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Effect of tissue proliferation and somatic embryo induction in Larix kaempferi following treatment with organic nitrogen sources and plant growth regulators

일본잎갈나무(Larix kaempferi) 유기질소원 및 식물생장조절물질 처리에 따른 조직증식 및 체세포배 유도 효과

  • Kim, Yong Wook (Korea Forest Research Institute (KFRI), Division of Forest Biotechnology) ;
  • Kim, Ji Ah (Korea Forest Research Institute (KFRI), Division of Forest Biotechnology) ;
  • Moon, Heung Kyu (Korea Forest Research Institute (KFRI), Division of Forest Biotechnology) ;
  • Jeong, Su Jin (Korea Forest Research Institute (KFRI), Division of Forest Biotechnology)
  • 김용욱 (국립산림과학원 산림생명공학과) ;
  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 문흥규 (국립산림과학원 산림생명공학과) ;
  • 정수진 (국립산림과학원 산림생명공학과)
  • Received : 2015.09.22
  • Accepted : 2015.12.02
  • Published : 2015.12.31

Abstract

This study was conducted to evaluate the effects of different types and concentrations of organic nitrogen sources (${\small{L}}$-Glutamine and casein hydrolysate, CH) and plant growth regulators (auxins and cytokinins) on embryogenic tissue proliferation and somatic embryo production in L. kaempferi. Overall, the highest tissue fresh weight was obtained at either 2 or 4 weeks in culture when 1,000 mg/L ${\small{L}}$-Glutamine was added to the culture medium, which showed similar results with other treatments. In experiments with different types and concentrations of plant growth regulators on somatic embryo production, the highest production (426.3/90 mg tissue) was found when 0.2 mg/L IBA was added; however, no somatic embryos were induced following treatment with 0.2 mg/L BA or Kinetin. The effect of various concentrations of IBA on somatic embryo production was also tested. The best result (303/90 mg tissue) was obtained when plants were treated with 0.2 mg/L IBA; 1.0 mg/L IBA was also effective (281/90 mg tissue). The lowest result (109.3/90 mg tissue) was obtained with 5.0 mg/L IBA.

본 연구는 낙엽송의 배발생조직 증식 및 체세포배 유도에 영향하는 유기질소원(${\small{L}}$-Glutamine 및 casein hydrolysate, CH), 식물생장조절물질(옥신 및 사이토키니류)의 종류 및 농도의 효과를 평가하기 위해 수행되었다. 배발생조직 증식 비교에서 1,000 mg/L ${\small{L}}$-Glutamine 첨가 시 2주 혹은 4주 모두 가장 높은 조직생중량을 보였고 그 외 처리구에서는 대체로 유사하였다. 체세포배 유도에서는 0.2 mg/L IBA 첨가 시 가장 높은 426.3개를 유도하여 가장 효과적이었으나 0.2 mg/L BA 혹은 Kinetin처리구에서는 전혀 체세포배가 유도되지 않았다. IBA 농도 별 비교에서는 0.2 mg/L(303개)농도가 가장 좋았으며, 1.0 mg농도에서도 281개를 유도하여 체세포배 유도에 효과적이었으나 5.0 mg/L 첨가 시 109.3개로 가장 저조하였다.

Keywords

References

  1. Arnold S von (1987) Improved efficiency of somatic embryogenesis in mature embryos of Picea abies (L) Karst. J Plant Physiol 128:233-244 https://doi.org/10.1016/S0176-1617(87)80237-7
  2. Barrett JD, Park YS, Bonga JM (1997) The effectiveness of various nitrogen sources in white spruce [Picea glauca (Moench)] somatic embryogenesis. Plant Cell Rep 16:411-415
  3. Becwar M, Noland TL, Wyckoff JL (1989) Maturation, germination, and conversion of Norway spruce (Picea abies L.) somatic embryos to plants. In Vitro Cell Develop Biol-Plant 25:575-580 https://doi.org/10.1007/BF02623571
  4. Bozhkov PV, Lebedenko LA, Shiryaeva GA (1992) A pronounced synergistic effect of abscisic acid and 6-benzyladenine on Norway spruce (Picea abies L. Karst) somatic embryo maturation. Plant Cell Rep 11:386-389
  5. Bozhkov PV, Mikhlina SB, Shiryaeva GA, Lebedenko LA (1993) Influence of nitrogen balance of culture medium on Norway spruce (Picea abies (L.) Karst) somatic polyembryogenesis: High frequency establishment of embryonal-suspensor mass lines from mature zygotic embryos. J Plant Physiol 142:735-741 https://doi.org/10.1016/S0176-1617(11)80911-9
  6. Gomez MP, Segura J (1996) Morphogenesis in leaf and single-cell cultures of mature Juniperus oxycedrus. Tree Physiol 16:681-686 https://doi.org/10.1093/treephys/16.8.681
  7. harry IS, Thorpe TA (1991) Somatic embryogenesis and plant regeneration from mature zygotic embryos of red spruce. Bot Gaz 152:446-452 https://doi.org/10.1086/337905
  8. Jain SM, Newton RJ, Soltes EJ (1988) Enhancement of somatic embryogenesis in Norway spruce (Picea abies L.). Theor Appl Genet 76:501-506
  9. Liu C, Xia X, Yin W, Huang L, Zhou J (2006) Shoot regeneration and somatic embryogenesis from needles of redwood (Sequoia sempervirens (D.Don.) Endl.). Plant Cell Rep 25:621-628 https://doi.org/10.1007/s00299-006-0120-y
  10. Ogita S, Sasamoto H, Yeung EC, Thorpe TA (2001) The effect of glutamine on the maintenance of embryogenic cultures of Cryptomeria japonica. In Vitro Cell Develop Biol-Plant 37:268-273 https://doi.org/10.1007/s11627-001-0048-4
  11. Zhang CX, Li Q, Kong L (2007) Induction, development and maturation of somatic embryos in Bunge's pine (Pinus bungeana Zucc. ex Endl.). Plant Cell Tiss Org Cult 91:273-280 https://doi.org/10.1007/s11240-007-9294-4