DOI QR코드

DOI QR Code

Preparation of Lysine-Coated Magnetic Fe2O3 Nanoparticles and Influence on Viability of A549 Lung Cancer Cells

  • Ma, Yu-Hua (Department of Clinical Laboratory Medicine, Linyi People's Hospital) ;
  • Peng, Hai-Ying (Department of Clinical Laboratory Medicine, Linyi People's Hospital) ;
  • Yang, Rui-Xia (Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University) ;
  • Ni, Fang (Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University)
  • 발행 : 2014.11.06

초록

Objective: To explore the effect of lysine-coated oxide magnetic nanoparticles (Lys@MNPs) on viability and apoptosis of A549 lung cancer cells. Methods: Transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and Zeta potentiometric analyzer were employed to characterize Lys@MNPs. Then Lys@MNPs and lung cancer A549 cells were co-cultured to study the effect of Lys@MNPs on cell viability and apoptosis. The pathway of Lys@MNPs entering A549 cells was detected by TEM and cell imaging by 1.5 T MRI. Results: Lys@MNPs were 10.2 nm in grain diameter, characterized by small size, positive charge, and superparamagnetism. Under low-dose concentration of Lys@MNPs (< $40{\mu}g/mL$), the survival rate of A549 cells was decreased but remained higher than 95% while under high-dose concentration ($100{\mu}g/mL$), the survival ratewas still higher than 80%, which suggested Lys@MNPs had limited influence on the viability of A549 cells, with good biocompatibility and and no induction of apoptosis. Moreover, high affinity for cytomembranes, was demonstrated presenting good imaging effects. Conclusion: Lys@MNPs can be regarded as a good MRI negative contrast agents, with promising prospects in biomedicine.

