한국어병학회지 (Journal of fish pathology)

  • 제28권3호
  • /
  • Pages.117-123
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  • 2015
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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멍게 물렁증의 원인충인 Azumiobodo hoyamushi에 대한 유기산의 살충효과 연구

Anti-protozoal effect of organic acids against Azumiobodo hoyamushi that causes soft tunic syndrome to Halocynthia roretzi

  • 이지훈 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박경일 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Lee, Ji Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Kyung Il (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 투고 : 2015.09.08
  • 심사 : 2015.12.10
  • 발행 : 2015.12.30
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초록

우리나라 멍게 양식의 발전으로 생산량이 점점 증가하였으나 최근 물렁증 (soft tunic syndrome)으로 멍게가 대량 폐사하여 경제적으로 많은 손실을 입고 있다. 이런 물렁증을 일으키는 원인체가 Azumiobodo hoyamushi라고 보고되었다. 하지만 이 원인충을 박멸할 수 있는 치료제 연구는 미비한 실정이다. 저자들에 의한 이전의 연구에서 기존의 산화제, 환원제, 소독제 등에서 물렁증에 대한 치료효과가 있다고 보고하였다. 본 연구에서는 기존의 약제보다 좀 더 친환경적인 유기산을 활용하여 in vitro 및 in vivo 시험을 통하여 연구하였다. In vitro 시험에서 12종의 유기산 중 8종이 $300{\mu}g/ml$에서 100% 원충에 대한 살충효과를 발휘하였다. In vivo에서의 유기산 침투효과를 평가하기 위해 pH의 변화를 측정한 결과, 유기산 처리 후 멍게 피막내 pH는 in vitro 에서 보다는 0.5~1 정도 높고 무처리 피막보다는 2.0 정도가 낮아 부분적으로는 침투가 일어남을 확인하였다. 본 연구를 토대로 친환경적인 유기산을 이용하여 물렁증의 치료에 활용할 수 있을 것이다.

Economic loss by soft tunic syndrome of edible ascidian, Halocynthia roretzi has become a serious problem. Recently, it has discovered that the cause of this syndrome is infection by a protozoan parasite Azumiobodo hoyamushi. However, only a few studies have been conducted to control this parasitic disease. In a previous research, non-specific disinfectants have been found to be effective in controling the causative parasite. In an attempt to eradicate this causative parasite, organic acids were tested in this study to evaluate their in vitro and in vivo efficacy. In vitro tests showed that 8 different organic acids used in this study were moderately or highly effective with protozoan-killing effects ($EC_{50}=153{\sim}275{\mu}g/ml$). Despite weak in vivo penetration of organic acids into the tunic tissues, treatment with high concentration reduced the mortality of ascidian caused by infection the parasite, indicating that we might be able to develop a disinfection method using environmentally-friendly organic acids.

키워드

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