디지털융복합연구 (Journal of Digital Convergence)

  • 제12권10호
  • /
  • Pages.577-585
  • /
  • 2014
  • /
  • 2713-6434(pISSN)
  • /
  • 2713-6442(eISSN)
한국디지털정책학회 (The Society of Digital Policy and Management)
권호 표지
DOI QR코드

DOI QR Code

OVA로 유도된 천식생쥐 모델에서 레몬 오일의 항천식 및 항염증 효과

Anti-asthma and anti-inflammation effects of lemon oil in OVA-induced allergic asthma mouse model

  • 최국기 (대전대학교 보건스포츠 대학원 대체의학과) ;
  • 정규진 (충남대학교 약학대학원 약학과) ;
  • 정광조 (대전대학교 뷰티건강관리학과)
  • Choi, Gook-Gi (Dept. of Alternative Medicinet Graduate School of Health & Sports, Daejeon University) ;
  • Chung, Kyu-Jin (College of Pharmacy, Chungnam National University) ;
  • Cheong, Kwang-Jo (Dept. of Beauty Health Care, Daejon University)
  • 투고 : 2014.08.05
  • 심사 : 2014.10.20
  • 발행 : 2014.10.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

레몬 오일의 향장효과와 각성효과는 미용분야와 심리치료분야에서 폭넓게 증명되어 왔다. 그러나 레몬 오일의 항천식과 항염증에 대한 고유한 효과에 대해 입증된 바는 드물다. 이 논문은 OVA로 유도된 천식 생쥐 모델에서 항천식 및 항염증 효과에 대해 증명하고자 수행되었다. OVA로 유도된 천식 생쥐 모델을 정상군, 대조군, 양성대조군 그리고 실험군으로 구성된 4집단으로 분류하였다. 0.3%의 레몬오일을 실험군에 3주간, 1주에 3회, 1회 30분간 흡입시켰다. 최초 실험일로부터 6주 후 기도 과민성의 정도, 혈액학적 변화, 혈청 내 사이토카인의 변화, 기도조직의 변화를 평가하였다. 실험 결과 레몬 오일은 효과적으로 기도과민성 억제 및 호산구의 세포증식 억제, IgE의 생성 억제를 통하여 기도의 염증 반응 및 과민반응을 유의성 있게 억제하는 것으로 확인되었다.

The cosmetic and stimulating effects of lemon oil have been widely proven in the fields of beauty therapy and psychology therapy. But the lemon oil's proper effects on the anti asthma and the anti inflammation have not rarely been verified. This study was conducted to find out the lemon oil's effects on the anti asthma and anti inflammation in the OVA-induced allergic asthma mice model. OVA-induced allergic asthma mice were divided into 4 groups consisting of normal group, control group, positive control group and experimental group. 0.3% of lemon oil was nebulized to experimental group for 3 weeks on a basis of 3 times per week and 30 min each time. After 6 weeks from the initial experiment, the degree of hyper activeness of respiratory system, the hematological change, the amount of the cytokein in serum, the change of airway organization were evaluated. The results showed that lemon oil controled effectively the hyper activeness of respiratory system and restricted the multiplication of cells in acidophil in terms of statistical significance. It also turned out that lemon oil restricted effectively the infection of airway reaction and the hyper sensitiveness of respiratory system by controlling the creation of histamine(cytokein) and IgE.

