Journal of Microbiology and Biotechnology

  • 제24권9호
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  • Pages.1170-1177
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  • 2014
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Toxic Pyrene Metabolism in Mycobacterium gilvum PYR-GCK Results in the Expression of Mammalian Cell Entry Genes as Revealed by Transcriptomics Study

  • Badejo, Abimbola Comfort (Department of Molecular and Life Science, Hanyang University) ;
  • Chung, Won Hyong (Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Nam Shin (Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Se Kye (Department of Molecular and Life Science, Hanyang University) ;
  • Chai, Jin Choul (Department of Molecular and Life Science, Hanyang University) ;
  • Lee, Young Seek (Department of Molecular and Life Science, Hanyang University) ;
  • Jung, Kyoung Hwa (Department of Molecular and Life Science, Hanyang University) ;
  • Kim, Hyo Joon (Department of Molecular and Life Science, Hanyang University) ;
  • Chai, Young Gyu (Department of Molecular and Life Science, Hanyang University)
  • 투고 : 2013.12.02
  • 심사 : 2014.06.09
  • 발행 : 2014.09.28
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초록

Mycobacterium gilvum PYR-GCK is a bacterial strain under study for its bioremediation use on heavy hydrocarbon pollutants in the environment. During the course of our study, mammalian cell entry (mce) genes, known to facilitate pathogenicity in M. tuberculosis, were highly expressed during a comparative and substrate-related cultural global transcriptomic study. RNA sequencing of the global transcriptome of the test strain in two different substrates, pyrene and glucose, showed high expression of the mce genes based on the differential results. After validating the expression of these genes with quantitative real-time PCR, we arrived at the conclusion that the genes were expressed based on the pyrene substrate (a phytosterol compound), and sterol metabolism is said to activate the expression of the mce genes in some actinomycetes bacteria, M. gilvum PYR-GCK in this case. This study is believed to be important based on the fact that some mycobacterial strains are undergoing a continuous research as a result of their use in practical bioremediation of anthropogenic exposure of toxic organic wastes in the environment.

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