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세포의 신호전달 과정에서 NO 항상성에 관한 융복합 연구

Convergence Studies of NO Homeostasis in Cellular Signalling

  • 오희균 (전남대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 도은영 (광주여자대학교 긴호학과) ;
  • 박해령 (광주여자대학교 교양교직과정부)
  • Oh, Hee-Kyun (Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Do, Eun-Young (Department of Nursing, Kwangju Women's University) ;
  • Park, Hae-Ryoung (Division of Liberal Arts & Teacher Training, Kwangju Women's University)
  • 투고 : 2015.10.24
  • 심사 : 2015.12.20
  • 발행 : 2015.12.28

초록

목향(Saussurea lappa)은 다양한 생리활성을 나타내는 성분들은 알려져 있으나 세포내에서 신호전달 체계에 미치는 영향에 대해서 자세히 알려진 바가 없다. 본 연구에서 목향의 에탄올 추출물이 Raw 264.7세포의 NO(nitric oxide) 항상성에 대한 논의를 하고자 한다. LPS(lipolysaccharide)로 유도한 후, NO(nitric oxide) 생성억제력과 IL-$1{\beta}$(interleukin-$1{\beta}$), TNF-${\alpha}$(tumour necrosis factor-${\alpha}$), iNOS와 COX-2를 전사수준(transcriptional level)에서 발현양상을 살펴보고자 한다. 목향의 에탄올 추출물은 RAW264.7 세포에서 LPS를 전처리한 후 유도되는 iNOS와 COX-2가 전사 수준에서 발현 억제와 pro-inflammatory cytokine인 TNF-${\alpha}$와 IL-$1{\beta}$의 발현을 효과적으로 저해함을 보였다. 본 연구 결과로 목향의 에탄올 추출물이 대식세포를 매개로 한 염증반응의 작용기전 연구에 있어서 NO(nitric oxide) 항상성에 영향이 있음을 융복합적 측면에서 고찰하였다. 향후 염증성 질환의 예방과 치료에 중요한 기초 자료로 제공하고 자 한다.

Saussurea lappa is known for a variety of physiological activities as a component but has not known to show the effect of the cellular signaling pathway. We investigated the anti-inflammatory effects by Saussurea lappa ethanol extract on the LPS(lipopolysaccharide) induced nitric oxide (NO) production by RAW 264.7 cell line. It shows the expressions of iNOS and COX-2 at the transcriptional level (RT-PCR). The Saussurea lappa ethanol extract showed transcriptional expression levels of pro-inflammatory cytokine TNF- and IL-$1{\beta}$ induced by LPS(lipolysaccharide) in RAW264.7 cell line. Saussurea lappa ethanol extract reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the RNA levels in a concentration-dependent manner. The finding that ethanol extract of Saussurea lappa has an influence on NO (nitric oxide) homeostasis in the study of the action mechanism on the macrophage-mediated inflammatory reaction was considered in terms of convergence. And it is to provide an important basis for the prevention and treatment of inflammatory diseases in the future.

