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Physicochemical Treatment for the Reduction of Fusarium spp. Infested in Adlay (Coix lacryma-jobi L.) Seeds

율무 종자 오염 Fusarium 속 진균 저감화를 위한 이화학적 처리

  • An, Tae Jin (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Guk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Hur, Mok (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Yun Ji (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cha, Sun Woo (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Oh, Sang Keun (Department of Applied Biology, Chungnam National University)
  • 안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영국 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 허목 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이윤지 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 오상근 (충남대학교 농생물학과)
  • Received : 2015.08.17
  • Accepted : 2015.11.21
  • Published : 2015.12.30

Abstract

Background : The aim of the present study was to identify an effective physicochemical control method to reduce Fusarium species infestation in adlay (Coix lacryma-jobi L.) before and after harvesting. Methods and Results : We observed that prochloraz emusifiable concentrate and hexaconazol prochloraz emusifiable concentrate strongly inhibited the mycelial growth of 10 Fusarium species. Strong growth inhibitions and cell lysis were observed following treatment with 4% NaOCl solution. The total number of fungi detected were lower follwing treatment with thiophanatemethyl triflumizole wettable powder ($1.1{\times}10^4CFU/g$), hexaconazol prochloraz emulsifiable concentrate ($1.2{\times}10^4CFU/g$), carboxin thiram dustable powder ($1.6{\times}10^4CFU/g$) and prochloraz emulsifiable concentrate ($1.7{\times}10^4CFU/g$) than in the non-treated control ($7.7{\times}10^4CFU/g$). The reduction of Fusarium fungi varies with the concentration and soaking time of NaOCl solution. Fungal detection was not observed after soaking in NaOCl solution for 24 h and harmful effects were not observed for plant growth by NaOCl after soacking for 6 - 12 h. Conclusion : Soaking seed for 6 - 12 h in 4% NaOCl could be an effective method of disinfectant treatment for the control of Fusarium fungi in adlay seeds.

Keywords

References

  1. Chae KY. (2009). Quality characteristics of glutinous rice Dasik by the the addition of Job's tears flour. Korean Journal of Food Cookery Science. 25:1-7.
  2. Choi HJ, An TJ, Kim JI, Park SH, Kim DW, Ahn YS and Moon YS. (2014). Postharvest strategies for deoxynivalenol and zearalenone reduction in stored adlay(Coix lachryma-jobi L.) grains. Journal of Food Protection. 77:466-471. https://doi.org/10.4315/0362-028X.JFP-13-284
  3. Choi HW, Hong SK, Kim WG and Lee YK. (2011). Diversity and pathogenicity of Fusarium species associated with head blight of Job's tears. The Korean Journal of Mycology. 39:217-222. https://doi.org/10.4489/KJM.2010.39.3.217
  4. Chung YJ, Kim JK and Youn KS. (2006). Effect of roasting temperature on phycochemical properties of Job's tears(Coix lachryma-jobi L. var. ma-yeun) powder and extracts. Korean Journal of Food Preservation. 13:477-482.
  5. Jang JH, Yi ES, Choi BY, Kim IJ, Park JS, Kim SK and Kim HD. (2005). New variety "Johyun" of Coix lachryma-jobi var. mayeun Stapf with early maturity and short plant height. Korean Journal of Medicinal Crop Science. 13:122-125.
  6. Kim HK, Cho DW and Hahm YT. (2000). The effects of Coix Bran on lipid metabolism and glucose challenge in hyperlipidemic and diabetic rats. Journal of the Korean Society of Food Science and Nutrition. 29:140-146.
  7. Kim SK, Kim KW, Hong SS, Park EW, Yang JS and Kim YJ. (1997). Isolation and identification of Bipolaris coicis, causing leaf blight of Job's tears. The Korean Journal of Mycology. 25:291-296.
  8. Korean Society of Plant Pathology(KSPP). (2009). List of plant disease in Korea. Korean Society of Plant Pathology. Suwon, Korea. p.278-279.
  9. Lee JE, Suh MH, Lee HG and Yang CB. (2002). Characteristics of Job's tear gruel by various mixing ratio, particle size and soaking time of Job's tear and rice flour. Korean Journal of Food and Cookery Science. 18:193-199.
  10. Leslie JF, Plattner RD, Desjardins AE and Klittich CJR. (1992). Fumonisin B1 production by strains from different mating populations of Gibberrella fujikuroi(Fusarium section liseola). Phytopathology. 82:341-345. https://doi.org/10.1094/Phyto-82-341
  11. Martins ML and Martins HM. (2002). Influence of water activity, temperature and incubation time on the simultaneous production of deoxynivalenol and zearalenone in corn(Zea mays) by Fusarium graminearum. Food Chemistry. 79:315-318. https://doi.org/10.1016/S0308-8146(02)00147-4
  12. Ministry of Agriculture, Food and Rural Affair(MAFRA). (2013). Production record of cash crops. Ministry of Agriculture, Food and Rural Affair. Sejong, Korea. p.51.
  13. Numata M, Yamamoto A, Moribayashi A and Yamada H. (1994). Antitumor components isolated from the chinese herbal medicine Coix lachryma-jobi. Planta Medica. 60:356-359. https://doi.org/10.1055/s-2006-959500
  14. Park GS and Lee SJ. (1999). Effect of Job's tears powder and green tea powder on the characteristics of quality of bread. Journal of the Korean Society of Food Science and Nutrition. 28:1244-1250.
  15. Takahashi M, Konno C and Hikino H. (1986). Isolation and hypoglycemic activity of coixans A, B and C, glycans of Coix lachryma-jobi L. var. ma-yuen seeds. Planta Medica. 52:64-65. https://doi.org/10.1055/s-2007-969074
  16. Ukita T and Tanimura A. (1961). Studies on the anti-tumor component in the seeds of Coix lachryma-jobi L. var. mayuen( Roman) STAPF. I. Isolation and anti-tumor activity of coixenolide. Chemical and Pharmaceutical Bulletin. 9:43-46. https://doi.org/10.1248/cpb.9.43
  17. Yoshizawa T and Morooka N. (1973). Deoxynivalenol and its monoacetate: New mycotoxins from Fusarium roseum and mouldy barley. Agricultural and Biological Chemistry. 37:2993-2934.