Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Anti-allergic Effects of Samhwangsasim-tang ($S{\bar{a}}nhu{\acute{a}}ngxi{\grave{e}}x{\bar{i}}nt{\bar{a}}ng$) on Ovalbumin-induced Allergic Model in Mice

삼황사심탕(三黃瀉心湯)이 난황 알부민으로 유도된 알레르기 Mouse모델에서 항알레르기 효과

  • Choi, Chong-Hwan (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Keum, Seon-Oh (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Lee, Se-Won (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Kim, Il-Hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Lee, Ha-Il (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Song, Yung-Sun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University)
  • 최종환 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 금선오 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이세원 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 김일현 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이하일 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Received : 2014.06.18
  • Accepted : 2014.07.05
  • Published : 2014.07.31
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Abstract

Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-${\alpha}$ and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-${\gamma}$, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients.

Keywords

Acknowledgement

Supported by : 원광대학교

References

  1. 염석영. 임상알레르기학. 서울:여문각. 1984:10-9.
  2. 문희범. 알레르기질환의 임상과 실제. 서울:여문각. 1995: 2-11, 170-85.
  3. Metcalfe DD, Kaliner M, Donlon MA. The mast cell. Crit Rev Immunol. 1981;3:23.
  4. 강병수. 한방임상 알레르기. 성남:성보사. 1988:10, 19, 22-3, 189-210.
  5. 全宰弘, 金賢兒, 姜允皓. 接觸性皮膚炎에 關한文獻的考察. 韓方眼耳鼻咽喉皮膚科學會誌. 1997;10(1):263-83.
  6. Kim JB. A treatise on Essential prescriptions of the Golden coffer (Gum-Gye-Yo-Rak-Byun-Suk). Hanyunmoonhwasa. 2003:449-50.
  7. Oatsuka, K. Practical treatment of herbal medicine for various symptoms. Tokyo, Japan:Nanjantou. 1963:17, 453, 653, 679.
  8. Chung KH, Roh JH, Kim LD, et al. The effects of Samhwangsasim-tang extracts on ambulatory blood pressure in mild hypertensive patients with stroke. Journal of Korean Oriental Chronic Disease. 2002;8(1):41-52.
  9. Kim YJ, Lee MJ, Park JW, Kim JK, Choi DY, Kim CH. Antioxidant Activity of Water-Extract from Coptis chinensis Franch, J Life Sci. 2000;10(3):241-6.
  10. Lee JK, Park SJ, Kim SC, Ji SY. Coptidis Rhizoma Extract induces Apoptotic Cell Death in YD-10B Cell. J Korean Orient Med Ophthalmol Otolaryngol Dermatol. 2009; 22(2):50-59.
  11. Lee SK, Lee Y, Shin SH, Kim EY, Shin BC. Effects of Coptidis Rhizoma on the Anti-inflammation and Motor Recovery in Photothrombotic Brain Infarction Model in Rats. Kor J Herbol. 2009;24(1):179-89.
  12. Kim DI, Cheong HJ, Cheong SK, Lee HK. The Inhibitory Effects of Schizandrae Fructus and Coptidis Rhizoma on the IL-6, IL-16, GM-CSF mRNA level of BEAS-2B Human Epithelial Cells. 경희의학. 2001;17(2):199-213.
  13. Schinella GR, Tournier HA, Prieto JM, Mordujovich de Buschiazzo P, Ríos JL. Antioxidant activity of anti-inflammatory plant extracts. J Life Sci. 2002;70(9):1023-33. https://doi.org/10.1016/S0024-3205(01)01482-5
