Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Water Extract from Rice Bran Fermented with Lactobacillus plantarum Hong Inhibits Thromboxane A2 Production Associated Microsomal Enzyme Activity in Human Platelets

  • Kim, Hyun-Hong (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering Inje University) ;
  • Hong, Jeong Hwa (Department of Smart Foods and Drugs, Inje University) ;
  • Ingkasupart, Pajaree (Department of Smart Foods and Drugs, Inje University) ;
  • Lee, Dong-Ha (Department of Biomedical Laboratory Science, Korea Nazarene University) ;
  • Yeo, DaNa (Department of Health Science & Technology, Inje University) ;
  • Park, Hwa-Jin (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering Inje University)
  • Received : 2015.10.09
  • Accepted : 2015.12.11
  • Published : 2015.12.31
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Abstract

In this study, we investigated the effect of rice bran water extract fermented with Lactobacillus plantarum Hong (RBLw), on activities of cyclooxygenase-1 (COX-1) and thromboxane $A_2$ synthase (TXAS), thromboxane $A_2$ ($TXA_2$) production associated microsomal enzymes and evaluated its the antiplatelet effect. RBLw, containing 13.5 mg of ferulic acid, dose-dependently inhibited ADP-induced platelet aggregation, and inhibited the production of $TXA_2$, an aggregation molecule. In addition, RBLw directly inhibited COX-1 activity in a dose-dependent manner, but not TXAS activity in platelet microsomal fraction having cytochrome c reductase (an endoplasmic reticulum marker enzyme) activity and expressing COX-1 (72 kDa) and TXAS (60.5 kDa) proteins. These results suggest that RBLw selectively inhibited the activity of COX-1 rather than TXAS to attenuate $TXA_2$ production in ADP-activated platelets. Thus, we demonstrate that RBLw might have direct COX-1 antagonistic function for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.

