Inhibitory Effect of Crude Extracts from a Brown Alga Sargassum siliquanstrum on 3T3-L1 Adipocyte Differentiation

꽈배기모자반 추출물이 3T3-L1 지방세포 분화에 미치는 영향

  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Lee, Jung Im (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Kwon, Myeong Sook (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Seo, Youngwan (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
  • 공창숙 (신라대학교 의생명과학대학 식품영양학과) ;
  • 이정임 (한국해양대학교 해양과학기술대학 해양생명과학부) ;
  • 권명숙 (신라대학교 의생명과학대학 식품영양학과) ;
  • 서영완 (한국해양대학교 해양과학기술대학 해양생명과학부)
  • Received : 2015.10.22
  • Accepted : 2015.11.28
  • Published : 2015.12.30


In this study, the potential capacity of the crude extract and its solvent fractions from S. siliquanstrum against adipocyte differentiation were evaluated in 3T3-L1 adipocytes. The anti-adipogenic effect of S. siliquanstrum was evidenced by the fact that its crude extract decreases the lipid accumulation of differentiating cells and the expression levels of crucial adipogenesis markers, peroxisome proliferator-activated receptor $(PPAR){\gamma}$ and CCAAT/enhancer-binding protein $(C/EBP){\alpha}$. All solvent fractions except the water fraction showed an observable decrease in lipid accumulation and $PPAR{\gamma}$ and $C/EBP{\alpha}$ expressions. In conclusion, these results suggest that S. siliquanstrum possesses obesity inhibiting components, which may possibly be used as a valuable anti-obesity agent for reducing the risk of obesity.



  1. Ali MS, Jahangir M, Saleen M, Pervez MK, Hameed S, Ahmad VU (2000) Metabolites of marine algae collected from Karachi-coasts of Arabian sea. Nature Pro Sci 6(2):61-65
  2. Cho SH, Kang SE, Cho JY, Kim AR, Park SM, Hong YK (2007) The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts. J Med Food 10(3):479-485
  3. Clarkson RW, Chen CM, Harrison S, Wells C, Muscat GE, Waters MJ (1995) Early responses of trans-activating factors to growth hormone in preadipocytes: differential regulation of CCAAT enhancer-binding protein-beta (C/EBP beta) and C/EBP delta. Mol Endocrinol 9(1):108-120
  4. Dawczynski C, Schubert R, Jahreis G (2007) Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem 103(3):891-899
  5. Green H, Kehinde O (1974) Sublines of mouse 3T3 cells that accumulate lipid. Cell 1(3):113-116
  6. Kang JW (1987) Marine botany. Daehan Book Publishing Co., Seoul, 497 p
  7. Kim AR, Song EJ, Kim MJ, Lee SY, Kim KBWR, Kim JH, Kim SJ, Hong YK, Park JG, Kim JH, Lee JW, Byun MW, Ahn DH (2008) Effects of gamma irradiation on antioxidant properties and physical characteristics of Sargassum siliquastrum water extract. J Korean Soc Food Sci Nutr 37(3):357-361
  8. Kim MJ, Song EJ, Kim KBWR, Lee CJ, Jung JY, Kwak JH, Choi MK, Kim DH, Sunwoo C, Choi JS, Choi HD, Ahn DH (2011) Effect of Sargassum fulvellum extracts on shelf-life and quality improvement of bread. J Korean Soc Food Sci Nutr 40(6):867-874
  9. Kong CS, Kim JA, Bak SS, Byun HG, Kim SK (2011) Anti-obesity effect of carboxymethyl chitin by AMPK and aquaporin-7 pathways in 3T3-L1 adipocytes. J Nutri Biochem 22(3):276-281
  10. Kong CS, Seo Y (2012) Anti-adipogenic activity of isohamnetin 3-O-${\beta}$-D-glucopyranoside from Salicornia herbacea. Immunopharm Immunot 34(6):907-911
  11. Lahaye M, Kaeffer B (1997) Seaweed dietary fibres: structure, physic-chemical and biological properties relevant to intestinal physiology. Sci Aliment 17(6):563-584
  12. Lee SY, Kim JH, Kim KBWR, Song EJ, Kim AR, Park SM, Han CS, Ahn DH (2007) Antimicrobial activities of medicinal herbs and seaweeds extracts against microorganisms isolated from the rice warehouses. J Korean Soc Food Sci Nutr 36(4):476-480
  13. Lee SY, Kim KBWR, Song EJ, Kim JH, Kim AR, Kim MJ, Moon JH, Kang HM, Lee HD, Hong YK, Ahn DH (2008) Effect of extracts from Sargassum siliquastrum on shelf-life and quality of bread. J Korean Soc Food Sci Nutr 37(4):490-496
  14. Numata A, Kanbara S, Takahashi C, Fujiki R, Yoneda M, Usami Y, Fujita E (1992) A cytotoxic principle of the brown alga Sargassum tortile and structures of chromenes. Phytochemistry 31(4):1209-1213
  15. Park JC, Choi JS, Song SH, Choi MR, Kim KY, Choi JW (1997) Hapatoprotective effect of extracts and phenolic compound from marine algae in bromobenzene-treated rats. Korean J Pharmacogn 28(4):239-246
  16. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, Spiegelman BM (2001) $C/EBP{\alpha}$ induces adipogenesis through PPAR: a unified pathway. Gene Dev 16(1):22-26
  17. Ruperez P (2002) Mineral content of edible marine seaweeds. Food Chem 79(1):23-26
  18. Sanchez-Machado DI, Lopez-Cervantes J, Lopez-Hernandez J, Paseiro-Losada P (2003) Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chem 85(3):439-444
  19. Schoonjans K, Peinado-Onsurbe J, Lefebvre AM, Heyman RA, Briggs M, Deeb S, Staels B, Auwerx J (1996) PPAR alpha and PPAR gamma activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene. EMBO J 15(19):5336-5348

Cited by

  1. before solvent extraction of antioxidant and antiobesity compounds pp.02682575, 2018,