Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluating Carriers for Immobilizing Saccharomyces cerevisiae for Ethanol Production in a Continuous Column Reactor

  • Cha, Hye-Geun (Department of Biotechnology, Korea National University of Transportation) ;
  • Kim, Yi-Ok (Department of Biotechnology, Korea National University of Transportation) ;
  • Choi, Woon Yong (Department of Medical Biomaterials Engineering, Kangwon National University) ;
  • Kang, Do-Hyung (Korea Institute of Ocean Science and Technology) ;
  • Lee, Hyeon-Yong (Department of Food Science and Engineering, Seowon University) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)
  • Received : 2014.02.13
  • Accepted : 2014.05.05
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

We evaluated a more practical and cost-effective immobilization carriers for ethanol production using the yeast Saccharomyces cerevisiae. Three candidate materials-rice hull, rice straw, and sawdust-were tested for their cell-adsorption capacity and operational durability. Derivatizations of rice hull, rice straw, and sawdust with the optimal concentration of 0.5 M of 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl) resulted in > 95% adsorption of the initial yeast cells at 2 hr for DEAE-rice hull and DEAE-sawdust and in only approximately 80% adsorption for DEAE-rice straw. In addition, DEAE-sawdust was found to be a more practical carrier for immobilizing yeast cells in terms of operational durability in shaking flask cultures with two different speeds of 60 and 150 rpm. Furthermore, the biosorption isotherms of DEAE-rice hull, -rice straw, and -sawdust for yeast cells revealed that the $Q_{max}$ of DEAE-sawdust (82.6 mg/g) was greater than that of DEAE-rice hull and DEAE-rice straw. During the 404-hr of continuous column reactor operation using yeast cells immobilized on DEAE-sawdust, no serious detachment of the yeast cells from the DEAE-sawdust was recorded. Ethanol yield of approximately 3.04 g/L was produced steadily, and glucose was completely converted to ethanol at a yield of 0.375 g-ethanol/g-glucose (73.4% of the theoretical value). Thus, sawdust is a promising practical immobilization carrier for ethanol production, with significance in the production of bioethanol as a biofuel.

