Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Assessment of Palm Press Fibre and Sawdust-Based Substrate Formulas for Efficient Carpophore Production of Lentinus squarrosulus (Mont.) Singer

  • Osibe, Dandy Ahamefula (Mushroom Research Unit, Department of Plant Science and Biotechnology, University of Nigeria) ;
  • Chiejina, Nneka Virginia (Mushroom Research Unit, Department of Plant Science and Biotechnology, University of Nigeria)
  • Received : 2015.09.11
  • Accepted : 2015.11.29
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Development of efficient substrate formulas to improve yield and shorten production time is one of the prerequisites for commercial cultivation of edible mushrooms. In this study, fifteen substrate formulas consisting of varying ratios of palm press fibre (PPF), mahogany sawdust (MS), Gmelina sawdust, wheat bran (WB), and fixed proportions of 1% calcium carbonate ($CaCO_3$) and 1% sucrose were assessed for efficient Lentinus squarrosulus production. Proximate compositions of mushrooms produced on the different substrate formulas were also analysed and compared. Substrate formulations containing 85% PPF, 13% WB, 1% $CaCO_3$, and 1% sucrose were found to produce the highest carpophore yield, biological efficiency and size (206.5 g/kg, 61.96%, and 7.26 g, respectively). Days to production (first harvest) tended to increase with an increase in the amount of WB in the substrate formulas, except for PPF based formulas. The addition of WB in amounts equivalent to 8~18% in substrate formulas containing 80~90% PPF resulted in a decrease in the time to first harvest by an average of 17.7 days compared to 80~90% MS with similar treatment. Nutritional content of mushrooms was affected by the different substrate formulas. Protein content was high for mushrooms produced on formulas containing PPF as the basal substrate. Thus, formulas comprising PPF, WB, $CaCO_3$, and sucrose at 85% : 13% : 1% : 1%) respectively could be explored as starter basal ingredients for efficient large scale production of L. squarrosulus.