키워드

참고문헌

  1. Tai-yong Hong, The effect of anti-IgE antibody on airway inflammation in a mouse model of chronic asthma. M.S dissertation, Catholic University. 2009.
  2. Kirby, J. G. Hargreave, F. E. Gleich, G. J. O'Byme, P. M., Bronchoalveolar cell progiles of asthmatic and nonasthmatic subjects. Am Rev Respir Dis, Vol. 136, pp. 379-83. 1987. https://doi.org/10.1164/ajrccm/136.2.379
  3. Herd, C. M. Donigi-Gale, D. Shoupe, T. S. Kilfeather S. A. Okiji, S. A. Page, C. P., Effect of PF 10040 on PAF-induced airway responses in neonatally immunized rabbits. Br J.Pharmaco-l, Vol. 111, No. 1, pp. 7-12. 1994. https://doi.org/10.1111/j.1476-5381.1994.tb14016.x
  4. Yang-keun Rhee, Eosinophil in Asthma. J.Tube-rcu losis and respiratory diseases, Vol. 46, No 1, pp. 5-6, 1999. https://doi.org/10.4046/trd.1999.46.1.5
  5. Heusser, C. Jardieu, P., Therapeutic potential of anti-IgE antibadies. Curr Oinion in Immunology Vol. 9, p. 805. 1997. https://doi.org/10.1016/S0952-7915(97)80182-3
  6. Terada, N. Konno, A. Togawak., Biochemical preperties of eosinophils and their perferencial accumulation mechanism in nasal allergy. J Allergy Clin Immunol, Vol. 94, pp. 629-42. 1994. https://doi.org/10.1016/0091-6749(94)90140-6
  7. Jun-hyuk Lee, Association between CCR3 gene polymorphisms and eosinophilia in Korean with asthma. Ph.D. dissertation, Soonchunhyang University. 2007.
  8. Justice, J. P, Borchers, M. T. Crosby, R. Hines,E. M. Shen, H. H. Ochkur, S. I. McGarry, M.P. Lee, N. A. Lee, J., J. Ablation of eosinophils leads to a reduction of allergen-induced pulmonary path-logy. Am J Physiol Lung Cell Mol Physiol, Vol. 284, No. 1, pp. 169-78. 2003. https://doi.org/10.1152/ajplung.00260.2002
  9. Liu, Y. J,. Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity. Cell, Vol, 106, pp. 259-62. 2001. https://doi.org/10.1016/S0092-8674(01)00456-1
  10. Hye-Youn Lee, The effect of nebulized Frankin-cense essential oil in an OVA-induced allergic asthm mouse model. Ph.D.dissertation, KonKuk University. 2009.
  11. Hyun-hee Kim, Human Basophil. J.Pediatr Allergy Respir Dis(Korea),Vol. 12, No.12, pp. 71-81, 2002.
  12. Hyun-hee Kim, The role of human basophils in all ergic inflammation. J. Allergy Asthma Respir Dis(Korea), Vol. 22, No. 3, pp.507-513, 2002.
  13. Hill, S., Distribution, properties, and functional chaacteristics of three classes of histamine receptor. Pharm Rev, Vol. 42, pp. 45-82, 1990.
  14. Thomas, P. S. Yates, D. H. Barnes, P. J., TNT-inc reases airway responsiveness and sputum neurtrophilia in normal human subjects. Am J Respir CritCare Med, Vol. 152, pp. 76-80, 1995. https://doi.org/10.1164/ajrccm.152.1.7599866
  15. Bachereau, J. Steinman, R. M., Dendritic cells and the control of immunity. Nature, Vol.392, pp.245-52, 1998. https://doi.org/10.1038/32588
  16. Dong-ju Kim, The comparative study on the efficacy of Leukotriene receptor antagonist and antihistamine in combination and nasal deconge-stant and antihistamine in combination in allergicrhinitis, M.S dissertation, Gyeongsang National University, 2004.
  17. Skoner, D. P. Page, R. Asman, B. Cillen, B. Fireman, P., Plasma elevation of histamine and al prostagladin metabolite in acute asthma. Am Rev respir Dis, Vol. 137, pp. 1009-14, 1988. https://doi.org/10.1164/ajrccm/137.5.1009
  18. Ishizaka, K. Ishizaka T. Hombrook, M. M., Physico chemical properties of reaginic antibody. V. Correla tion of reaginic activity wth gamma-E-globulin antibody. J Immunol, Vol. 97, pp. 840-53, 1996.