Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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The analysis of useful components in Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium

팽이버섯 자실체, 팽이버섯 균사체 및 동충하초 균사체의 유용성분 분석

  • 김용두 (순천대학교 식품공학과) ;
  • 곽상호 (순천청암대학교 포장물류학과) ;
  • 김경제 ((재)장흥군버섯산업연구원) ;
  • 서경순 ((재)장흥군버섯산업연구원) ;
  • 박태영 ((재)장흥군버섯산업연구원) ;
  • 유강열 ((재)전주생물소재연구소) ;
  • 진성우 ((재)장흥군버섯산업연구원)
  • Received : 2014.07.11
  • Accepted : 2014.09.12
  • Published : 2014.09.30
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Abstract

Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium were analyzed for their proximate composition, protein-bound polysaccharide, nucleic acid and amino acids. The content of ash and crude fiber in F. velutipes fruit body were higher than F. mycelium and C. militaris mycelium. C. militaris mycelium showed the highest crude fat content while F. velutipes fruit body had lowest. Nitrogen free extract content of the samples varied from 56.8% in F. velutipes fruit body to 61.9% in F. velutipes mycelium. The compositions of total protein and total free sugars of protein-bound polysaccharide were found to be significant differences for all samples. Nucleic acid related compounds were identified the 5'-GMP, 5'-XMP, 5'-IMP in all samples. The content of total nucleic acids were high in the orders of F. velutipes myclial (286.71 mg%), F. velutipes fruit body(187.36 mg%) and C. militaris mycelial(76.85 mg%). The highest content of 5'-GMP was found in F. velutipes fruit body. The most nucleic acid of F. velutipes mycelial and C. militaris mycelial were the 5'-XMP. As for the analysis of total amino acids, seventeen amino acids were identified by HPLC and the major amino acid was glutamic acid in all samples. The content of total amino acids were high in the orders of F. velutipes fruit body(19,919 mg%), F. velutipes mycelium(19,018 mg%) abd C. militaris mycelium(18,965 mg%). We determined the developing new food product such as amino acid drink and amino acid containing food using extracts of Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium.

일반성분 중 조회분 함량은 버섯이 균사체 보다 높은 조회분 함량을 보였다. 조단백질은 모든 시험구에서 19% 내외로 나타났고, 조지방 함량은 동충하초 균사체에서 가장 높았으며, 조섬유는 팽이버섯에서 가장 높게 나타났다. 가용성물질소물은 팽이버섯균사체와 동충하초균사체에서 60% 이상의 함량을 나타내었다. 각 시료별 총단백질과 총당의 구성 비율은 팽이버섯과 팽이버섯 균사체의 단백다당류의 구성이 다름을 확인하였다. 시료별 핵산관련물질 함량은 팽이버섯에서 5'-GMP, 5'-XMP, 5'-IMP순으로 높게 나타났고, 팽이버섯 균사체는 5'-XMP, 5'-GMP, 5'-IMP, 동충하초 균사체는 5'-XMP, 5'-IMP, 5'-GMP순으로 높게 나타났다. 모든시료에서 각각 17종의 아미노산이 검출되었다. 각 시료의 총 아미노산 함량을 살펴보면 팽이버섯 18,980 mg%, 팽이버섯 균사체 17,604 mg%, 동충하초 균사체 18,429 mg%로 나타났고 모든 시료에서 glutamic acid 함량이 가장 높았다. 유리아미노산 함량도 구성아미노산과 유사하게 팽이버섯, 팽이버섯 균사체, 동충하초 균사체 순으로 높게 나타났다. 본 실험결과 팽이버섯, 팽이버섯 균사체 및 동충하초 균사체는 핵산관련 물질과 아미노산 함량이 높게 나타나, 아미노산 함유 식품 개발에 적합한 자원으로 활용도가 기대된다.

