Reproductive and Developmental Biology

  • 제38권3호
  • /
  • Pages.93-98
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  • 2014
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
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Washing Culture Oil이 돼지 수정란의 체외 발육에 미치는 영향

Effect of Washing Culture Oil on In Vitro Development in Porcine Embryos

  • 이연주 (강원대학교 동물생명과학대학) ;
  • 김유진 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Lee, Yeon-Ju (College of Animal Life Science, Kangwon National University) ;
  • Kim, Yu-Jin (College of Animal Life Science, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University)
  • 투고 : 2014.08.21
  • 심사 : 2014.09.15
  • 발행 : 2014.09.30
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초록

The objective of this study was to investigate the changes of oxidative stress and antioxidant enzyme during in vitro development with washing culture oil in porcine embryos. During the culture, the four types of culture oil such as paraffin oil with or without washing and mineral oil with or without washing were examined. The oil was washed with PZM-3 during 7 days and collected oil only. The embryos were stained with CellTracker$^{TM}$ Red, DCFDA and Hoechst 33342 to confirm the effects of the oil. As a results, Cleavage rates and total cell number were no difference among the four oil groups. However, ${\geq}16$ cell embryos were significantly different in fore type oil treatment and blastocyst rate was significantly higher washing paraffin treatment than in other group(p<0.05). Also, the expression of free radical were lower in washing paraffin oil than in other groups (p<0.05). On the other hand, the expression of glutathione were not significant different among paraffin oil with or without washing and mineral oil with or without washing, however washing paraffin oil and washing mineral groups were higher than other treatment groups. In conclusion, the washing oil was expected with positive effects on in vitro development in porcine embryos.

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참고문헌

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