Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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GST2 is Required for Nitrogen Starvation-Induced Filamentous Growth in Candida albicans

  • Lee, So-Hyoung (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Chung, Soon-Chun (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Shin, Jongheon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Oh, Ki-Bong (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2014.05.08
  • Accepted : 2014.06.12
  • Published : 2014.09.28
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Abstract

Candida albicans, the major human fungal pathogen, undergoes morphological transition from the budding yeast form to filamentous growth in response to nitrogen starvation. In this study, we identified a new function of GST2, whose expression was required for filamentous growth of C. albicans under nitrogen-limiting conditions. The Gst2p showed Gst activity and required response to oxidative stress. The ${\Delta}gst2$ mutant displayed predominantly yeast phase growth in low ammonium media. Such morphological defect of ${\Delta}gst2$ mutants was not rescued by overexpression of Mep2p, Cph1p, or Efg1p, but was rescued by either overexpression of a hyperactive $RAS1^{G13V}$ allele or through exogenous addition of cyclic AMP. In addition, the ${\Delta}gst2$ mutants had lower levels of RAS1 transcripts than wild-type cells under conditions of nitrogen starvation. These results were consistent with the Ras1-cAMP pathway as a possible downstream target of Gst2p. These findings suggest that Gst2p is a significant component of nitrogen starvation-induced filamentation in C. albicans.

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