The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Effects of Hataedock on 2,4-dinitrofluorobenzene Induced Atopic Dermatitis Like Skin Lesion in NC/Nga Mice

하태독법 중 황련감초법이 DNFB로 유발된 NC/Nga 생쥐의 아토피 피부염에 미치는 영향

  • Cha, Ho Yeol (Department of Pediatrics of Korean Medicine, Pusan National University Hospital) ;
  • Ahn, Sang Hyun (Research Institute for Korean Medicine, Pusan National University) ;
  • Jeong, A Ram (Department of Pediatrics of Korean Medicine, Pusan National University Hospital) ;
  • Cheon, Jin Hong (Department of Pediatrics of Korean Medicine, Pusan National University Hospital) ;
  • Park, Sun Young (Department of physiology, college of Korean Medicine, Semyung University) ;
  • Kim, Ki Bong (Department of Pediatrics of Korean Medicine, Pusan National University Hospital)
  • 차호열 (부산대학교한방병원 한방소아과) ;
  • 안상현 (부산대학교 한의과학연구소) ;
  • 정아람 (부산대학교한방병원 한방소아과) ;
  • 천진홍 (부산대학교한방병원 한방소아과) ;
  • 박선영 (세명대학교 한의과대학 생리학교실) ;
  • 김기봉 (부산대학교한방병원 한방소아과)
  • Received : 2015.10.22
  • Accepted : 2015.11.11
  • Published : 2015.11.30
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Abstract

Objectives Hataedock is the treatment that dispels toxic heat and meconium gathered at the fetus for the new born baby by orally administered herbal extracts. The purpose of this study was to evaluate whether Hataedock alleviate inflammatory skin damages in AD-induced NC/Nga mice through regulating of skin barrier maintain and Th2 differentiation. Methods We established an AD model in the 3-week-old NC/Nga mice through the repeated application of DNFB (dinitrochlorobenzene) on days 28, 35, 42 after Hataedock treatment which was orally administered. We identified the changes of skin barrier and Th2 differentiation through the histological and immunohistochemical changes of protein kinase C (PKC), interleukin (IL)-4, degranulated mast cell, Substance P and MMP-9. Results Our results suggested that Hataedock treatment significantly down-regulated levels of PKC by 82% (p < 0.001), as well as IL-4 by 56% (p < 0.001). Hataedock also suppressed mast cell infiltration, ear edema formation. and Substance P in the tissue of NC/Nga mice were decreased by 57% (p < 0.001), and MMP-9 by 55% (p < 0.001). Conclusions These results suggest that Hataedock alleviates AD through the down-regulation of PKC and Th2 cytokines, which are involved in the initial steps of AD development. Hataedock have potential application for the treatment of AD.

Keywords

References

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