  14. Kim EH, Shim B, Kang S, Jeong G, Lee JS, Yu YB, Chun M. Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules. J Ethnopharmacol. 2009;126(2):320-31. https://doi.org/10.1016/j.jep.2009.08.027
  15. Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 2009; 125(2):286-90. https://doi.org/10.1016/j.jep.2009.06.027
  16. Jang SI. Wogonin inhibits Cytokine-induced TARC/CCL17 Expression by Suppression of NF-${\kappa}B$ activation via p38 MAP kinase Signalning Pathways in HaCaT Keratinocytes. Kor J Ori Med Physiol Pathol. 2007;21:1017-24.
  17. Mabalirajan U, Ahmad T, Rehman R, Leishangthem GD, Dinda AK, Agrawal A, Ghosh B, Sharma SK. Baicalein reduces airway injury in allergen and IL-13 induced airway inflammation. PLoS One. 2013;8(4):e62916. https://doi.org/10.1371/journal.pone.0062916
  18. Shieh DE, Chen YY, Yen MH, Chiang LC, Lin CC. Emodin-induced apoptosis through p53-dependent pathway in human hepatoma cells. J Life Sci. 2004;74:2279-90. https://doi.org/10.1016/j.lfs.2003.09.060
  19. Hei ZQ, Huang HQ, Tan HM, Liu PQ, Zhao LZ, Chen SR, Huang WG, Chen FY, Guo FF. Emodin inhibits dietary induced atherosclerosis by antioxidation and regulation of the sphingomyelin pathway in rabbits. Chin Med J (Engl). 2006;119:868-70.
  20. Kitano A, Saika S, Yamanaka O, Ikeda K, Okada Y, Shirai K, Reinach PS. Emodin suppression of ocular surface inflammatory reaction. Invest Ophthalmol Vis Sci. 2007;48:5013-22. https://doi.org/10.1167/iovs.07-0393
  21. Li HL, Chen HL, Li H, Zhang KL, Chen XY, Wang XW, Kong QY, Liu J. Regulatory effects of emodin on NF-kappaB activation and inflammatory cytokine expression in RAW 264.7 macrophages. Int J Mol Med. 2005;16: 41-7.
  22. Ha MK, Song YH, Jeong SJ, Lee HJ, Jung JH, Kim B, Song HS, Huh JE, Kim SH. Emodin inhibits proinflammatory responses and inactivates histone deacetylase 1 in hypoxic rheumatoid synoviocytes. Biol Pharm Bull. 2011;34(9):1432-7. https://doi.org/10.1248/bpb.34.1432
  23. Kang H, Kwon HA, So HJ, Lee JM, Lew JH, Choi HY. Effect of Samhwangsashim-tang Extract on Lipopolysaccharide-Stimulated Inflammatory Response and Macrophage Activity. Kor J Herbol 2. 012;27(6):23-8.
  24. Kim EJ, Kim HY, Lee JY, Lee JK, Kim SJ, Choi KM, Kang DA. Effect of Samhwangsasim-tang, Samigangaptang and Bangtan-tang on blood pressure in strok prone spontaneously hypertensive rats. Kor J Herbol. 2011; 26(1):75-80.
  25. Kim JB, Kwon OK, Son CW, Shin HM. Vasodilatory effects of Samhwangsasim-tang on vascular smooth muscle. Kor J Ori Med Physiol. Pathol 2004;8(5):1382-6.
  26. Lo YC, Tsai PL, Huang YB, Shen KP, Tsai YH, Wu YC, Lai YH, Chen IJ. San-Huang-Xie-Xin-Tang reduces lipopolysaccharide-induced hypotension and inflammatory mediators. J Ethnopharmacol. 2005;96(1-2):99-106. https://doi.org/10.1016/j.jep.2004.09.023
  27. Tsai HH, Chen IJ, Lo YC. Effects of San-Huang-Xie-Xin-Tang on U46619-induced increase in pulmonary arterial blood pressure. J Ethnopharmacol. 2008;117(3):457-62. https://doi.org/10.1016/j.jep.2008.02.024
