Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Emergence of Acinetobacter pittii Harboring New Delhi Metallo-β-Lactamase Genes in Daejeon, Korea

  • Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University) ;
  • Koo, Sun Hoe (Department of Laboratory Medicine, College of Medicine, Chungnam National University) ;
  • Kim, Semi (Department of Laboratory Medicine, College of Medicine, Chungnam National University) ;
  • Kwon, Gye Cheol (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
  • Received : 2015.01.23
  • Accepted : 2015.06.23
  • Published : 2015.09.01
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Abstract

Carbapenemase production has been reported worldwide in gram-negative bacteria, including Acinetobacter species. We detected carbapenemase-producing Acinetobacter pittii in clinical isolates in Daejeon, Korea. Twenty-one ertapenem-resistant A. pittii isolates screened with a disk diffusion method were characterized by using the Epsilon test, four multiplex PCR assays, and a multilocus sequence typing (MLST) scheme. A total of 21 A. pittii isolates harbored the metallo-${\beta}$-lactamase (MBL) gene $bla_{IMP-1}$ or $bla_{NDM-1}$. Nineteen isolates containing $bla_{IMP-1}$ were resistant to imipenem and meropenem, but two isolates harboring $bla_{NDM-1}$ were susceptible to them. The sequence types (STs) of the two New Delhi MBL (NDM-1)-producing A. pittii isolates were ST70 and ST207, which differed from the STs (ST63, ST119, ST396, and a novel ST) of the IMP-1-producing A. pittii. This is the first report on NDM-1-producing A. pittii isolates in Korea. Our results emphasize that the study of NDM-1-producing gram-negative bacteria should involve carbapenem-susceptible as well as carbapenem-resistant isolates.

Keywords

References

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