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Protective Effect of Liriodendrin Isolated from Kalopanax pictus against Gastric Injury

  • Received : 2014.09.17
  • Accepted : 2014.10.30
  • Published : 2015.01.01

Abstract

In this study, we investigated the inhibitory activities on gastritis and gastric ulcer using liriodendrin which is a constituent isolated from Kalopanax pictus. To elucidate its abilities to prevent gastric injury, we measured the quantity of prostaglandin $E_2$ ($PGE_2$) as the protective factor, and we assessed inhibition of activities related to excessive gastric acid be notorious for aggressive factor and inhibition of Helicobacter pylori (H. pylori) colonization known as a cause of chronic gastritis, gastric ulcer, and gastric cancer. Liriodendrin exhibited higher $PGE_2$ level than rebamipide used as a positive control group at the dose of $500{\mu}M$. It was also exhibited acid-neutralizing capacity (10.3%) and $H^+/K^+$-ATPase inhibition of 42.6% ($500{\mu}M$). In pylorus-ligated rats, liriodendrin showed lower volume of gastric juice ($4.38{\pm}2.14ml$), slightly higher pH ($1.53{\pm}0.41$), and smaller total acid output ($0.47{\pm}0.3mEq/4hrs$) than the control group. Furthermore liriodendrin inhibited colonization of H. pylori effectively. In vivo test, liriodendrin significantly inhibited both of HCl/EtOH-induced gastritis (46.9 %) and indomethacin-induced gastric ulcer (46.1%). From these results, we suggest that liriodendrin could be utilized for the treatment and/or protection of gastritis and gastric ulcer.