  28. Wang YS, Lin RT, Cheng HY, Yang SF, Chou WW, Juo SH. Anti-therogenic effect of San-Huang-Xie-Xin-Tang, a traditional Chinese medicine, in cultured human aortic smooth muscle cells. J Ethnopharmacol. 2011;133(2): 442-7. https://doi.org/10.1016/j.jep.2010.10.018
  29. Park EJ, Kim B, Eo H, Park K, Kim Y, Lee HJ. Control of IgE and selective TH1 and TH2 cytokines by PG102 isolated from Actinidia arguta. J Allergy Clin Immunol. 2005;116:1151-7. https://doi.org/10.1016/j.jaci.2005.07.024
  30. 임현호. 알레르기성 비염의 통계적 관찰. 대한이비인후과학회지. 1984;27(3):247-53.
  31. 김정희. 아토피피부염의 최신 지견. 소아알레르기 및 호흡기 과학회지. 2004;14(1):12-23.
  32. 丁奎萬. 알레르기와 韓方. 서울:圖書出版第一路. 1993:59.
  33. Kay AB. Allergy and allergic disease. First of two parts. N Engl J Med. 2001;344:30-7. https://doi.org/10.1056/NEJM200101043440106
  34. Callerame ML, Conemi JJ, Ishizaka K. Immunoglobulin in bronchial tissues from patients with asthma, with special refernece to immunoglobulin E. J Allergy. 1971;47:187-95.
  35. Brown WR, Borthistle BK, Chen ST. Immunoglobulin E(IgE) and IgE-containing cells in human gastrointestinal fluidsand tissues. Clin Exp Immunol. 1975;20:227-37.
  36. Ahmed I, Nasreen S. Frequency of raised serum IgE level in childhood atopic dermatitis. J Pak Med Assoc. 2007;57:431-4.
  37. Hanifin JM. Rajka G. Diagnostic features of atopic dermatitis. Acta Derm Venerol. 1980;92:44-7.
  38. Leung, D.Y.M. Pathogenesis of atopic dermatitis. J Allergy Clin Immunol. 1999;104:99-108. https://doi.org/10.1016/S0091-6749(99)70150-8
  39. Mosmann TR, Coffman RI. Th1 and Th2 cells; Different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 1989;7:145-73. https://doi.org/10.1146/annurev.iy.07.040189.001045
  40. 홍정효. 아토피의 치료에 관한 양방과 한방, 민간요법과 자연치유법에 관한 고찰. 생태유아교육연구. 2008;7(1):65-85.
  41. Moingeon P, Batard T, Fadel R, Frati F, Sieber J , Van Overtvelt L. Immune mechanisms of allergen-specific sublingual immunotherapy. J Allergy. 2006;61:151-65. https://doi.org/10.1111/j.1398-9995.2006.01002.x
  42. Passalacqua G, Durham SR. Allergic rhinitis and its impact on asthma update: allergen immunotherapy. J Allergy Clin Immunol 2007;119:881-91. https://doi.org/10.1016/j.jaci.2007.01.045
  43. 손대범. 淸肌散과 三黃洗劑加味方 병용이 NC/Nga mice의 아토피 피부염에 미치는 영향. 원광대학교대학원. 2008.
  44. 정선영. 苦參(Sophorae Radix)이 생쥐의 B-cells에 미치는 항알레르기 효과. 경희대학교 대학원. 2002.
  45. 이상현, 윤용갑. 아토피 피부염 치료 처방에 대한 方劑學的 考察. 한국전통의학지. 2006;15(1):56-69.
  46. 康秉秀편. 漢方臨床알레르기. 서울:成輔社. 1988:23.
  47. 巢元方. 諸病源候論校釋. 北京:人民衛生出版社. 1983:18- 20, 170-2.
  48. 김미정, 이승연. 소아 아토피 피부염에 대한 문헌적 고찰. 대한한방소아과학회지. 2000;14:169-84.
  49. 박민철, 김진만, 홍철희, 황충연. 아토피 피부염의 東西醫學的文獻考察. 대한안이비인후과학회지. 2002;15(1):226-5.
  50. Lee IC, Kim BH, Kim MK. Investigations of Samwhang-sasimtang Extracts on Biological Activities In Vitro and Vivo. Lab Anim Res. 2010;26(1):15-20. https://doi.org/10.5625/lar.2010.26.1.15
  51. Won SH, Owi JS, Choi EJ, Kwon KR. A clinical case study of Acupuncture treatment for burned hand. The Acupuncture. 2005;22(1):13-7.
  52. Park YK, Jeong HW, Kim CM, Choi JS, Kim YS. Effects of Berberine on the production of inflammatory mediators from LPS-stimulated BV2 microglial cells. Kor J Herbol. 2007;22(4):117-25.
