The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Analysis of Mycological Characteristics and Lignocellulose Degradation of Gyrodontium sacchari

헌구두솔버섯균의 균학적 특성 및 목질계 섬유소의 분해 특성 분석

  • Park, In-Cheol (Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) ;
  • Seok, Soon-Ja (Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Jeong-Seon (Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) ;
  • Yoo, Jae-Hong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA) ;
  • Ahn, Jae-Hyung (Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA)
  • 박인철 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 석순자 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김정선 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 유재홍 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 안재형 (농촌진흥청 국립농업과학원 농업미생물과)
  • Received : 2015.11.18
  • Accepted : 2015.11.24
  • Published : 2015.12.31
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Abstract

Two fungal strains were isolated from rods of Quercus sp. (NAAS02335) and Pinus densiflora (NAAS05299) in Korea. These strains were identified as Gyrodontium sacchari by their morphological and mycological characteristics. The optimal growth temperature of NAAS02335 and NAAS05299 are $25^{\circ}C$ and $30^{\circ}C$, respectively. Production of cellulase, xylanase, and ligninase was tested on agar media supplemented dyes or substrates. Production of cellulase and xylanase of NAAS05299 was higher than those of NAAS02335, however ligninase activity of NAAS02335 was higher than that of NAAS05299. The activities of cellulase, xylanase, and amylase of strain NAAS05299 were estimated at 6.7~10.2 times higher than that of NAAS02335. Laccase activity was only estimated by strain NAAS02335. The lignocellulytic enzymes are induced by substrates such as rice straw, wooden chips of pine, oak, and poplar. The NAAS05299 was able to degrade filter paper completely after 4 weeks of culturing in liquid media containing a piece of filter paper at $28^{\circ}C$ with continuous shaking. NAAS05299 was able to degrade rice straw, pine chips, and oak chips after 4 months in solid culture, however NAAS02335 decomposed only rice straw among tested 4 kinds of biomass.

참나무와 소나무 목재에서 분리한 Gyrodontium sacchari 균주에 대한 균학적 특성과 목질계 섬유소 분해력을 검정하였다. 균주는 참나무와 소나무 목재에서 분리하였으며 배지는 potato dextrose agar (PDA)에서 가장 생장이 좋았고 생장온도는 참나무에서 분리한 NAAS02335 균주는 $25^{\circ}C$에서, 소나무 목재에서 분리한 NAAS05299 균주는 $30^{\circ}C$에서 가장 우수한 생장을 보였다. 섬유소 분해 효소인 cellulase와 xylanase, amylase의 활성은 G. sacchari NAAS05299 균주가 6.7~12.8배 더 높았으며 리그닌 분해 효소는 G. sacchari NAAS02335 균주가 3.7~138.5배 활성이 더 높았으며 목질계 섬유소를 탄소원으로 첨가하였을 때 효소의 활성은 월등히 증가하였다. 목질계 바이오매스 분해력을 검정한 결과 G. sacchari NAAS05299 균주는 filter paper를 4주만에 완전히 분해하였고 볏짚과 미송, 참나무를 분해하였으나 G. sacchari NAAS02335 균주는 볏짚에서만 분해력을 나타내어 G. sacchari NAAS05299 균주가 더 우수한 바이오매스 분해 효과를 나타내었다.

Keywords

References

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