Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Effect of Smilax China L. Extract on Cultured NIH3T3 Fibroblasts Damaged by Mercury as Allergic Contact Dermatitis Inducer

알러지성 접촉피부염 유발제인 수은으로 손상된 배양 NIH3T3 섬유모세포에 대한 청미래덩굴 추출물의 효과

  • Han, Sun-Hee (Department of Nursing, Wonkwang Health Science University) ;
  • Jekal, Seung-Joo (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • Received : 2015.11.01
  • Accepted : 2015.11.26
  • Published : 2015.12.30
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Abstract

In order to examine the effect of Smilax china L. (SC) extract on the cytotoxicity of methymercuric chloride (MMC), allergic contact dermatitis, The cytotoxicity of MMC was assessed after cultured NIH3T3 fibroblasts were treated with various concentrations of MMC for 72 hours. And also, the following results were obtained by measuring the antioxidative effect of SC extract on the cytotoxicity of MMC. In this study, MMC remarkably decreased the cell viability of NIH3T3 fibroblasts in a dose-dependent manner, and MMC was seen to be highly-toxic below 100 uM of $XTT_{50}$ value. In addition, the toxicity of MMC was involved in oxidative stress via a blockage of MMC-induced cytotoxicity by vit. E as antioxidant. In the protective effect of SC extract on MMC-induced cytotoxicity, SC extract defended the cytotoxicity of MMC by a significant increase of cell viability which was decreased by MMC-induced cytotoxicity. It also showed antioxidative effects such as electron donating ability (EDA), superoxide dismutase (SOD)-like activity (SLA) and the lipid peroxidation activity (LPA). From these results, the natural component as SC extract may be a putative resource as the antioxidative agent for the treatment of inflammatory skin disease associated with the oxidative stress.

알러지성 접촉피부염 유발제인 수은의 독성에 대한 청미래덩굴(Smilax china L.) 추출물의 영향을 조사하기 위하여 배양 NIH3T3 섬유모세포에 여러 농도의 메틸수은(methylmercuric chloride, MMC)을 72시간 동안 처리한 후 이들의 세포독성을 조사하였다. 또한, MMC의 독성에 대한 청미래덩굴 추출물의 보호효과를 항산화 측면에서 조사하여 다음과 같은 결론을 얻었다. MMC는 농도 의존적으로 배양 NIH3T3 섬유모세포의 세포생존율을 유의하게 감소하였으며 $XTT_{50}$값이 100 uM 이하로서 고독성(highly-toxic)인 것으로 나타났다. 또한, MMC의 독성이 항산화제인 vit. E에 의하여 방어됨으로서 MMC의 독성에 산화적 손상이 관여하고 있는 것으로 나타났다. 한편, MMC의 세포독성에 대한 청미래덩굴 추출물의 방어효과에 있어서 청미래덩굴 추출물은 MMC에 의하여 감소된 세포생존율을 유의하게 증가시킴으로서 MMC의 독성을 방어하였다. 또한, 청미래덩굴 추출물은 전자공여능(EDA)을 비롯한 SOD-유사 활성(SLA) 및 지질과산화능(LPA)와 같은 항산화 효과를 나타냈다. 이상의 결과로부터 청미래덩굴과 같은 천연 성분은 산화적 손상과 관련된 염증성 피부질환의 치료를 위한 항산화제로서의 미래 가능한 천연소재라고 생각된다.

Keywords

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