The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR

  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Yea, Mi-Chi (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Back, Chang-Gi (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Choi, Kwang-Shik (College of Natural Sciences, Kyungpook National University) ;
  • Kang, In-Kyu (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Su-Heon (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • 투고 : 2014.05.07
  • 심사 : 2014.08.12
  • 발행 : 2014.12.01
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초록

The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield.

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참고문헌

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