Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Der p 1 Inhibits Spontaneous Neutrophil Apoptosis by Cytokine Secretion of Normal and Allergic Lymphocytes

Der p 1에 의한 정상인과 알레르기 환자의 림프구에서 사이토카인 분비를 통한 자발적인 호중구 세포고사 억제

  • Kim, In Sik (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Lee, Na Rae (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Lee, Ji-Sook (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • 김인식 (대전 을지대학교 임상병리학과) ;
  • 이나래 (대전 을지대학교 임상병리학과) ;
  • 이지숙 (원광보건대학교 임상병리학과)
  • Received : 2014.10.07
  • Accepted : 2014.10.26
  • Published : 2015.12.30
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Abstract

Dermatophagoides pteronissinus (DP) is one of the house dust mites (HDM) associated with allergic diseases. The cysteine protease Der p 1 from DP is a powerful allergen. The pathogenic mechanism of allergy is involved in cytokine secretion of lymphocytes and spontaneous apoptosis of neutrophils. In this study, we investigated whether Der p 1 induces cytokine secretion of lymphocytes and if the release of cytokines is associated with regulation of neutrophil apoptosis. In normal and allergic subjects, Der p 1 increased IL-6, IL-8, MCP-1, and GM-CSF release in a time-dependent course. Supernatants collected from normal and allergic neutrophils after Der p 1 stimulation suppressed the apoptosis of normal and allergic neutrophils, although Der p 1 alone has no effect on neutrophils. Der p 1 suppressed neutrophil apoptosis in coculture of normal neutrophils with normal lymphocytes. Der p 1 more strongly suppressed apoptosis of allergic neutrophils cocultured with allergic lymphocytes than normal neutrophils cocultured with normal lymphocytes. In summary, Der p 1 increases the secretion of cytokines, which has anti-apoptotic effects on neutrophils of normal and allergic subjects. These results will contribute to elucidate the pathogenic mechanism of allergic diseases.

Dermatophagoides pteronissinus (DP)는 알레르기 질환과 연관이 있는 집먼지 진드기 중 하나이다. 집먼지 진드기에 의해 생성되는 Cystein 단백질분해효소(Derp-1)가 강력한 알레르겐으로 작용한다. 알레르기 병인기전은 림프구의 사이토카인 분비와 호중구의 자발적인 세포고사와 연관이 있다. 본 연구에서는 Derp-1이 림프구에서 사이토카인 분비를 유도하는지 여부와 이에 의해 호중구 세포고사 조절에 영향을 주는지를 실험 해보았다. 정상인과 알레르기 환자의 림프구에서, Derp-1에 의해 IL-6, IL-8, MCP-1그리고 GM- CSF의 분비가 증가됨을 보였다. Derp-1이 단독으로 호중구에 영향을 주는 것은 아니지만, Derp-1으로 호중구를 자극한 뒤 모은 상층액이 호중구의 세포고사를 억제시킴을 확인하였다. 정상인의 호중구와 림프구를 co-culture하여 Derp-1을 처리한 결과 호중구의 세포고사가 억제되었고, 이보다 알레르기 환자에서 시행되어진 것이 그 효과가 더 크게 나타났다. 즉, Derp-1은 사이토카인의 분비를 증가시키고, 이로 인해 정상인과 알레르기 환자의 호중구의 세포고사를 억제시킨다. 이를 통해 알레르기 질환의 병인기전을 밝히는데 유용한 자료가 될 수 있을 것으로 사료된다.

Keywords

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