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The Influence of Adjuvants on Herbicide Activity of Streptomyces scopuliridis KR-001

토양 방선균 Streptomyces scopuliridis KR-001 균주 배양액의 살초활성을 증가시키는 Adjuvant 탐색

  • Kim, Jae Deok (Eco - friendly and New Materials Research Center, Korea Research Institute of Chemical Technology) ;
  • Sin, Hoon Tak (Eco - friendly and New Materials Research Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Young Sook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ko, Young Kwan (Eco - friendly and New Materials Research Center, Korea Research Institute of Chemical Technology) ;
  • Cho, Nam Kyu (Korea Research Institute of Bioscience and Biotechnology) ;
  • Hwang, Ki Hwan (Korea Research Institute of Bioscience and Biotechnology) ;
  • Koo, Suk Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Jung Sup (Eco - friendly and New Materials Research Center, Korea Research Institute of Chemical Technology) ;
  • Park, Kee Woong (Department of Crop Science, Chungnam National University)
  • 김재덕 (한국화학연구원 친환경신물질연구센터) ;
  • 신훈탁 (한국화학연구원 친환경신물질연구센터) ;
  • 김영숙 ((주)목우연구소) ;
  • 고영관 (한국화학연구원 친환경신물질연구센터) ;
  • 조남규 ((주)목우연구소) ;
  • 황기환 ((주)목우연구소) ;
  • 구석진 ((주)목우연구소) ;
  • 최정섭 (한국화학연구원 친환경신물질연구센터) ;
  • 박기웅 (충남대학교 식물자원학과)
  • Received : 2015.11.30
  • Accepted : 2015.12.09
  • Published : 2015.12.30

Abstract

This study was conducted to investigate efficient adjuvants to increase herbicidal efficacy of metabolites from Streptpmyces scopuliridis KR-001. Commonly used 21 adjuvants mixed with the metabolites were applied to eight weed species (six grass weeds and two broadleaved weeds). Based on the visual evaluation, two adjuvants, LE7 (Polyoxyethylene lauryl ether) and EP4C (Sodium bis (2-ethylhexyl) sulfosuccinate), were selected as most efficient adjuvants to elevate herbicidal efficacy of the metabolites. Higher efficacy in the LE7 and EP4C was obtained when overall spray volume was $2,000L\;ha^{-1}(65{\mu}g\;a.i.\;ml^{-1})$ than $1,000L\;ha^{-1}(130{\mu}g\;a.i.\;ml^{-1})$. Field study demonstrated that $1,300{\mu}g\;ml^{-1}$ of metabolites from KR-001 applied with EP4C at concentration of $2{\mu}g\;ml^{-1}$ provided a highly effective post-emergence weed control which was almost equivalent to the glufosinate-ammonium at $540g\;a.i.\;ha^{-1}$. On the basis of these results, combination and multiple application methods could be developed to enhance herbicidal efficacy of metabolites from KR-001.

본 연구는 토양방선균 Streptomyces scopuliridis KR-001 대사물질의 살초활성을 증가시키는 adjuvant를 선발하기 위해 수행되었다. 작물보호제에서 보편적으로 사용하는 21종의 adjuvant를 8종(화본과잡초 6종, 광엽잡초 2종)의 피검식물에 처리하여 살초활성이 상대적으로 증가된 LE7[Polyoxyethylene lauryl ether]과 EP4C [Sodium bis (2-ethylhexyl) sulfosuccinate]를 선발하였다. 선발된 2종의 adjuvant를 KR-001 대사물질과 함께 8종(화본과잡초 4종, 광엽잡초 4종)의 피검식물을 대상으로 살포한 결과 $2,000L\;ha^{-1}(65{\mu}g\;a.i.\;ml^{-1})$ 처리가 $1,000L\;ha^{-1}(130{\mu}g\;a.i.\;ml^{-1})$ 처리보다 살초활성이 더 높게 나타났다. 또한 6종(화본과 잡초 3종, 광엽잡초 3종)의 피검식물에 대하여 포장 실증평가를 수행한 결과 $1,300{\mu}g\;ml^{-1}$의 KR-001 대사물질에 $2{\mu}g\;ml^{-1}$ 농도로 EP4C를 첨가하여 $2,000L\;ha^{-1}$ 처리량으로 살포시 대조약제인 glufosinate-ammonium ($540g\;a.i.\;ha^{-1}$)과 대등한 약효를 보였다. 향후 선발된 2종의 adjuvant를 대상으로 복합 및 다중처리와 잡초의 생육 정도에 따른 처리시기 조절 등으로 보다 효과적인 약효증진 기술을 개발하고자 한다.

Keywords

References

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