Keywords

References

  1. Bae, E. A., Han, M. J., Baek, N. I. and Kim, D. H. (2001) In vitro anti Helicobacter pylori activity of panaxtriol isolated from ginseng. Arch. Pharm. Res. 24, 297-299. https://doi.org/10.1007/BF02975095
  2. Bebb, J., James, M. W. and Atherton, J. (2003) Gastritis and Peptic Ulcer. Medicine 31, 15-18. https://doi.org/10.1383/medc.31.1.15.28592
  3. Choi, C. H., Cha, Y. J., An, C. S., Kim, K. J., Kim, K. C., Moon, S. P., Lee, Z. H. and Min, Y. D. (2004) Molecular mechanisms of heptaplatin effective against cisplatin-resistant cancer cell lines: less involvement of metallothionein. Cancer Cell Int. 4, 6. https://doi.org/10.1186/1475-2867-4-6
  4. Feng, C., Li, B. G., Gao, X. P., Qi, H. Y. and Zhang, G. L. (2010) A new triterpene and an antiarrhythmic liriodendrin from Pittosporum brevicalyx. Arch. Pharm. Res. 33, 1927-1932. https://doi.org/10.1007/s12272-010-1206-1
  5. Hollander, D., Tarnawski, A. and Cummings, D. (1984) Cytoprotectiveaction of antacids against alcohol induced gastric mucosal injury:Morphological ultrasturctural and functional time sequence analysis. Gastroenterology 86, 1114.
  6. Itoh, T., Wakatsuki, Y., Yoshida, M., Usui, T., Matsunaga, Y., Kaneko, S., Chiba, T. and Kita, T. (1999) The vast majority of gastric T cells are polarized to produce T helper 1 type cytokines upon antigenic stimulation despite the absence of Helicobacter pylori infection. Gastroenterology 34, 560-570. https://doi.org/10.1007/s005350050373
  7. Jung, H. J., Park, H. J., Kim, R. G., Shin, K. M., Ha, J., Choi, J. W., Kim, H. J., Lee, Y. S. and Lee, K. T. (2003) In vivo anti-inflammatory and antinociceptive effects of liriodendrin isolated from the stem bark of Acanthopanax senticosus. Planta Med. 69, 610-616. https://doi.org/10.1055/s-2003-41127
  8. Kasuya, Y., Urushidani, T. and Okabe, S. (1979) Effects of various drugs and vagotomy on indomethacin-induced gastric ulcers in the rat. Jpn. J. Pharmacol. 29, 670-673. https://doi.org/10.1254/jjp.29.670
  9. Kato, S., Morie, T. and Yoshida, N. (1995) Synthesis and biological activities of metabolites of mosapride, a new gastroprokinetic agent. Chem. Pharm. Bull. 43, 699-702. https://doi.org/10.1248/cpb.43.699
  10. Kim, J. M., Shin, J. E, Han, M. J., Baek, N. I. and Kim, D. H. (2003) Inhibitory effect of ginseng polyacetylenes on infection and vacuolation of Helicobacter pylori. Nat. prod. sci. 9, 158-160.
  11. Kunikata, T., Araki, H., Takeeda, M., Kato, S. and Takeuchi, K. (2001) Prostaglandin E prevents indomethacin-induced gastric and intestinal damage through different EP receptor subtypes. J. Physiol. Paris 95, 157-163. https://doi.org/10.1016/S0928-4257(01)00021-3
  12. Lee, E., Choi, M. Y., Park, H. J., Cha, B. C. and Cho, S. H. (1995) Chemical constituents and biological activity of Kalopanacis Cortex. Kor. J. Pharmacogn. 26, 122-129.
  13. Malagelada, J. R. and Stanghellini, V. (1985) Manometric evaluation of functional upper gut symptoms. Gastoenterology 88, 1223-1231. https://doi.org/10.1016/S0016-5085(85)80083-4
  14. Mcquaid, K. R. and Isenberg, J. I. (1992) Medical therapy of peptic ulcer disease. Surg. Clin. North Am. 72, 285-316. https://doi.org/10.1016/S0039-6109(16)45680-X
  15. Mizui, T. and Dodeuchi, M. (1983) Effect of polyamines on acidified EtOH-induced gastric lesion in rats. Jpn. J. Pharmacol. 33, 939-945. https://doi.org/10.1254/jjp.33.939
  16. Muhammad, J. S., Sugiyama, T. and Zaidi, S. F. (2013) Gastric pathophysiological ins and outs of Helicobacter pylori. J. Pak. Med. Assoc. 63, 1528-1533.
  17. Odaka, T., Suzuki, T., Seza, A., Yamaguchi, T. and Saisho, H. (2006) Serotonin 5- HT4 receptor agonist (mosapride citrate). Nihon Rinsho. 64, 1491-1494.
  18. Raghunath, A. S., O'Morain, C. and Mcloughlin, R. C. (2005) The longterm use of proton-pump inhibitors. Aliment. Pharmacol. Ther. 22, 55-63. https://doi.org/10.1111/j.1365-2036.2005.02611.x
  19. Rovert, A. (1979) Cytoprotection by prostaglandins. Gastroenterology 77, 761-767.
  20. Rovert, A., Nezaims, J. E., Lancaste, C. and Hanchar, A. J. (1979) Cytoprotection by prostaglandins in rats; Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl and thermal injury. Gastroenterology 77, 433-443.
  21. Saccomani, G., Barcellona, M. L. and Sachs, G. (1981) Reactivity of gastric ($H^{+}/K^{+}$)-ATPase to N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. J. Biol. Chem. 256, 12405-12410.
  22. Scarpignato, C., Capovilla, T. and Bertaccini, G. (1980) Action of caerulein on gastric emptying of the conscious rat. Arch. Int. Pharmacodyn. Ther. 246, 286-294.
  23. Seol, S. Y. (2006) Mechanism of actions and clinical applications of proton pump inhibitors. Korean J. Gastroenterol. 48, 4-8.
  24. Shay, H., Komarov, S. A., Fels, S. S. and Meranze, D. (1945) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterol. 4, 43-61.
  25. Tarnawski, A., Hollander, D., Gergely, H. and Stachura, J. (1985) Comparison of antacid, sucralfate, cimetidine and ranitidine in protection of the gastric mucosa against EtOH injury. Am. J. Med. 79, 19-23.
  26. van Doorn, N. E., van Rees, E. P., Namavar, F. and de Graaff, J. (1998) Local cellular immune response in the acute phase of gastritis in mice induced chemically and by Helicobacter pylori. J. Med. Microbiol. 47, 863-870. https://doi.org/10.1099/00222615-47-10-863
  27. Walker, J., Hell, J., Liszt, K. I., Dresel, M., Pignitter, M., Hofmann, T. and Somoza, V. (2012) Identification of beer bitter acids regulating mechanisms of gastric acid secretion. J. Agric. Food Chem. 60, 1405-1412. https://doi.org/10.1021/jf204306z
  28. Wallmark, B., Larsson, H. and Humble, L. (1985) The relationship between gastric acid secretion and gastric $H^{+},K^{+}$-ATPase activity. J. Biol. Chem. 260, 13681-13684.

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