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References

  1. Baek SE, Kim JY, Song WT, Lee SH, Hong JH, Lee CK, Kang SG. Neuroprotective effect of rice bran extract supplemented with ferulic acid in the rat model of ischemic brain injury. Animal Cells and Systems. 2014. 18: 93-100. https://doi.org/10.1080/19768354.2014.904249
  2. Carey F, Menashi S, Crawford N. Localization of cyclo-oxygenase and thromboxane synthase in human platelet intracellular membranes. Biochem J. 1982. 204: 847-851. https://doi.org/10.1042/bj2040847
  3. Cattaneo M, Tenconi PM, Lecchi A, Mannucci PM. In vitro effects of picotamide on human platelet aggregation, the release reaction and thromboxane $B_2$ production. Thromb Res. 1991. 62: 717-724. https://doi.org/10.1016/0049-3848(91)90375-7
  4. Clutton P, Folts JD, Freedman JE. Pharmacological control of platelet function. Pharmacol Res. 2001. 44: 255-264. https://doi.org/10.1006/phrs.2001.0861
  5. DeWitt DL, el-Harith EA, Kraemer SA, Andrews MJ, Yao EF, Amstrong RL, Smith WL. The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases. J Biol Chem. 1990. 265: 5192-5198.
  6. FitzGerald GA. Mechanisms of platelet activation: thromboxane $A_2$ as an amplifying signal for other agonists. Am J Cardiol. 1991. 68: 11B-15B. https://doi.org/10.1016/0002-9149(91)90379-Y
  7. Goufo P, Trindade H. Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, ${\gamma}$-oryzanol, and phytic acid. Food Sci Nutr. 2014. 2: 75-104. https://doi.org/10.1002/fsn3.86
  8. Gresele P, Deckymyn H, Nenci GG, Vermylen J. Thromboxane synthase inhibitors, thromboxane receptor antagonists and dual blockers in thrombotic disorders. Trends Pharmacol Sci. 1991. 12: 158-163. https://doi.org/10.1016/0165-6147(91)90533-X
  9. Hamberg M, Svensson J, Samuelsson B. Thromboxanes: a new group of biologically active compounds derived from prosta-glandin endoperoxides. Proc Natl Acad Sci USA. 1975. 72: 2994-2998. https://doi.org/10.1073/pnas.72.8.2994
  10. Hong JH, Kim JY, Baek SE, Ingkasupart P, Park HJ, Kang SG. Effects of rice bran extracts rermented with Lactobacillus plantarum on neuroprotection and cognitive improvement in a rat model of ischemic brain injury. Biomed Sci Lett. 2015. 21: 92-102. https://doi.org/10.15616/BSL.2015.21.2.92
  11. Hou Z, Qin R, Ren G. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. Japonica) on chronically alcohol-induced liver damage in rats. J Agric Food Chem. 2010. 58: 3191-3196. https://doi.org/10.1021/jf904407x
  12. Jang EK, Azzam JE, Dickinson NT, Davidson MM, Haslam RJ. Roles for both cyclic GMP and cyclic AMP in the inhibition of collagen-induced platelet aggregation by nitroprusside. Br J Haematol. 2002. 117: 664-675. https://doi.org/10.1046/j.1365-2141.2002.03479.x
  13. Jennings LK. Role of platelets in atherothrombosis. Am J Cardiol. 2009. 103(3 Suppl): 4A-10A. https://doi.org/10.1016/S0002-9149(08)02058-4
  14. Jun HI, Song GS, Yang EI, Youn Y, Kim YS. Antioxidant activities and phenolic compounds of pigmented rice bran extracts. J Food Sci. 2012. 77: C759-C764. https://doi.org/10.1111/j.1750-3841.2012.02763.x
  15. Kim HH, Hong JH, Ingkasupart P, Lee DH, Park HJ. Inhibitory effects of water extract from rice bran due to cAMP-dependent phosphorylation of VASP ($Ser^{157}$) on ADP-induced platelet aggregation. Biomed Sci Lett. 2014. 20: 129-138. https://doi.org/10.15616/BSL.2014.20.3.129
  16. Kim HH, Lee DH, Hong JH, Ingkasupart P, Nam GS, Ok WJ, Kim MJ, Yu YB, Kang HC, Park HJ. Inhibitory effects of rice bran water extract fermented Lactobacillus plantarum due to cAMP-dependent phosphorylation of VASP ($Ser^{157}$) on human platelet aggregation. Biomed Sci Lett. 2015. 21: 103-114. https://doi.org/10.15616/BSL.2015.21.2.103
  17. Lagarde M, Menashi S, Crawford N. Localisation of phospholipase $A_2$ and diglyceride lipase activities in human platelet intracellular membranes. FEBS Lett. 1981. 124: 23-26. https://doi.org/10.1016/0014-5793(81)80045-2
  18. Lewis GP, Watts IS. Prostaglandin endoperoxides, thromboxane $A_2$ and adenosine diphosphate in collagen-induced aggregation of rabbit platelets. Br J Pharmacol. 1982. 75: 623-631. https://doi.org/10.1111/j.1476-5381.1982.tb09183.x
  19. Li Z, Delaney MK, O'Brien KA, Du X. Signaling during platelet adhesion and activation. Arteroscler Thromb Vasc Biol. 2010. 30: 2341-2349. https://doi.org/10.1161/ATVBAHA.110.207522
  20. Malmsten C, Hamberg M, Svensson J, Samuelsson B. Physiological role of an endoperoxide in human platelets: hemostatic defect due to platelet cyclooxygenase deficiency. Proc Natl. Acad Sci USA. 1975. 72: 1446-1450. https://doi.org/10.1073/pnas.72.4.1446
  21. Moriyama T, Takamura H, Narita H, Tanaka K, Matsuura T, Kito M. Elevation of cytosolic free $Ca^{2+}$ is directly evoked by thromboxane $A_2$ in human platelets during activation with collagen. J Biochem. 1988. 103: 901-902. https://doi.org/10.1093/oxfordjournals.jbchem.a122383
  22. Ohkubo S, Nakahata N, Ohizumi Y. Thromboxane $A_2$-mediated shape change: independent of $G_q$-phospholipase C-$Ca^{2+}$ path-way in rabbit platelets. Br J Pharmacol. 1996. 117: 1095-1104. https://doi.org/10.1111/j.1476-5381.1996.tb16702.x
  23. Patrono C. Aspirin: New cardiovascular uses for an old drug. Am J Med. 2001. 110: 62S-65S. https://doi.org/10.1016/S0002-9343(01)00750-1
  24. Qureshi AA, Sami SA, Khan FA. Effects of stabilized rice bran, its soluble and fiber fractions on blood glucose levels and serum lipid parameters in humans with diabetes mellitus Types I and II. J Nutr Biochem. 2002. 13: 175-187. https://doi.org/10.1016/S0955-2863(01)00211-X
  25. Roth GJ, Stanford N, Majerus PW. Acetylation of prostaglandin synthase by aspirin. Proc Natl Acad Sci USA. 1975. 72: 3073-3076. https://doi.org/10.1073/pnas.72.8.3073
  26. Ruggeri ZM. Platelets in atherothrombosis. Nat Med. 2002. 8: 1227-1234. https://doi.org/10.1038/nm1102-1227
  27. Samuelsson B, Goldyne M, Granstrom E, Mamberg M, Hammarstrom S, Malmsten C. Prostaglandin and thromboxanes. Annu Rev Biochem. 1978. 47: 997-1029. https://doi.org/10.1146/annurev.bi.47.070178.005025
  28. Schwartz SM, Heinmark RL, Majesky MW. Developmental mechanisms underlying pathology of arteries. Physiol Rev. 1990. 70: 1177-1209. https://doi.org/10.1152/physrev.1990.70.4.1177
  29. Singleton VL, Rossi JA. Colorimetry of total phenolics with phos-phomolybdicphosphotungstic acid reagents. Am J Enol Vitic. 1965. 16: 144-158.
  30. Su CY, Shiao MS, Wang CT. Differential effects of ganodermic acid S on the thromboxane $A_2$-signaling pathways in human platelets. Biochem Pharmacol. 1999. 58: 587-595. https://doi.org/10.1016/S0006-2952(99)00136-7