키워드

참고문헌

  1. P. Batchelor, The changing epidemiology of oral diseases in the elderly, their growing importance for care and how they can be managed. Age Ageing, Vol. 44, No. 6, pp. 1064-1070, 2015. https://doi.org/10.1093/ageing/afv148
  2. D. J. Zuluaga, J. Ferreira, J. A. Montoya, T. Willumsen, Oral health in institutionalised elderly people in Oslo, Norway and its relationship with dependence and cognitive impairment. Gerodontology, Vol. 29, No. 2, pp. 420-426, 2012. https://doi.org/10.1111/j.1741-2358.2011.00490.x
  3. K. Sanjeev, S. Naganandini, R. Vasuda, N. Sachin, R. Rekha, M. S. Madhuniranjanswamy, Oral Hygiene Status of Institutionalised Dependent Elderly in India - a Cross-Sectional Survey. Can Geriatr J., Vol. 18, No. 2, pp. 51-56, 2015.
  4. F. Cindy, Iron Deficiency Anemia: A Guide to Oral Iron Supplements. Clinical Correlation, 2015.
  5. M. Harold, C. L. Marc, Oral Microbial Ecology and the Role of Salivary Immunoglobulin A. Microbiol. Mol. Biol. Rev. Vol. 62 No. 1 pp. 71-109, 1998.
  6. W. C. Nance, S. E. Dowd, D. Samarian, J. Chludzinski, J. Delli, J. Battista, A. H. Rickard, A high-throughput microfluidic dental plaque biofilm system to visualize and quantify the effect of antimicrobials. Antimicrob Chemother, Vol. 68, No. 11, pp. 2550-2560, J 2013. https://doi.org/10.1093/jac/dkt211
  7. D. M. Philip, Dental plaque as a biofilm and a microbial community-implications for health and disease. BMC Oral Health, Vol. 6(Suppl 1):S14, 2006. https://doi.org/10.1186/1472-6831-6-S1-S14
  8. J. Y. Choi, E. H. Choi, H. W. Jung, J. S. Oh, W. H. Lee, J. G. Lee, J. K. Son, Y. Kim, S. H. Lee, Melanogenesis inhibitory compounds from Saussureae Radix. Arch Pharm Res. Vol. 31, No. 3, pp. 294-299, 2008. https://doi.org/10.1007/s12272-001-1154-0
  9. N. H. Jung, J. Y. Ha, K. R. Min, F. Shibata, H. Nakagawa, S. S. Kang, I. M. Chang, Y. S. Kim, Reynosis from Saussurea lappa as inhibitor on CINC-1 induction in LPS-stimulated NRK-52E cells. Planta Med, Vol. 64, pp. 454-456, 1998. https://doi.org/10.1055/s-2006-957480
  10. Y. J. Jeon, H. S. Lee, S. Y. Yeon, J. H. Ko, K. M. An, S. W. Yu, J. H. Kang, B. Y. Hwang, T. Y. Kin. Inhibitory effect of dehydrocostuslactone isolated from Sussureae Radix on CDK2 activity. Kor J Pharmacogn 36: 97-101.2005.
  11. J. L. Ebersole, M. A. Taubmam, The protective nature of hostresponses in periodontal diseases. Periodontol 2000, Vol. 5, pp. 112-141, 1994. https://doi.org/10.1111/j.1600-0757.1994.tb00021.x
  12. K. S. Kornman, R. C. Page, M. S. Tonetti, The host response tothe microbial challenge in periodontitis: assembling the play-ers. Periodontol 2000, Vol. 14, pp. 33-53, 1997. https://doi.org/10.1111/j.1600-0757.1997.tb00191.x
  13. H. R. Park, S. J. Hong, Research on Natural Medicine for Wellness and Oral Health, The Journal of Digital Policy & Management, Vol. 13, No. 5, pp. 357-1363, 2015.
  14. T. Kirikae, T. Nitta, F. Kirikae, T. Suda, S. Kusumoto, N. Qureshi, M. Nakano, Lipopolysaccharides (LPS) of oral black pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different fromthat of Salmonella LPS. Infect Immun, Vol. 67, pp. 17-36, 1999.
  15. K. Seibert, Y. Zhang, K. Leahy, S. Hauser, J. Masferrer, W. Perkins, L. Lee, P. Ksakson, Pharmacological andbiochemical demonstration of the role of cyclooxygenase 2 ininflammation and pain. Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, pp. 12013-12017, 1994. https://doi.org/10.1073/pnas.91.25.12013
  16. M. Feldmann, F. M. Brennan, R. N. Maini, Role of cytokines in rheumatoid arthritis. Annual Review of Immunology, Vol. 14, pp. 397-440, 1996. https://doi.org/10.1146/annurev.immunol.14.1.397
  17. Y. C. Hseu, F. Y. Wu, J. J. Wu, J. Y. Chen, W. H. Chang, F. J. Lu, Y. C. Lai, H. L. Yang, Anti-inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway. Int Immunopharmacol, Vol. 5, pp. 1914-1925, 2005. https://doi.org/10.1016/j.intimp.2005.06.013
  18. B. Rudi, M. Alexander, Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells, and children. FEBS Letters, Vol. 275, No. 1-2, pp. 87-90, 1990. https://doi.org/10.1016/0014-5793(90)81445-T
  19. M. Takeshi, N. Toshio, H. Keiichi, S. Hiromichi, K. Ryuichi, S. Takao, Cyclosporin A Inhibits Nitric Oxide Synthase Induction in Vascular Smooth Muscle Cells. Hypertension, Vol. 25, pp. 764-768, 1995. https://doi.org/10.1161/01.HYP.25.4.764
  20. M. Cetkovic-Cvrlje, D. L. Eizirik, TNF and IFN ${\gamma}$ potentiate the deleterious effects of IL-$1{\beta}$ on mouse pancreatic islets mainly viageneration of nitric oxide. Cytokine, Vol. 6, pp. 399-406, 1994. https://doi.org/10.1016/1043-4666(94)90064-7