Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Comparison of Germination Characteristics and Various Pre-treatment Methods for Enhancing Germination on Zoysiagrass

한국잔디 종자의 발아특성과 발아율 향상을 위한 다양한 전처리 방법의 비교

  • Han, Jeong-Ji (Southern Forest Resources Research Center, Korea Forest Research Institute) ;
  • Lee, Kwang-Soo (Southern Forest Resources Research Center, Korea Forest Research Institute) ;
  • Park, Yong-Bae (Southern Forest Resources Research Center, Korea Forest Research Institute) ;
  • Yang, Geun-Mo (Department of Green Landscape Architecture Science, Dankook Univ.) ;
  • Bae, Eun-Ji (Southern Forest Resources Research Center, Korea Forest Research Institute)
  • 한정지 (국립산림과학원 남부산림자원연구소) ;
  • 이광수 (국립산림과학원 남부산림자원연구소) ;
  • 박용배 (국립산림과학원 남부산림자원연구소) ;
  • 양근모 (단국대학교 녹지조경학과) ;
  • 배은지 (국립산림과학원 남부산림자원연구소)
  • Received : 2014.08.28
  • Accepted : 2014.09.17
  • Published : 2014.09.30
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Abstract

Zoysiagrass seeds had low germination or lack of germination uniformity under natural condition. To improve the final germination percentage of zoysiagrass seeds, we investigated the methods of proper pre-treatment to enhance germination rate of zoysiagrass. Seeds were subjected to the testa scarification and four types of seed priming methods, namely, hydro priming (distilled water), osmotic priming (polyethylene glycol -0.5 MPa, -1.0MPa, -2.0 MPa), halo priming (Potassium nitrate 100 mM, 200 mM, 400 mM), and solid matrix priming (seed : microcel-E : distilled water = 4:1:8) with and without 25% KOH treatment in the testa. The scarification improved the germination rate and reduced the germination time. Osmotic priming (PEG -0.5 MPa) with scarification were significantly increased the seed germination rate and decreased MGT, and $T_{50}$. Seed germination rate obtained by osmotic priming (PEG -0.5MPa) with scarification increased above 60% in comparison with natural condition. Conclusively, scarified and primed zoysiagrass seed germinated more rapidly and uniformly in the study. The present results suggested that PEG priming treatment with KOH treatment prior to sowing could improve germination percentage, and this method makes us establish the early zoysiagrasses in the field.

들잔디 종자의 발아를 향상시키기 위해서, 적정 종자 전처리 방법을 조사하였다. 종자들은 종피 연화 및 4가지 프라이밍 처리, 즉, 종피에 KOH 처리 유무에 따른 Hydro 프라이밍(증류수), Osmotic 프라이밍(폴리에틸렌 글리콜 -0.5MPa, -1.0MPa, -2.0MPa), Halo 프라이밍(질산칼륨 100 mM, 200 mM, 400 mM)과 Solid matrix 프라이밍(종자:microcel-E:증류수 = 4:1:8) 방법을 조합하였다. 종피 연화는 발아율을 향상시키고 발아소요시간을 단축시켰다. 종피 연화 처리 후 Osmotic 프라이밍(PEG -0.5 MPa)에서 발아율이 유의적으로 증가하였고, MGT, $T_{50}$이 감소하였다. 종자 발아율은 자연상태와 비교하여 60% 이상 증가시켰다. 결론적으로, 종피연화와 프라이밍 처리한 잔디종자는 더 빠르고 균일하게 발아를 하였다. 현 연구를 통해 KOH처리와 PEG 프라이밍 처리는 파종 시 발아율을 향상 시켜 초기 잔디 조성을 빠르게 할 수 있을 것으로 기대된다.

