Journal of Animal Science and Technology

  • 제57권4호
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  • Pages.16.1-16.7
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  • 2015
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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DOI QR코드

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Molecular cloning and characterization of Izumo1 gene from bovine testis

  • Kim, Ekyune (College of Pharmacy, Catholic University of Daegu)
  • 투고 : 2014.12.03
  • 심사 : 2015.03.19
  • 발행 : 2015.04.30
⟨ 이전 논문 다음 논문 ⟩

초록

A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues indicated that bIZUMO1 was specifically expressed in the testis and sperm, Furthermore, the result of our biotinylation assay from ejaculated bovine sperm strongly suggest the assumption that bIZUMO1 is localized on the cell surface. These data imply the potential role of bovine IZUMO1 in mammalian fertilization.

키워드

과제정보

연구 과제 주관 기관 : Catholic University of Daegu

참고문헌

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  12. Kim E, Lee JW, Baek DC, Lee SR, Kim MS, Kim SH, et al. Processing and subcellular localization of ADAM2 in the Macaca fascicularis testis and sperm. Anim Reprod Sci. 2010;117:155-9. https://doi.org/10.1016/j.anireprosci.2009.04.002
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  14. Kim E, Kim JS, Lee Y, Song BS, Sim BW, Kim SU, et al. Molecular cloning, characterization of porcine IZUMO1, an IgSF family member. Reprod Domest Anim. 2013;48:90-7. https://doi.org/10.1111/j.1439-0531.2012.02037.x
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