KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant and Nitric Oxide Inhibitory Activities of Pigments from Chionoecetes japonicas Rathbun

홍게 (Chionoecetes japonicas Rathbun) 껍질 색소의 항산화 활성 및 Nitric Oxide 생성억제 효과

  • Park, Byungju (Busanil Science High School) ;
  • Baek, Seung Oh (Division of Marine Environment and Bioscience, Korea Maritime and Ocean University) ;
  • Song, Young-Sun (Center of Smart Foods and Drugs and Food Science Institute, Inje University) ;
  • Seo, Youngwan (Division of Marine Environment and Bioscience, Korea Maritime and Ocean University)
  • 박병주 (부산일과학고등학교) ;
  • 백승오 (한국해양대학교 해양환경생명과학부) ;
  • 송영선 (인제대학교 식의약생명공학과) ;
  • 서영완 (한국해양대학교 해양환경생명과학부)
  • Received : 2014.09.12
  • Accepted : 2014.10.13
  • Published : 2014.10.30
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Abstract

In the present study, antioxidant activities of two crude pigments (acetone and MeOH) and their solvent fractions (n-hexane, 85% aq.MeOH, n-BuOH, and water fractions) from red crab shell were evaluated by measuring 1,1-diphenyl-2-picryl hydrazyl (DPPH), peroxynitrites, and degree of production of reactive oxygen species (ROS) in HT 1080 cells as well as the extent of oxidative damage of genomic DNA purified from HT 1080 cells. From comparative analysis, 85% aq.MeOH fraction showed the strongest scavenging effect on both peroxynitrite in vitro and intracellular ROS in HT 1080 cells. Protective activities of these samples against hydroxyl radical-mediated genomic DNA damage were also investigated. 85% aq.MeOH and n-BuOH fractions significantly inhibited oxidative damage of purified genomic DNA. On the other hand, we investigated their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. All samples significantly reduced NO production. Among the samples, n-hexane and water solvent fractions most effectively inhibited NO.

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References

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