Childhood Kidney Diseases

Korean Society of Pediatric Nephrology (대한소아신장학회)
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Effects of Human Adipose-Derived Stem Cells in Regenerating the Damaged Renal Tubular Epithelial Cells in an Animal Model of Cisplatin-Induced Acute Kidney Injury

  • Kim, Saeyoon (Department of Pediatrics, College of Medicine, Yeungnam University) ;
  • Lee, Eung Bin (Department of Pediatrics, College of Medicine, Yeungnam University) ;
  • Song, In Hwan (Department of Anatomy, College of Medicine, Yeungnam University) ;
  • Kim, Yong Jin (Department of Pathology, College of Medicine, Yeungnam University) ;
  • Park, Hosun (Department of Microbiology, College of Medicine, Yeungnam University) ;
  • Kim, Yong Woon (Department of Physiology, College of Medicine, Yeungnam University) ;
  • Han, Gi Dong (School of Food Science and Food Service Industry, College of Natural Resources, Yeungnam University) ;
  • Kim, Kyung Gon (Department of Anatomy, College of Medicine, Yeungnam University) ;
  • Park, Yong Hoon (Department of Pediatrics, College of Medicine, Yeungnam University)
  • Received : 2015.09.20
  • Accepted : 2015.10.26
  • Published : 2015.10.30
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Abstract

Background: We conducted this experimental study to examine whether human adipose-derived stem cells (ADSCs) are effective in achieving a recovery of damaged renal tubular epithelial cells in an animal model of cisplatin-induced acute kidney injury using rats. Methods: To examine the in vitro effects of ADSCs in improving nephrotoxicity, we treated mouse renal tubular epithelial cells with both ADSCs and cisplatin mouse renal tubular epithelial cells. And we equally divided 30 male white Sprague-Dawley (SD) rats into the three groups: the control group (intraperitoneal injection of a sterile saline), the cisplatin group (intraperitoneal injection of cisplatin) and the ADSC group (intraperitoneal injection of cisplatin and the hADSC via the caudal vein). At five days after the treatment with cisplatin, serum levels of blood urine nitrogen (BUN) and creatinine were measured from each SD rat. We performed histopathologic examinations of tissue samples obtained from the kidney. Results: The degree of the expression of TNF-${\alpha}$ and that of Bcl-2 were significantly higher and lower respectively, in cisplatin group (P<0.05). Serum levels of BUN (P=0.027) and creatinine (P=0.02) were significantly higher in cisplatin group. On histopathologic examinations, there was a significant difference in the ratio of the renal injury between cisplatin group and ADSC group (P=0.002). Conclusion: The ADSCs might have a beneficial effect in regenerating the damaged renal tubular epithelial cells.

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References

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