Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지
DOI QR코드

DOI QR Code

Recovery of In Vivo Matured Oocytes from a Bitch with Hydrometra

자궁수종이 있는 개에서 체내 성숙 난자의 회수

  • 김민정 (서울대학교 수의학과 수의산과 및 생명공학) ;
  • 조영광 (서울대학교 수의학과 수의산과 및 생명공학) ;
  • 강상철 ((주)옵티팜) ;
  • 오현주 (서울대학교 수의학과 수의산과 및 생명공학) ;
  • 김건아 (서울대학교 수의학과 수의산과 및 생명공학) ;
  • ;
  • 최유빈 (서울대학교 수의학과 수의산과 및 생명공학) ;
  • 이석희 (서울대학교 수의학과 수의산과 및 생명공학) ;
  • 김현일 ((주)옵티팜) ;
  • 이병천 (서울대학교 수의학과 수의산과 및 생명공학)
  • Accepted : 2015.12.14
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

One year old mixed-breed bitch was examined to retrieve in vivo matured oocytes. Laparotomy was performed 72 hr after ovulation determined by serum progesterone concentration, and abnormally enlarged left uterus horn was found. Both ovaries had eight corpus lutea, and a total 16 in vivo matured oocytes having perivitelline space within $25{\mu}m$, polar body, and metaphase II nucleus were recovered by flushing oviducts. This is the first study to confirm in vivo maturation of oocytes from a bitch with hydrometra, which suggests that oocytes recovered from canids with reproductive disease could be valuable sources for assisted reproductive technologies.

한살령의 잡종 암캐에서 체내 성숙된 난자를 회수하기 위한 실험이 진행되었다. 혈청 프로게스테론 농도를 이용하여 배란 후 약 72시간 뒤에 개복술을 시행하였고, 비정상적으로 확장된 왼쪽 자궁각이 발견되었다. 양쪽 난소는 각각 여덟 개의 황체를 가지고 있었고, 배지를 난관에 관류시켜서 총 16개의 난자를 회수하였다. 모든 난자는 극체가 돌출되어 있었고, 25 um 미만의 난황주위 공간과 제 2중기의 핵을 가지고 있었다. 본 증례는 자궁수종을 가진 암캐에서 체내 성숙된 난자를 확인한 최초의 연구이며, 생식기 관련 질병을 가진 개과 동물에서 회수된 난자가 보조 생식 기술에 사용될 수 있음을 의미한다.

Keywords

References

  1. Chastant-Maillard S, Chebrout M, Thoumire S, Saint-Dizier M, Chodkiewicz M, Reynaud K. Embryo biotechnology in the dog: a review. Reprod Fertil Dev 2010; 22: 1049-1056. https://doi.org/10.1071/RD09270
  2. Concannon PW. Endocrinologic control of normal canine ovarian function. Reprod Domest Anim 2009; 44 Suppl 2: 3-15. https://doi.org/10.1111/j.1439-0531.2009.01414.x
  3. Fransson B, Lagerstedt AS, Hellmen E, Jonsson P. Bacteriological findings, blood chemistry profile and plasma endotoxin levels in bitches with pyometra or other uterine diseases. J Vet Med A 1997; 44: 417-426. https://doi.org/10.1111/j.1439-0442.1997.tb01127.x
  4. Jang G, Kim MK, Oh HJ, Hossein MS, Fibrianto YH, Hong SG, Park JE, Kim JJ, Kim HJ, Kang SK, Kim DY, Lee BC. Birth of viable female dogs produced by somatic cell nuclear transfer. Theriogenology 2007; 67: 941-947. https://doi.org/10.1016/j.theriogenology.2006.11.006
  5. Johnston SD, Root Kustritz MV, Olson PS, Disorders of the canine uterus and uterine tubes (oviducts). In: Canine and feline theriogenology, 1st ed. Philadelphia: WB Saunders Company. 2001: 206-207.
  6. Kim MJ, Oh HJ, Kim GA, Jo YK, Choi J, Lee BC. Application of chemiluminescence enzyme immunoassay method to collect in vivo matured oocyte in dog cloning. J Vet Clin 2014; 31: 267-271. https://doi.org/10.17555/ksvc.2014.08.31.4.267
  7. Kim MJ, Oh HJ, Park JE, Hong SG, Kang JT, Koo OJ, Kang SK, Jang G, Lee BC. Influence of oocyte donor and embryo recipient conditions on cloning efficiency in dogs. Theriogenology 2010; 74: 473-478. https://doi.org/10.1016/j.theriogenology.2010.03.001
  8. Kim MJ, Oh HJ, Park JE, Kim GA, Park EJ, Jang G, Lee BC. Effects of mineral supplements on ovulation and maturation of dog oocytes. Theriogenology 2012; 78: 110-115. https://doi.org/10.1016/j.theriogenology.2012.01.026
  9. Matsuu A, Hashizume T, Kanda T, Nagano M, Sugiyama A, Okamoto Y, Hikasa Y. A case of persistent Müllerian duct syndrome with Sertoli cell tumor and hydrometra in a dog. J Vet Med Sci 2009; 71: 379-381. https://doi.org/10.1292/jvms.71.379
  10. Oh HJ, Hong SG, Park JE, Kang JT, Kim MJ, Kim MK, Kang SK, Kim DY, Jang G, Lee BC. Improved efficiency of canine nucleus transfer using roscovitine-treated canine fibroblasts. Theriogenology 2009; 72: 461-470. https://doi.org/10.1016/j.theriogenology.2009.03.016
  11. Paoloni M, Khanna C. Reprod Fertil DevTranslation of new cancer treatments from pet dogs to humans. Nat Rev Cancer 2008; 8: 147-156. https://doi.org/10.1038/nrc2273
  12. Payan-Carreira R, Pina J, Costa M, Seixas F, Pires M. Oestrogen receptors in a case of hydrometra in a bitch. Vet Rec 2006; 158: 487. https://doi.org/10.1136/vr.158.14.487
  13. Peat JR, Reik W. Incomplete methylation reprogramming in SCNT embryos. Nature genetics 2012; 44: 965-966. https://doi.org/10.1038/ng.2393
  14. Pretzer S. Clinical presentation of canine pyometra and mucometra: a review. Theriogenology 2008; 70: 359-363. https://doi.org/10.1016/j.theriogenology.2008.04.028
  15. Sontas BH, Toydemir FS, Erdogan O, Sennazli G, Ekici H. Inguinal herniation with hydrometra/mucometra in a poodle bitch. Can Vet J 2013; 54: 840.