Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Evaluation of horticultural traits and genetic relationship in melon germplasm

멜론 유전자원의 원예형질 특성 및 유연관계 분석

  • Jung, Jaemin (Department of Horticultural Bioscience, Pusan National University) ;
  • Choi, Sunghwan (Department of Horticultural Bioscience, Pusan National University) ;
  • Oh, Juyeol (Gyeongnam Agricultural Research & Extension Services) ;
  • Kim, Nahui (Department of Horticultural Bioscience, Pusan National University) ;
  • Kim, Daeun (Department of Horticultural Bioscience, Pusan National University) ;
  • Son, Beunggu (Department of Horticultural Bioscience, Pusan National University) ;
  • Park, Younghoon (Department of Horticultural Bioscience, Pusan National University)
  • 정재민 (부산대학교 원예생명과학과) ;
  • 최성환 (부산대학교 원예생명과학과) ;
  • 오주열 (경상남도 농업기술원) ;
  • 김나희 (부산대학교 원예생명과학과) ;
  • 김다은 (부산대학교 원예생명과학과) ;
  • 손병구 (부산대학교 원예생명과학과) ;
  • 박영훈 (부산대학교 원예생명과학과)
  • Received : 2015.12.19
  • Accepted : 2015.12.21
  • Published : 2015.12.31
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Abstract

Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).

멜론(Cucumis melo L.) 유전자원 83 품종에 대한 형질특성 및 유전적 다양성을 분석하였다. 형질은 유묘, 잎, 줄기, 화기, 과실, 종자에 대해 총 35개 세부특성을 조사하고, 다변량(MANOVA) 분석을 하였다. 주성분 분석(PCA, principal component analysis) 결과 과중, 과장, 과경, 자엽길이, 종자직경, 종자길이 등 8개의 주성분이 전체 변량의 76.3% 를 나타내었다. 평균연관법(Average linkage method)을 사용한 83개의 멜론의 군집분석(Cluster analysis) 결과 coefficient 0.7에서 5개의 cluster로 분류되었다. Cluster I은 과특성에 있어 가장 높은 측정치를, Cluster II는 당도, Cluster V는 과의 성숙기간이 긴 품종들로 주로 구성되었다. 유전자형 분석은 Cucurbit Genomics Initiative (ICuGI) database에 공시된 15개의 Expressed-sequence Tag-Simple Sequence Repeat (EST-SSR) 마커를 이용하였으며 비가중평균결합법(UPGMA)을 통해 품종간 유연관계를 분석하고 6개의 군으로 분류하였다. 형태적 군집분석 결과와 유전적 군집분석 결과의 상관관계를 조사한 결과 상관계수(r) 값이 -0.11으로 매우 낮게 나타났다.

Keywords

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