Keywords

References

  1. Afzal, I., Rauf, S., Basra, S.M.A. and Murtaza, G. 2008. Halopriming improves vigor, metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant Soil Environ. 54:382-388.
  2. Bae, E.J., Park, N.C., Lee, K.S., Lee, S.M., Choi, J.S., et al. 2010. Distribution and morphology characteristics of native zoysiagrasses (Zoysia spp.) growing in South Korea. Kor. Turfgrass Sci. 24:97-105. (In Korean)
  3. Bae, K.H., Ko, M.S., Kim, N.Y., Song, J.M., Kim. Y.J., et al. 2014. Effects of priming and ultra sound on seed germination from Ardisia crenata Sims, an ornamental plant. Kor. J. Nature Conservation 8:1-7. (In Korean) https://doi.org/10.11624/KJNC.2014.8.1.001
  4. Cetinbas, M. and Koyuncu, F. 2006. Improving germination of Prunus avium L. seeds by gibberellic acid, potassium nitrate and thiourea. Hort. Sci. 33:119-123.
  5. Chang, Y.D. and Lee, C.H. 2007. Effect of storage duration, temperature and priming treatment on seed germination of polygonatum odoratum var. pluriflorum. Kor. J. Plant Resources. 20:481-489. (In Korean)
  6. Cho, Y.S., Ahn, S.H., Yang, G.M. and Choi, J.S. 2007. Establishment speed of 'Zenith' zoysiagrass by plugging. Kor. Turfgrass Sci. 21:9-22. (In Korean)
  7. Choi, C.H. 2012. Effect of temperature and various pre-treatments on germination of Hippophae rhamnoides seeds. Kor. J. Plant Res. 25:132-141. (In Korean) https://doi.org/10.7732/kjpr.2012.25.1.132
  8. Coolbear, P., Francis, A. and Grierson, D. 1984. The effect of low temperature presowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J. Exp. Bot. 35:1609-1617. https://doi.org/10.1093/jxb/35.11.1609
  9. Copeland, L.O. and Mcdonald, M.B. 1985. Principles of seed science and technology. Burgess Publishing Co., Minneapolis, USA. pp. 103-120.
  10. Cutler, D.F., Alvin, K.L. and Price, C.E. 1982. The plant cuticle. pp. 1-32. Academic press. London, UK.
  11. Ellis, R.A. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9:373-409.
  12. Emmons, R.D. 1995. Turfgrass science and management. Delmer Publishers. NY, USA. pp. 50-51.
  13. Farooq, M., Barsa, S.M. and Wahid, A. 2006. Priming of field-sown rice seed enhances germination, seedling establishment, allometry and yield. Plant growth regulation. 49:285-294. https://doi.org/10.1007/s10725-006-9138-y
  14. Ghassemi, G.K. and Esmaeilpour, B. 2008. The effect of salt priming on the performance of differentially matured cucumber (Cucumus sativus) seeds. Not. Bot. Horti Agrobot. Cluj-Napoca. 36:67-70.
  15. Hartmann, H.T. and Kester, D.E. 1983. Plant propagation principles and practices. 4th ed. Prentice Hall Inc., Englewood Cliffs, New Jersey, USA.
  16. Harris, D. 1992. The effects of manure, genotype, seed priming, depth and date of sowing on the emergence and early growth of Sorghum bicolor (L.) moench in semi-arid Botswana. Soil Till. Res. 40:73-88.
  17. Heydecker, W., Higgins, J. and Gulliver, R.L. 1973. Accelated germination by osmotic seed treatment. Nature. 246:42-44. https://doi.org/10.1038/246042a0
  18. Jang, Y.S., Heo, S.I., Choi, S.R. and Wang, M.H. 2005. Effect of various pretreatment on germination in Zoysia japonica. Kor. Turfgrass Sci. 19:125-130. (In Korean)
  19. Jang, D.H., Park, N.I., Yang, S.W. and Sim, G.Y. 2011. Present situation of zoysiagrass (Zoysia spp.) culture, sod production, and bland by prefecture in Japan. Asian J. Turfgrass Sci. 25:229-236.
  20. Jeon, B.S., Kang, J.H., Cho, S.C., Kwon, H.S. Ryu, Y.S., et al. 2001. Presown seed treatments to promote seed germination of zoysiagrass (Zoysia japonica). Kor. J. Plant Res. 14:175-181. (In Korean)
  21. Kang, J.S. 2004. Effect of osmotic and solid matrix priming to improve germination and early growth of carrot and onion seed. J. Bio Environ. Cont. 13:135-142. (In Korean)
