sclerotiorum에 대한 항균 활성물질인 것으로 추정하였다.

Keywords

References

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