Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Structure of Egg Envelope and Oogenesis of the Korean Endangered Fish Gobiobotia brevibarba (Pisces: Cyprinidae)

멸종위기 어류 돌상어 Gobiobotia brevibarba의 난자형성과정과 난막의 형태

  • Choi, Wung Sun (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Kim, Jae Goo (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Park, Jong Young (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University)
  • 최웅선 (전북대학교 자연과학대학 생명과학과, 전북대학교 생물다양성연구소) ;
  • 김재구 (전북대학교 자연과학대학 생명과학과, 전북대학교 생물다양성연구소) ;
  • 박종영 (전북대학교 자연과학대학 생명과학과, 전북대학교 생물다양성연구소)
  • Received : 2014.09.01
  • Accepted : 2014.09.25
  • Published : 2014.09.30
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Abstract

A histological study on the egg envelope and oogenesis of Gobiobotia brevibarba (Pisces, Cyprinidae) was carried out by a light microscope and a scanning electron microscope. Various developmental cells appeared in the ovary caught during May 2014, spawning season. For the relative area of oocyte, the ovary consisted of mature stage (74.5%), a vitellogenic stage (yolk granule stage, 16.6% and yolk vesicle stage, 6.6%) and previtellogenic stage (perinucleolus stage 2.2%), which means its spawning season. The cytoplasm of the perinucleolus oocyte is acidic and many nucleoli are located at the inner side of the nuclear membrane. The yolk vesicles, an early vitellogenic stage, has a follicular layer and a zona radiata clearly. Numerous villi, called an egg envelope, begin to form on the zona radiata. The yolk granules, an another vitellogenic stage, proceeds and they show a strong eosinophilic nature. Such yolk granules appeared between the yolk vesicles occupying most cytoplasm, and as the stage proceeds, there are some yolk masses fused with each other. Egg envelope is covered with plenty of villi ($2{\sim}3{\mu}m$ in the length) over the entire egg surface.

잉어과(Pisces, Cyprinidae)에 속하는 멸종위기 어류인 돌상어 Gobiobotia brevibarba의 난막미세구조 및 난자형성과정을 광학현미경과 주사전자현미경을 이용하여 관찰하였다. 2014년 5월의 돌상어 난소 내에는 다양한 발생단계의 생식세포들이 관찰되었다. 난모세포들의 상대면적은 성숙란(74.5%), 난황물질이 형성되는 난황구기(16.6%), 난황포기(6.6%), 그리고 미숙한 발달단계인 주변인기(2.3%) 순으로 구성되어 있었다. 주변인기 단계의 세포질은 hematoxylin에 강하게 염색되며, 핵막의 안쪽에는 다수의 인들이 존재하였다. 난황형성물질인 난황포기와 초기 난황구기 단계에서는 여포층과 방사대가 뚜렷하게 형성되기 시작되었으며, 방사대 위에는 미세융모의 난막구조가 더욱 뚜렷해졌다. 난황구기 및 성숙란의 단계에서는 난황구 일부가 융합되면서 강한 호산성을 보였다. 또한 성숙란의 표면에는 깔때기 모양의 난문이 관찰되었으며, $2{\sim}3{\mu}m$ 길이의 미세융모가 일정하게 분포하였다.

Keywords

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