Keywords

References

  1. Isikhuemhen OS, Nerud F, Vilgalys R. Cultivation studies on wild and hybrid strains of Pleurotus tuberregium (Fr.) Sing. on wheat straw substrate. World J Microbiol Biotechnol 2000; 16:431-5. https://doi.org/10.1023/A:1008958319418
  2. Isikhuemhen OS, Mikiashvilli NA. Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids. J Ind Microbiol Biotechnol 2009;36:1353-62. https://doi.org/10.1007/s10295-009-0620-1
  3. Royse DJ, Rhodes TW, Ohga S, Sanchez JE. Yield, mushroom size and time to production of Pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. Bioresour Technol 2004;91:85-91. https://doi.org/10.1016/S0960-8524(03)00151-2
  4. Isikhuemhen OS, Mikiashvili NA, Adenipekun CO, Ohimain EI, Shahbazi G. The tropical white rot fungus, Lentinus squarrosulus Mont.: lignocellulolytic enzymes activities and sugar release from cornstalks under solid state fermentation. World J Microbiol Biotechnol 2012;28:1961-6. https://doi.org/10.1007/s11274-011-0998-6
  5. Kadiri M. Toxicological evaluation of Lentinus squarrosulus Mont. (Polyporales), an indigenous Nigerian mushroom. Int J Med Mushrooms 2005;7:416-7.
  6. Zhou S, Tang QJ, Zhang Z, Li CH, Cao H, Yang Y, Zhang JS. Nutritional composition of three domesticated culinarymedicinal mushrooms: Oudemansiella sudmusida, Lentinus squarrosulus, and Tremella aurantialba. Int J Med Mushrooms 2015;17:43-9. https://doi.org/10.1615/IntJMedMushrooms.v17.i1.50
  7. Okhuoya JA, Akpaja EO, Oghenekaro A. Cultivation of Lentinus squarrosulus (Mont.) Singer on sawdust of selected tropical tree species. Int J Med Mushrooms 2005;7:440-1.
  8. Adesina FC, Fasidi IO, Adenipekun OC. Cultivation and fruit body production of Lentinus squarrosulus Mont. (Singer) on bark and leaves of fruit trees supplemented with agricultural waste. Afr J Biotechnol 2011;10:4608-11.
  9. Royse DJ. Influence of spawn rate and commercial delayed release nutrient levels on Pleurotus cornucopiae (oyster mushroom) yield, size, and time to production. Appl Microbiol Biotechnol 2002;58:527-31. https://doi.org/10.1007/s00253-001-0915-2
  10. Riansa-Ngawong W, Prasertsan P. Optimization of furfural production from hemicellulose extracted from delignified palm pressed fiber using a two-stage process. Carbohydr Res 2011;346:103-10. https://doi.org/10.1016/j.carres.2010.10.009
  11. Abd Razak DL, Abdullah N, Khir Johari NM, Sabaratnam V. Comparative study of mycelia growth and sporophore yield of Auricularia polytricha (Mont.) Sacc on selected palm oil wastes as fruiting substrate. Appl Microbiol Biotechnol 2013; 97:3207-13. https://doi.org/10.1007/s00253-012-4135-8
  12. Ilukor JO, Oluka SO. Carbon-to-nitrogen ratios in agricultural residues. Environ Monit Assess 1995;38:271-5.
  13. Goering HK, Van Soest PJ. Forage fibre analyses: apparatus, reagents, procedures, and some applications. Agricultural handbook 379. Washington, DC: U.S. Government Printing Office; 1970.
  14. Association of Official Analytical Chemists. Official method of analysis of the Association of Official Analytical Chemists. 14th ed. Washington, DC: Association of Official Analytical Chemists; 1990.
  15. Shashirekha MN, Rajarathnam S, Bano Z. Effects of supplementing rice straw growth substrate with cotton seeds on the analytical characteristics of the mushroom, Pleurotus florida (Block & Tsao). Food Chem 2005;92:255-9. https://doi.org/10.1016/j.foodchem.2004.07.034
  16. Michael HW, Bultosa G, Pant LM. Nutritional contents of three edible oyster mushrooms grown on two substrates at Haramaya, Ethiopia, and sensory properties of boiled mushroom and mushroom sauce. Int J Food Sci Technol 2011;46:732-8. https://doi.org/10.1111/j.1365-2621.2010.02543.x
  17. Royse DJ, Sanchez-Vazquez JE. Influence of substrate woodchip particle size on shiitake (Lentinula edodes) yield. Bioresour Technol 2001;76:229-33. https://doi.org/10.1016/S0960-8524(00)00110-3
  18. Ohga S. Growth rate of mycelium of shiitake, Lentinus edodes, in relation to water potential of medium. J Fac Agric Kyushu Univ 1990;34:413-20.
  19. Yang FC, Hsieh C, Chen HM. Use of stillage grain from a rice-spirit distillery in the solid state fermentation of Ganoderma lucidum. Process Biochem 2003;39:21-6. https://doi.org/10.1016/S0032-9592(02)00255-8
  20. Moonmoon M, Uddin MN, Ahmed S, Shelly NJ, Khan MA. Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on saw dust and rice straw in Bangladesh. Saudi J Biol Sci 2010;17:341-5. https://doi.org/10.1016/j.sjbs.2010.05.004
  21. Yang W, Guo F, Wan Z. Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull. Saudi J Biol Sci 2013;20:333-8. https://doi.org/10.1016/j.sjbs.2013.02.006
  22. Ozcelik E, Peksen A. Hazelnut husk as a substrate for the cultivation of shiitake mushroom (Lentinula edodes). Bioresour Technol 2007;98:2652-8. https://doi.org/10.1016/j.biortech.2006.09.020
  23. Shen Q, Royse DJ. Effects of nutrient supplements on biological efficiency, quality and crop cycle time of maitake (Grifola frondosa). Appl Microbiol Biotechnol 2001;57:74-8. https://doi.org/10.1007/s002530100748
  24. Tshinyangu KK. Effect of grass hay substrate on nutritional value of Pleurotus ostreatus var. columbinus. Nahrung 1996; 40:79-83. https://doi.org/10.1002/food.19960400207
  25. Wang D, Sakoda A, Suzuki M. Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresour Technol 2001;78:293-300. https://doi.org/10.1016/S0960-8524(01)00002-5
  26. Crisan EV, Sands A. Nutritional value. In: Chang ST, Hayes WA, editors. The biology and cultivation of edible mushrooms. New York: Academic Press; 1978. p. 137-65.
  27. Yang JH, Lin HC, Mau JL. Non-volatile taste components of several commercial mushrooms. Food Chem 2001;72:465-71. https://doi.org/10.1016/S0308-8146(00)00262-4
  28. Okoro IO, Achuba FI. Proximate and mineral analysis of some wild edible mushrooms. Afr J Biotechnol 2012;11:7720-4.
  29. Obodai M, Ferreira IC, Fernandes A, Barros L, Mensah DL, Dzomeku M, Urben AF, Prempeh J, Takli RK. Evaluation of the chemical and antioxidant properties of wild and cultivated mushrooms of Ghana. Molecules 2014;19:19532-48. https://doi.org/10.3390/molecules191219532