한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제22권5호
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  • Pages.339-348
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  • 2014
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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차세대염기서열 분석을 이용한 고려인삼과 미국삼의 전사체 분석

Characterization of Root Transcriptome among Korean Ginseng Cultivars and American Ginseng using Next Generation Sequencing

  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김선태 (부산대학교 식물생명과학과) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김기홍 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김홍식 (충북대학교 식물자원학과) ;
  • 정종욱 (농촌진흥청 국립유전자원센터) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Jo, Ick Hyun (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Chang (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Seung Ho (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jang Uk (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Sun Tae (Department of Plant Bioscience, Pusan National University) ;
  • Hyun, Dong Yun (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Dong Hwi (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Kee Hong (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Hong Sig (Department of Plant Resources, Chungbuk National University) ;
  • Chung, Jong Wook (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Bang, Kyong Hwan (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA)
  • 투고 : 2014.06.25
  • 심사 : 2014.08.06
  • 발행 : 2014.10.30
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초록

The transcriptomes of four ginseng accessions such as Cheonryang (Korean ginseng cultivar), Yunpoong (Korean ginseng cultivar), G03080 (breeding line of Korean ginseng), and P. quinquefolius (American ginseng) was characterized. As a result of sequencing, total lengths of the reads in each sample were 156.42 Mb (Cheonryang cultivar), 161.95 Mb (Yunpoong cultivar), 165.07 Mb (G03080 breeding line), and 166.48 Mb (P. quinquefolius). Using a BLAST search against the Phytozome databases with an arbitrary expectation value of 1E-10, over 20,000 unigenes were functionally annotated and classified using DAVID software, and were found in response to external stress in the G03080 breeding line, as well as in the Cheonryang cultivar, which was associated with the ion binding term. Finally, unigenes related to transmembrane transporter activity were observed in Cheonryang and P. quinquefolius, which involves controlling osmotic pressure and turgor pressure within the cell. The expression patterns were analyzed to identify dehydrin family genes that were abundantly detected in the Cheonryang cultivar and the G03080 breeding line. In addition, the Yunpoong cultivar and P. quinquefolius accession had higher expression of heat shock proteins expressed in Ricinus communis. These results will be a valuable resource for understanding the structure and function of the ginseng transcriptomes.

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