Keywords

References

  1. Kourkoutas Y, Bekatorou A, Banat IM, Marchant R, Koutinas AA. Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiol 2004;21:377-97. https://doi.org/10.1016/j.fm.2003.10.005
  2. Mussatto SI, Dragone G, Guimaraes PM, Silva JP, Carneiro LM, Roberto IC, Vicente A, Domingues L, Teixeira JA. Technological trends, global market, and challenges of bioethanol production. Biotechnol Adv 2010;28:817-30. https://doi.org/10.1016/j.biotechadv.2010.07.001
  3. Singh A, Sharma P, Saran AK, Singh N, Bishnoi NR. Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices. Renew Energy 2013;50:488-93. https://doi.org/10.1016/j.renene.2012.07.003
  4. Mathew AK, Crook M, Chaney K, Humphries AC. Comparison of entrapment and biofilm mode of immobilisation for bioethanol production from oilseed rape straw using Saccharomyces cerevisiae cells. Biomass Bioenergy 2013;52:1-7. https://doi.org/10.1016/j.biombioe.2013.02.028
  5. Bouallagui H, Touhami Y, Hanafi N, Ghariani A, Hamdi M. Performances comparison between three technologies for continuous ethanol production from molasses. Biomass Bioenergy 2013;48:25-32. https://doi.org/10.1016/j.biombioe.2012.10.018
  6. Yu J, Zhang X, Tan T. An novel immobilization method of Saccharomyces cerevisiae to sorghum bagasse for ethanol production. J Biotechnol 2007;129:415-20. https://doi.org/10.1016/j.jbiotec.2007.01.039
  7. Liang L, Zhang YP, Zhang L, Zhu MJ, Liang SZ, Huang YN. Study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses. J Ind Microbiol Biotechnol 2008;35:1605-13. https://doi.org/10.1007/s10295-008-0404-z
  8. Plessas S, Bekatorou A, Koutinas AA, Soupioni M, Banat IM, Marchant R. Use of Saccharomyces cerevisiae cells immobilized on orange peel as biocatalyst for alcoholic fermentation. Bioresour Technol 2007;98:860-5. https://doi.org/10.1016/j.biortech.2006.03.014
  9. Pacheco AM, Gondim DR, Goncalves LR. Ethanol production by fermentation using immobilized cells of Saccharomyces cerevisiae in cashew apple bagasse. Appl Biochem Biotechnol 2010;161:209-17. https://doi.org/10.1007/s12010-009-8781-y
  10. Branyik T, Silva DP, Vicente AA, Lehnert R, e Silva JB, Dostalek P, Teixeira JA. Continuous immobilized yeast reactor system for complete beer fermentation using spent grains and corncobs as carrier materials. J Ind Microbiol Biotechnol 2006;33:1010-8. https://doi.org/10.1007/s10295-006-0151-y
  11. Genisheva Z, Mussatto SI, Oliveira JM, Teixeira JA. Evaluating the potential of wine-making residues and corn cobs as support materials for cell immobilization for ethanol production. Ind Crops Prod 2011;34:979-85. https://doi.org/10.1016/j.indcrop.2011.03.006
  12. Bardi EP, Koutinas AA. Immobilization of yeast on delignified cellulosic material for room temperature and lowtemperature wine making. J Agric Food Chem 1994;42:221-6. https://doi.org/10.1021/jf00037a040
  13. Lee SE, Lee CG, Kang DH, Lee HY, Jung KH. Preparation of corncob grits as a carrier for immobilizing yeast cells for ethanol production. J Microbiol Biotechnol 2012;22:1673-80. https://doi.org/10.4014/jmb.1202.02049
  14. Lee SE, Kim YO, Choi WY, Kang DH, Lee HY, Jung KH. Two-step process using the immobilized Saccharomyces cerevisiae and Pichia stipitis for ethanol production from the hydrolysate of Ulva pertusa Kjellman. J Microbiol Biotechnol 2013;23: 1434-44. https://doi.org/10.4014/jmb.1304.04014
  15. Sarkar N, Ghosh SK, Bannerjee S, Aikat K. Bioethanol production from agricultural wastes: an overview. Renew Energy 2012;37:19-27. https://doi.org/10.1016/j.renene.2011.06.045
  16. Sobhanardakani S, Parvizimosaed H, Olyaie E. Heavy metals removal from wastewaters using organic solid waste-rice husk. Environ Sci Pollut Res Int 2013;20:5265-71. https://doi.org/10.1007/s11356-013-1516-1
  17. Song ST, Saman N, Johari K, Mat H. Removal of Hg(II) from aqueous solution by adsorption using raw and chemically modified rice straw as novel adsorbents. Ind Eng Chem Res 2013;52;13092-101. https://doi.org/10.1021/ie400605a
  18. Baki MH, Shemirani F, Khani R. Potential of sawdust as a green and economical sorbent for simultaneous preconcentration of trace amounts of cadmium, cobalt, and lead from water, biological, food, and herbal samples. J Food Sci 2013;78:T797-804. https://doi.org/10.1111/1750-3841.12104
  19. Vijayaraghavan K, Yun YS. Bacterial biosorbents and biosorption. Biotechnol Adv 2008;26:266-91. https://doi.org/10.1016/j.biotechadv.2008.02.002
  20. Chaplin MF, Kennedy JF. Carbohydrate analysis: a practical approach. Oxford: IRL Press; 1986.
  21. Lee SE, Lee JE, Shin GY, Choi WY, Kang DH, Lee HY, Jung KH. Development of practical and cost-effective medium for the bioethanol production from the seaweed hydrolysate in surface-aerated fermentor by repeated-batch operation. J Microbiol Biotechnol 2012;22:107-13. https://doi.org/10.4014/jmb.1106.06019
  22. Yeon JH, Lee SE, Choi WY, Kang DH, Lee HY, Jung KH. Repeated-batch operation of surface-aerated fermentor for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum. J Microbiol Biotechnol 2011;21:323-31.
  23. Vieira AM, Sa-Correia I, Novais JM, Cabral JM. Could the improvements in the alcoholic fermentation of high glucose concentrations by yeast immobilization be explained by media supplementation? Biotechnol Lett 1989;11:137-40. https://doi.org/10.1007/BF01192190
  24. Khare SK, Nakajima M. Immobilization of Rhizopus japonicus lipase on celite and its application for enrichment of docosahexaenoic acid in soybean oil. Food Chem 2000; 68:153-7. https://doi.org/10.1016/S0308-8146(99)00166-1