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양파(Allium cepa L.) 유전자원의 Flavonol 배당체 조성 및 함량

The Compositions and Contents of Flavonol Glycosides in Onion (Allium cepa L.) Germplasm

  • 이정로 (농촌진흥청 국립농업과학원) ;
  • 이민기 (농촌진흥청 국립농업과학원) ;
  • 김헌웅 (농촌진흥청 국립농업과학원) ;
  • 이성현 (농촌진흥청 국립농업과학원) ;
  • 이영민 (농촌진흥청 국립농업과학원) ;
  • 장환희 (농촌진흥청 국립농업과학원) ;
  • 황경아 (농촌진흥청 국립농업과학원) ;
  • 곽재균 (농촌진흥청 국립농업과학원) ;
  • 고호철 (농촌진흥청 국립농업과학원) ;
  • 김정봉 (농촌진흥청 국립농업과학원) ;
  • 조진웅 (충남대학교 농업생명과학대학)
  • 투고 : 2014.06.04
  • 심사 : 2014.07.21
  • 발행 : 2014.09.30

초록

국내외 75점의 흰색 및 자색 양파 유전자원으로부터 총 5종의 flavonol 배당체(Q34'diG, Q3G, Q4'G, I4'G, Q)를 분리 동정하였다. 양파 내 존재하는 flavonol 배당체는 aglycone인 quercetin 및 isorhamnetin에 하나 또는 두 개의 glucose가 결합된 형태로 검출되었다. 총 flavonol 함량 범위는 흰색 양파가 0.18-6.47 mg/g, 자색 양파가 2.39-6.47 mg/g 이었으며, 자색 양파가 평균 4.41 mg/g 로 흰색 양파(3.23 mg/g)보다 약 1.4배 높았다. 양파의 주요 성분으로는 Q34'diG 및 Q4'G 총함량 비율이 흰색 및 자색 양파에서 모두 약 85% 이상을 보였다.

Flavonols as a major kind of plant secondary metabolites are known for health-promoting compounds in onions (Allium cepa L.). The objectives of this study are to determine profiles of flavonol glycosides in different 75 onion accessions. A total of five flavonols (quercetin 3,4'-diglucoside, Q34'diG; quercetin 3-glucoside, Q3G; quercetin 4'-glucoside, Q4'G; isorhamnetin 4'-glucoside, I4'G; quercetin, Q) were identified from onion accessions. In positive ion mode using LC-ESI-MS, individual flavonols were confirmed from one and two glycosylation binding with aglycone such as quercetin and isorhamnetin. Total flavonol contents were distributed in white onion (range of 0.18-6.47 mg/g DW) and purple onion accessions (range of 2.39-6.47 mg/g), respectively. The mean of flavonol contents in purple onion (4.41 mg/g) showed 1.4-fold higher than white onion (3.23 mg/g). The Q34'diG and Q4'G were considered as the major compounds of flavonol glycosides in onion accessions.

키워드

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