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References

  1. A.O.A.C. 1984. Official methodes Analysis 14th de. Associations of official analytical chemists. Washington DC. pp. 31-47.
  2. Chang St, Miles DG. 1989. The nutritional attributes and medicinal value of edible mushrooms. In edible mushrooms and their cultivation. Boca Raton. FL: CRC Press. pp. 27-40.
  3. Cho SH, Lee SD, Lee HU, Kim NG, Ryu JS, Lee DS. 1998. Effects of sawdust removal on root part enoki mushroom (Flammulina velutipes) on quality during storage. Korean J. Postharvest Sci. Technol. 5:231-238.
  4. Hong JS, Kim YH, Kim MK, Kim YS, Sohn HS. 1989. Contents of free amino acids and total amino acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Korean J. Food Sci. Technol. 21:58-62.
  5. Jeong DH. 1984. The latest food analysis method. Samjung Publishing Co. pp 119.
  6. Jo WS, Nam BH, Oh SJ, Choi YJ, Kang EY, Hong SH, Lee SH, Jeong MH. 2008. Hepatic protective effect and single-dose toxicity study of water extract of Cordyceps militaris grown upon Protaetia dreujtarsis. Korean. J. Food Sci. Technol. 40:1-5.
  7. Kang BH, Lee SH, Hur SS, Shin TK, Lee DS, Chang HB, Song BJ, Lee JM. 2010. The cultural characteristics of Paecilomyces tenuipes. Korean J. Food Preserv. 17:365-369.
  8. Kim HS, Son SR, Hwang SY, Hong BS. 1999. Purification and characterization of the lectins from mushroom Flammulina velutipes. J. Korean Soc. Agric. Chem. Biotechnol. 42:304-309.
  9. Kim MS, Kim GH. 2010. Contents of nucleic acids(Nucleosides and mono-nucleotides) in extracts of Pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes. Korean J. Food & Nutr. 23:376-380.
  10. Kim HS, Kim MA, Jang SH, Lee WK, Ryu JY, Lee CS. 2012. Anti-hyperlipidemic effects of Cordyceps militaris hot-water extract. J. Environ. Sci. 21:875-881. https://doi.org/10.5322/JES.2012.21.7.875
  11. Kim HS, Kim MA, Jang SH, Kang DS, Lee WK, Ryu JY, Lee CS. 2013. Effects of Cordyceps militaris on the improvement actions of blood glucose and atherosclerosis in diabetes. J. Environ. Sci. 22:341-346. https://doi.org/10.5322/JESI.2013.22.3.341
  12. Kim OK. 2011. Antidiabetic metabolism effect on the water extract of Cordyceps militaris in streptozotocin-induced diabetic rats. Korean J. Oil Chemists' Soc. 28:267-272.
  13. Kim CS. 2010. Rheological properties of bread dough made from Cordyceps militaris powder. Korean J Food & Nutr. 23:8-14.
  14. Kim MS, Kim GH. 2010. Contents of nucleic acids (Nucleosides and mono-nucleotides) in extracts of pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes. Korean J. Food & Nutr. 23:376-380.
  15. Ko SK, Kim JS, Choi YE, Lee SJ, Park KS, Chung SH. 2002. Anti-diabetic effect of mixed water extract from ginseng radix rubra, Acanthopanacis cortex and Cordyceps. Korean J. Pharmacogn. 33:337-342.
  16. Koh JB. 2002. Effect of mycelium of Cordyceps militaris on growth, lipid metabolism and protein levels in male rats. J. Korean Soc. Food Sci. Nutr. 31:685-690. https://doi.org/10.3746/jkfn.2002.31.4.685
  17. Lee SA, Song YS, Cho JW, Lee JH, Cho JS. 2001. Effect of the Sarcodon aspratus on the physicochemical and sensory properties of cooked beef. J. Korean Soc. Food Sci. Nutr. 30:266-272.
  18. Li Y, Ishikaw Y, Satake T, Kitazawa H, Qiu X, Rungchang S. 2014. Effect of active modified atmosphere packaging with different initial gas compositions on nutritional compounds of shiitake mushrooms (Lentinus edodes). Postharvest Biol. Technol. 92:107-113. https://doi.org/10.1016/j.postharvbio.2013.12.017
  19. Nam SH, Lee KG, Yeo JH, Lee HS, Hwang JS, Choi YC, Park KH. 2012. Cultivation of Paecilomyces tenuipes using mini-kit, small culture container. J. Seric. Entomol. Sci. 50:116-121. https://doi.org/10.7852/jses.2012.50.2.116
  20. Oh SI, Lee MS. 2010. Functional activities of ethanol extracts from Flammulina velutipes. Korean J. Food & Nutr. 23:15-22.
  21. Ohara I, Ariyoshi S. 1979. Comparison of protein precipitants for the determination of free amino acid in plasma. Agric. Biol. Chem. 43:1473. https://doi.org/10.1271/bbb1961.43.1473
  22. Park KM, Lee BW. 1998. Extraction and purification of antitumor protein-bound polysaccharides from mycelia of Lentinus edodes. Korean J. Food Sci. Technol. 30:1236-1242.
  23. Park YH. 1997. The latest mushroom science. Korean Mushroom Seed Combination. Seoul. pp 395-458.
  24. Pyo HJ, Son DY, Lee C. 2002. Purification and characterization of polyphenol oxidase from Flammulina velutipes. Korean J. Food Sci. Technol. 34:552-558.
  25. Shin SH, Jung SJ, Choi JH, Kim DM, Jeong MC. 2009. Effect of packaging methods on enoki mushroom qualities. Korean J. Food Preserv. 15:179-185.
  26. Shin SM, Kim AJ, Cho HC, Joung KH. 2008. Quality characteristics of Seolgiddeok prepared with added Paecilomyces japonica powder. Korean J. Food & Nutr. 21:22-27.
  27. Stamets P. 2000. Growing gourmet and medicinal mushrooms. Ten Speed Press. Berkely. California. pp 574.
  28. Yamaguchi S, Yosikawa T, Ikeda S, Ninomiya T. 1971. Measurement of the relative taste intensity of some $\alpha$-amino acid and 5'-nucleotides. J. Food Sci. 36:846-849. https://doi.org/10.1111/j.1365-2621.1971.tb15541.x
  29. Yang JH, Lin HC, Maub JL. 2001. Non-volatile taste components of several commercial mushrooms. Food Chem. 72:465-471. https://doi.org/10.1016/S0308-8146(00)00262-4