Korean Journal of Radiology

  • 제15권5호
  • /
  • Pages.591-604
  • /
  • 2014
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  • 1229-6929(pISSN)
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  • 2005-8330(eISSN)
대한영상의학회 (The Korean Society of Radiology)
권호 표지
DOI QR코드

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Intratumoral Heterogeneity of Breast Cancer Xenograft Models: Texture Analysis of Diffusion-Weighted MR Imaging

  • Yun, Bo La (Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Cho, Nariya (Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Li, Mulan (Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Jang, Min Hye (Department of Pathology, Seoul National University Bundang Hospital) ;
  • Park, So Yeon (Department of Pathology, Seoul National University Bundang Hospital) ;
  • Kang, Ho Chul (Department of Computer Science and Engineering, Seoul National University) ;
  • Kim, Bohyoung (Department of Radiology, Seoul National University Bundang Hospital) ;
  • Song, In Chan (Department of Radiology, Seoul National University Bundang Hospital) ;
  • Moon, Woo Kyung (Department of Radiology, Seoul National University Bundang Hospital)
  • 투고 : 2014.03.20
  • 심사 : 2014.06.07
  • 발행 : 2014.09.01
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: To investigate whether there is a relationship between texture analysis parameters of apparent diffusion coefficient (ADC) maps and histopathologic features of MCF-7 and MDA-MB-231 xenograft models. Materials and Methods: MCF-7 estradiol (+), MCF-7 estradiol (-), and MDA-MB-231 xenograft models were made with approval of the animal care committee. Twelve tumors of MCF-7 estradiol (+), 9 tumors of MCF-7 estradiol (-), and 6 tumors in MDA-MB-231 were included. Diffusion-weighted MR images were obtained on a 9.4-T system. An analysis of the first and second order texture analysis of ADC maps was performed. The texture analysis parameters and histopathologic features were compared among these groups by the analysis of variance test. Correlations between texture parameters and histopathologic features were analyzed. We also evaluated the intraobserver agreement in assessing the texture parameters. Results: MCF-7 estradiol (+) showed a higher standard deviation, maximum, skewness, and kurtosis of ADC values than MCF-7 estradiol (-) and MDA-MB-231 (p < 0.01 for all). The contrast of the MCF-7 groups was higher than that of the MDA-MB-231 (p = 0.004). The correlation (COR) of the texture analysis of MCF-7 groups was lower than that of MDA-MB-231 (p < 0.001). The histopathologic analysis showed that $Ki-67_{mean}$ and $Ki-67_{diff}$ of MCF-7 estradiol (+) were higher than that of MCF-7 estradiol (-) or MDA-MB-231 (p < 0.05). The microvessel density $(MVD)_{mean}$ and $MVD_{diff}$ of MDA-MB-231 were higher than those of MCF-7 groups (p < 0.001). A diffuse-multifocal necrosis was more frequently found in MDA-MB-231 (p < 0.001). The proportion of necrosis moderately correlated with the contrast (r = -0.438, p = 0.022) and strongly with COR (r = 0.540, p = 0.004). Standard deviation (r = 0.622, r = 0.437), skewness (r = 0.404, r = 0.484), and kurtosis (r = 0.408, r = 0.452) correlated with $Ki-67_{mean}$ and $Ki-67_{diff}$ (p < 0.05 for all). COR moderately correlated with $Ki-67_{diff}$ (r = -0.388, p = 0.045). Skewness (r = -0.643, r = -0.464), kurtosis (r = -0.581, r = -0.389), contrast (r = -0.473, r = -0.549) and COR (r = 0.588, r = 0.580) correlated with $MVD_{mean}$ and $MVD_{diff}$ (p < 0.05 for all). Conclusion: The texture analysis of ADC maps may help to determine the intratumoral spatial heterogeneity of necrosis patterns, amount of cellular proliferation and the vascularity in MCF-7 and MDA-MB-231 xenograft breast cancer models.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF), Seoul National University Hospital

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