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Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation

  • Seo, Hong Joo (Department of Thoracic and Cardiovascular Surgery, Chosun University Hospital) ;
  • Choi, Sang Joon (Department of Obstetrics and Gynecology, Chosun University Hospital) ;
  • Lee, Jung-Hee (Department of Cellular and Molecular Medicine, Chosun University School of Medicine)
  • Received : 2014.10.14
  • Accepted : 2014.11.04
  • Published : 2014.11.30

Abstract

Paraquat has been suggested to induce apoptosis by generation of reactive oxygen species (ROS). However, little is known about the mechanism of paraquat-induced apoptosis. Here, we demonstrate that extracellular signal-regulated protein kinase (ERK) is required for paraquat-induced apoptosis in NIH3T3 cells. Paraquat treatment resulted in activation of ERK, and U0126, inhibitors of the MEK/ERK signaling pathway, prevented apoptosis. Moreover, paraquat-induced apoptosis was associated with cytochrome C release, which could be prevented by treatment with the MEK inhibitors. Taken together, our findings suggest that ERK activation plays an active role in mediating paraquat-induced apoptosis of NIH3T3 cells.

Keywords

Acknowledgement

Supported by : Ministry of Education, Science and Technology

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