Mycobiology

The Korean Society of Mycology (한국균학회)
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Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus

  • Jang, In-Taek (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Shin, Ja-Won (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Yun-Hae (Mushroom Research Station, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Ji, Jeong-Hyun (Mushroom Research Station, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • Received : 2014.07.12
  • Accepted : 2014.07.31
  • Published : 2014.09.30
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Abstract

We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus ostreatus fruiting body was treated with distilled water at $40^{\circ}C$ for 48 hr. The XOD inhibitor thus obtained was purified by Sephadex G-50 gel permeation chromatography, ultrafiltration, $C_{18}$ solid phase extraction chromatography and reverse-phase high-performance liquid chromatography with 3% of solid yield, and its XOD inhibitory activity was 0.9 mg/mL of $IC_{50}$. The purified XOD inhibitor was a tripeptide with the amino acid sequence phenylalanine-cysteine-histidine and a molecular weight of 441.3 Da. The XOD inhibitor-containing ultrafiltrates from Pleurotus ostreatus demonstrated dose-dependent anti-gout effects in a Sprague-Dawley rat model of potassium oxonate-induced gout, as shown by decreased serum urated levels at doses of 500 and 1,000 mg/kg, although the effect was not as great as that achieved with the commercial anti-gout agent, allopurinol when administered at a dose of 50 mg/kg.

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References

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