키워드

참고문헌

  1. Bakhru SH, Altiok E, Highley C, et al (2012). Enhanced cellular uptake and long-term retention of chitosan-modified iron-oxide nanoparticles for MRI-based cell tracking. Int J Nanomed, 7, 4613-23.
  2. Basuki JS, Duong HTT, Macmillan A, et al (2013). Polymergrafted, nonfouling, magnetic nanoparticles designed to selectively store and release molecules via ionic interactions. macromolecules, 46, 7043-54. https://doi.org/10.1021/ma401171d
  3. Barick KC, Singh S, Bahadur D, et al (2014). Carboxyl decorated Fe3O4 nanoparticles for MRI diagnosis and localized hyperthermia. J Colloid Interface Sci, 418, 120-5. https://doi.org/10.1016/j.jcis.2013.11.076
  4. Bogart LK, Pourroy G, Murphy CJ, et al (2014). Nanoparticles for imaging, sensing, and therapeutic intervention. Acs Nano, 8, 3107-22. https://doi.org/10.1021/nn500962q
  5. Ding MM, Zeng X, He XL, et al (2014). Cell internalizable and intracellularly degradable cationic polyurethane micelles as a potential platform for efficient imaging and drug delivery. Biomacromolecules, 15, 2896-906. https://doi.org/10.1021/bm500506v
  6. Fang C, Kievit FM, Veiseh O, et al (2012). Fabrication of magnetic nanoparticles with controllable drug loading and release through a simple assembly approach. J Control Release, 162, 233-41. https://doi.org/10.1016/j.jconrel.2012.06.028
  7. Gupta AK, Gupta M (2005). Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles. Biomaterials, 26, 1565-73. https://doi.org/10.1016/j.biomaterials.2004.05.022
  8. Ge YQ, Zhang Y, Xia JG, et al (2009). Effect of surface charge and agglomerate degree of magnetic iron oxide nanoparticles on KB cellular uptake in vitro. Colloid Surface B Biointerfaces, 73, 294-301. https://doi.org/10.1016/j.colsurfb.2009.05.031
  9. Huang J, Wang LY, Lin R, et al (2013). Casein-coated iron oxide nanoparticles for high MRI contrast enhancement and efficient cell targeting. ACS Appl Mater Interfaces, 5, 4632-9. https://doi.org/10.1021/am400713j
  10. Huang G, Chen HB, Dong Y, et al (2013). Superparamagnetic iron oxide nanoparticles: amplifying ROS stress to improve anticancer drug efficacy. Theranostics, 3, 116-26. https://doi.org/10.7150/thno.5411
  11. Huang XE, Tian GY, Cao J, et al (2013). Pemetrexed as a component of first-, second- and third- line chemotherapy in treating patients with metastatic lung adenocarcinoma. Asian Pac J Cancer Prev, 14, 6663-7. https://doi.org/10.7314/APJCP.2013.14.11.6663
  12. Larsen EK, Nielsen T, Wittenborn T, et al (2012). Accumulation of magnetic iron oxide nanoparticles coated with variably sized polyethylene glycol in murine tumors. Nanoscale, 4, 2352-61. https://doi.org/10.1039/c2nr11554a
  13. Lee N, Choi Y, Lee Y, et al (2012). Water-dispersible ferrimagnetic iron oxide nanocubes with extremely high r (2) relaxivity for highly sensitive in vivo MRI of tumors. Nano Lett, 12, 3127-31. https://doi.org/10.1021/nl3010308
  14. Liang AL, Zhang TT, Zhou N, et al (2013). Fused polypeptide with DEF induces apoptosis of lung adenocarcinoma cells. Asian Pac J Cancer Prev, 14, 7339-44. https://doi.org/10.7314/APJCP.2013.14.12.7339
  15. Ma M, Zhang Y, Yu W, et al (2003). Preparation and characterization of magnetite nanoparticles coated by amino silane. Colloids Surf A Physicochem Eng Asp, 212, 219-26. https://doi.org/10.1016/S0927-7757(02)00305-9
  16. Rosen JE, Chan L, Shieh DB, et al (2012). Iron oxide nanoparticles for targeted cancer imaging and diagnostics. Nanomedicine, 8, 275-90. https://doi.org/10.1016/j.nano.2011.08.017
  17. Riaz S, Bashir M, Naseem S (2014). Iron oxide nanoparticles prepared by modified co-precipitation method. Ieee T Magn, 50.
  18. Sterenczak KA, Meier M, Glage S, et al (2012). Longitudinal MRI contrast enhanced monitoring of early tumour development with manganese chloride ($MnCl_2$) and superparamagnetic iron oxide nanoparticles (SPIOs) in a CT1258 based in vivo model of prostate cancer. BMC Cancer, 12, 284. https://doi.org/10.1186/1471-2407-12-284
  19. Singh A, Sahoo SK (2014). Magnetic nanoparticles: a novel platform for cancer theranostics. Drug Discov Today, 19, 474-81. https://doi.org/10.1016/j.drudis.2013.10.005
  20. Sun SH (2014). Chemical synthesis of monodisperse magnetic nanoparticles for sensitive cancer detection. J Inorg Organomet, P 24, 33-8. https://doi.org/10.1007/s10904-013-9975-x
  21. Wang YL, Li B, Xu F, et al (2012). In vitro cell uptake of biocompatible magnetite/chitosan nanoparticles with high magnetization: a single-step synthesis approach for in-situmodified magnetite by amino groups of chitosan. J Biomater Sci Polym Ed, 23, 843-60. https://doi.org/10.1163/092050611X562166
  22. Weis C, Blank F, West A, et al (2014). Labeling of cancer cells with magnetic nanoparticles for magnetic resonance imaging. Magn Reson Med, 71, 1896-905. https://doi.org/10.1002/mrm.24832
  23. Wabler M, Zhu WL, Hedayati M, et al (2014). Magnetic resonance imaging contrast of iron oxide nanoparticles developed for hyperthermia is dominated by iron content. Int J Hyperthermia, 30, 192-200. https://doi.org/10.3109/02656736.2014.913321

피인용 문헌

  1. Skin Cancer and Its Treatment: Novel Treatment Approaches with Emphasis on Nanotechnology vol.2017, pp.1687-4129, 2017, https://doi.org/10.1155/2017/2606271
  2. Microwave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell cultures pp.2190-5517, 2019, https://doi.org/10.1007/s13204-019-00962-1