  53. Feng YB. Luo WQ, Zhu SQ. Explore new clinical application of Huang Lian and corresponding compound prescriptions from their traditional use. Zhong-guo Zhong Yao Za Zhi. 2008;10:1221-5.
  54. Kang H, Bang TS, Lee JW, Lew JH, Eom SH, Lee K, Choi HY. Protective effect of the methanol extract from Cryptotaeniae japonica Hassk against lipopolysaccharide-induced inflammation invitro and in vivo. BMC Complementary and Alternative Medicine. 2012;12:199. https://doi.org/10.1186/1472-6882-12-199
  55. Luo Y, Zhao H, Liu Z, Ju D, He X, Xiao C, Zhong G, Chen S, Yang D, Chan AS, Lu A. Comparison of the enteric mucosal immunomodulatory activity of combinations of Coptis chinensis Franch. Rhizomes and Evodia rutaecarpa (Juss.) Benth. Fruits in mice with dextran sulphate sodium-induced ulcerative colitis. Planta Med. 2010;76(8):766-72. https://doi.org/10.1055/s-0029-1240701
  56. Lee WC, Kim JK, Kang JW, Oh WY, Jung JY, Kim YS ,Jung HA, Choi JS, Lee SM. Palmatine attenuates D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure in mice. Food Chem Toxicol. 2010;48:222-8. https://doi.org/10.1016/j.fct.2009.10.004
  57. Lu JS, Liu YQ, Li M, Li BS, Xu Y. Protective effects and its mechanisms of total alkaloids from rhizoma Coptis chinensis on Helicobacter pylori LPS induced gastric lesion in rats. Zhongguo Zhong Yao Za Zhi. 2007;32(13): 1333-6.
  58. Zhang H, Tian K, Tang J, Qi S, Chen H, Chen X, Hu Z. Analysis of baicalein, baicalin and wogonin in Scutellariae radix and its preparation by microemulsion electrokinetic chromatography with 1-butyl-3-methylimizolium tetrafluoborate ionic liquid as additive. J Chromatogr A. 2006;1129(2):304-7. https://doi.org/10.1016/j.chroma.2006.08.016
  59. Arichi H, Kimura Y, Okuda H, Baba K, Kozawa M, Archi S. Effects of stilbene components of the roots of Polygonum cuspidatum Sieb. et Zucc. on lipid metabolism. Chemical Pharmaceutical Bulletin. 1982;30:1766-70. https://doi.org/10.1248/cpb.30.1766
  60. Leung DY, Bieber T. Atopic dermatitis. Lancet. 2003; 361(9352):151-60. https://doi.org/10.1016/S0140-6736(03)12193-9
  61. Mohrenschlager, M., Darsow, U., Schnopp, C., Ring, J. Atopic eczema: what's new? J Eur Acad Dermatol Venereol. 2006;20:503-13. https://doi.org/10.1111/j.1468-3083.2006.01580.x
  62. Barnes PJ. Pathophysiology of allergic inflammation. Immunological reviews. 2011;242(1):31-50. https://doi.org/10.1111/j.1600-065X.2011.01020.x
  63. Teixeira LK, Fonseca BP, Barboza BA, Viola JP. The role of interferon-${\gamma}$ on immune and allergic responses. Memorias do Instituto Oswaldo Cruz 100 Suppl. 2005;1: 137-44.
  64. Yamaoka KA, Dugas B, Paul-Eugene N, Mencia-Huerta JM, Braquet P, Kolb JP. Leukotriene B4 enhances IL-4-induced IgE production from normal human lymphocytes. Cell Immunol. 1994;156(1):124-34. https://doi.org/10.1006/cimm.1994.1158
  65. Lee IC, Kim BH, Kim SO, Kim MK. Skin Safety Evaluation of a Korean Traditional Prescription, Dansam-samul- tang and Samwhang-sasimtang. Kor J Ori Med Physiol Pathol. 2011;25(2):212-6.