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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Uptake of Fibroin Microspheres by 3T3 Cells

3T3 세포에 의한 피브로인 마이크로스피어의 흡수

  • Lee, Jin Sil (Department of Biological Engineering and Technology, Kangwon National University) ;
  • Go, Nam Kyung (Scripps Korea Antibody Institute) ;
  • Lee, Shin Young (Department of Biological Engineering and Technology, Kangwon National University) ;
  • Hur, Won (Department of Biological Engineering and Technology, Kangwon National University)
  • 이진실 (강원대학교 생물공학과) ;
  • 고남경 ((재)스크립스코리아항체연구원) ;
  • 이신영 (강원대학교 생물공학과) ;
  • 허원 (강원대학교 생물공학과)
  • Received : 2014.03.05
  • Accepted : 2014.06.24
  • Published : 2014.10.30
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Abstract

Vehicle toxicity is one of the main obstacles for intracellular delivery of bioactive compounds. Silk fibroin is a natural polymer proven to have high biocompatibility since being used as suture material. In this report, fibroin microspheres were prepared without any chemical modification or cross-linking not to affect its biocompatibility. The microspheres were taken up by more than 90% of 3T3 cells. Cellular uptake continued after medium replenishment with a different-colored fluorescent microsphere, suggesting that simultaneous ingestion and exocytosis occurred. Cellular uptake of fibroin microspheres did not affect cell viability. Intracellular trafficking of the microspheres using lysosome-specific fluorescent dye revealed that fibroin microspheres were localized both in the cytoplasm and in the lysosome. Accordingly, fibroin microspheres can be a potential vehicle for intracytoplasmic delivery of large cargos, such as mixtures of proteins, nutrients or artificial organelles.

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References

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