대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)

  • 제47권4호
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  • Pages.286-291
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  • 2015
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  • 1738-3544(pISSN)
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  • 2288-1662(eISSN)
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)
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한국 된장에서 Equol의 검출 및 미생물 동정

Bacterial Identification and Detection of Equol in Korean Soybean Paste

  • 우승균 (순천향대학교 일반대학원 의료과학과) ;
  • 이소연 (순천향대학교 일반대학원 의료과학과) ;
  • 최고운 (순천향대학교 의료과학대학 임상병리학과) ;
  • 홍유진 (순천향대학교 의료과학대학 임상병리학과) ;
  • 이소민 (순천향대학교 의료과학대학 임상병리학과) ;
  • 박강균 (서울성모병원 진단검사의학팀) ;
  • 엄용빈 (순천향대학교 일반대학원 의료과학과)
  • Woo, Seung-Gyun (Department of Medical Science, College of Medical Sciences, SoonChunHyang University) ;
  • Lee, So-Yeon (Department of Medical Science, College of Medical Sciences, SoonChunHyang University) ;
  • Choi, Go-Woon (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Hong, You-Jin (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Lee, So-Min (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Park, Kang Gyun (Department of Laboratory Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea) ;
  • Eom, Yong-Bin (Department of Medical Science, College of Medical Sciences, SoonChunHyang University)
  • 투고 : 2015.07.30
  • 심사 : 2015.11.05
  • 발행 : 2015.12.30
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초록

에쿠올은 인간의 건강에 유익한 효과를 나타낸다. 발효된 콩 식품들은 에쿠올을 함유하고 있으며, 많은 미생물들이 에쿠올 생산과정에 참여하는 것으로 밝혀졌다. 본 연구에서는 한국의 전통 발효 식품인 된장에 대해 조사하였다. 먼저 서로 다른 제조자로부터 수집 된 37개의 된장 샘플들을 대상으로 에쿠올의 농도를 측정하기 위해 LC-MS/MS를 시행하였다. 측정 결과 3개의 된장 샘플에서 에쿠올이 검출되었고, 507 ng/100 g의 농도가 가장 높게 나타났다. 에쿠올을 함유한 된장에서 15개의 미생물 종들이 16S rRNA gene sequence analysis와 2개의 MALDI-TOF MS분석법에 의해 분리, 동정되었으며 Bacillus spp, Paenibacillus spp, Tetragenococcus spp, Stapylococcus spp, and Clostridium species들이 가장 우세한 미생물들이었다. 이 연구결과로 한국의 전통 발효식품인 된장에서도 에쿠올이 검출되었음을 확인하였다.

Equol has beneficial effects on human health. Fermented soy products contain equol, and many microbes participate in the equol production process. This study investigated fermented Korean soybean paste, doenjang. Thirty seven doenjang samples collected from different manufacturers were examined. Equol was detected in 3 samples (D2, D13, and D19) at the maximum content of 507 ng/100 g by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fifteen microbial species were isolated and identified by 16S rRNA gene sequence analysis and by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Bacillus spp, Paenibacillus spp, Tetragenococcus spp, Stapylococcus spp, and Clostridium species were the predominant bacteria in equol containing doenjang samples.

키워드

참고문헌

  1. Abiru Y, Kumemura M, Ueno T, Uchiyama S, Masaki K. Discovery of an S-equol rich food stinky tofu, a traditional fermented soy product in Taiwan. Int J Food Sci Nutr. 2012,63:964-970. https://doi.org/10.3109/09637486.2012.687369
  2. Abiru Y, Ueno T, Uchiyama S. Isolation and characterization of novel S-equol-producing bacteria from brines of stinky tofu, a traditional fermented soy food in Taiwan. Int J Food Sci Nutr. 2013,64:936-943. https://doi.org/10.3109/09637486.2013.816936
  3. Adlercreutz H. Phyto-oestrogens and cancer. Lancet Oncol. 2002,3:364-373. https://doi.org/10.1016/S1470-2045(02)00777-5
  4. Antignac JP, Cariou R, Le Bizec B, Andre F. New data regarding phytoestrogens content in bovine milk. Food Chem. 2004,87:275-281. https://doi.org/10.1016/j.foodchem.2003.12.013
  5. Bowey E, Adlercreutz H, Rowland I. Metabolism of isoflavones and lignans by the gut microflora: a study in germ-free and human flora associated rats. Food Chem Toxicol. 2003,41:631-636. https://doi.org/10.1016/S0278-6915(02)00324-1
  6. Jin JS, Kitahara M, Sakamoto M, Hattori M, Benno Y. Slackia equolifaciens sp. nov., a human intestinal bacterium capable of producing equol. Int J Syst Evol Microbiol. 2010,60:1721-1724. https://doi.org/10.1099/ijs.0.016774-0
  7. Jou HJ, Tsai PJ, Tu JH, Wu WH. Stinky tofu as a rich source of bioavailable S-equol in Asian diets. J Funct Foods. 2013,5:651-659. https://doi.org/10.1016/j.jff.2013.01.006
  8. Kim SM, Sung JY, Choi SG. Molecular Characterization of Escherichia coli Isolates from Humans and Chickens in the Chungcheong Area Using MLST Analysis. Korean J Clin Lab Sci. 2015,47:71-77. https://doi.org/10.15324/kjcls.2015.47.2.71
  9. Maruo T, Sakamoto M, Ito C, Toda T, Benno Y. Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella. Int J Syst Evol Microbiol. 2008,58:1221-1227. https://doi.org/10.1099/ijs.0.65404-0
  10. Matthies A, Clavel T, Gutschow M, Engst W, Haller D, Blaut M, et al. Conversion of daidzein and genistein by an anaerobic bacterium newly isolated from the mouse intestine. Appl Environ Microbiol. 2008,74:4847-4852. https://doi.org/10.1128/AEM.00555-08
  11. Sathyamoorthy N, Wang TTY. Differential effects of dietary phyto-oestrogens daidzein and equol on human breast cancer MCF-7 cells. Eur J Cancer. 1997,33:2384-2389. https://doi.org/10.1016/S0959-8049(97)00303-1
  12. Setchell KD, Clerici C, Lephart ED, Cole SJ, Heenan C, Castellani D, et al. S-equol, a potent ligand for estrogen receptor ${\beta}$, is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora. Am J Clin Nutr. 2005,81:1072-1079. https://doi.org/10.1093/ajcn/81.5.1072
  13. Yang HJ, Kwon DY, Chung KR, Park S, Pak V. Fermented soybean products and their bioactive compounds (El-Shemy HA. Ed). 2011, pp 21-59. Intech publishing, Croatia.
  14. Yoo SK, Cho WH, Kang SM, Lee SH. Isolation and identification of microorganisms in Korean traditional soybean paste and soybean sauce. Korean J Appl Microbiol Biotechnol. 1999,27:113-117.