한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제29권3호
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  • Pages.221-228
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  • 2014
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
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Identification of Niche Conditions Supporting Short-term Culture of Spermatogonial Stem Cells Derived from Porcine Neonatal Testis

  • Park, Min Hee (Department of Animal Life Science, Kangwon National University) ;
  • Park, Ji Eun (Department of Animal Life Science, Kangwon National University) ;
  • Kim, Min Seong (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Kwon Young (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Yun, Jung Im (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Choi, Jung Hoon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Lee, Eunsong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Lee, Seung Tae (Department of Animal Life Science, Kangwon National University)
  • 투고 : 2014.08.19
  • 심사 : 2014.09.22
  • 발행 : 2014.09.30
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초록

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of $4{\times}10^5$ pSSCs on mitotically in activated $2{\times}10^5$ STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

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참고문헌

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