  22. Kang, J.S., Son. B.G., Cho, Y.W., Lee, Y.J., Kim, Y.C., et al. 2008. Effect of seed priming on the enhancement of seed germination in cool season turfgrass. J. Life Sci. 18:1096-1105. (In Korean) https://doi.org/10.5352/JLS.2008.18.8.1096
  23. Kang, K.S., Ahn, C.K., Lee, J.W., Choi, Y.W., Son, B.G., et al. 2005. Effects of seed coat scarification on improved germination in Korean lawngrass. Kor. J. Hort. Sci. Technol. 21:75 (Abstr.). (In Korean)
  24. Kang, N.J., Ok, J.Y., Lai, C.J. and Kang, S.M. 1997. Changes of seed proteins related to low temperature germinability of primed seeds of pepper (Capsicum annuum L.). Kor. J. Hort. Sci. Technol. 38:342-346. (In Korean)
  25. Khan, A.A. 1992. Preplant physiological seed conditioning. Hort. Rev. 13:131-181.
  26. Kim, K.N. 2009. Comparison of germination characteristics and daily seed germinating pattern in varieties of coarse-textured tall fescue under alternative and natural room temperature conditions. Kor. J. Turfgrass Sci. 23:23-34. (In Korean)
  27. Kim, K.N. and Jung, K.W. 2008. Comparison of seed germination vigor, early germination characteristics, germination speed and germination peak time in new varieties of the third gerrnination of creeping bentgrass under different growing conditions. J. Kor. Env. Res. Reveg. Tech. 11:79-91. (In Korean)
  28. Kim, K.N. and Park, S.H. 2010. Comparison of germination characteristics and daily seed germinating pattern in finetextured fescues. Kor. J. Hort. Technol. 28:567-573. (In Korean)
  29. Kim, T.J. 1995. Irreversible pregermination stages for primed zoysiagrass (Zoysia japonica Steud.) seed production. J. Kor. Soc. Hort. Sci. 36:391-400. (In Korean)
  30. Li, J., Yin, L.Y., Jongsma, M.A. and Wang, C.Y. 2011. Effects of light, hydropriming and abiotic stress on seed germination, and shoot and root growth of pyrethrum (Tanacetum cinerariifolium). Industrial crops and products. 34:1543-1549. https://doi.org/10.1016/j.indcrop.2011.05.012
  31. McClendon, J.H. 1981. The osmotic pressure of concentrated solution of polyethylene glycol 6000, and its variation with temperature. J. Exp. Bot. 32:861-866. https://doi.org/10.1093/jxb/32.4.861
  32. McDonald, M.B. 2000. Seed priming. In Black M and Bewly, J.D. (eds.). Seed technology and its biological basis. CRC Press LLC. Boca Raton, Florida. USA. pp. 287-325.
  33. Mexal, J., Fisher, J.T., Osteryoung, J. and Patrick Reid, C.P. 1975. Oxygen availability in polyethylene glycol solutions and its implication in plant-water relations. Plant Physiol. 55:20-24. https://doi.org/10.1104/pp.55.1.20
  34. Michel, B.E. and Kaufmann, M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51:914-916. https://doi.org/10.1104/pp.51.5.914
  35. Min, B.S. 2002. Treatment techniques for improving a germination of turfgrass seed. M.S. thesis, Yonsei Univ., Seoul, Korea. p. 6. (In Korean)
  36. Ming, L.C. and Kim, D.H. 2000. Agrobactelium mediated transformation of Korean lawngrass (Zoysia japonica). J. Kor. Soc. Hort. Sci. 41:455-458. (In Korean)
  37. SAS Institute Inc. 2003. SAS/STAT Statistical Software. Version 9.1 SAS publishing Cary, NC, USA.
  38. Volterrani, M., Grossi, N. Lulli, F. and Gaetani, M. 2007. Establishment of warm season turfgrass species by transplant of single potted plants. Acta Hort. 783:77-84.
  39. Wada, S. and Reed, B.M. 2011. Optimized scarification protocols improve germination of diverse Rubus germplasm. Sci. Hort. 130:660-664. https://doi.org/10.1016/j.scienta.2011.08.023
  40. Woo, K.J., Yang, G.M. and Choi, J.S. 2004. Effect of 11 preemergence herbicides on vegetative establishment of zoysiagrass (Zoysia japonica). Kor. J. Hort. Sci. Technol. 22:216-222. (In Korean)
  41. Yeam, D.Y., Murray, J.J., Portz, H.L. and Joo, Y.K. 1985. Optimum seed coat scarification and light treatment for the germination of zoysiagrass (Zoysia japonica Steud.) seed. J. Kor. Soc. Hort. Sci. 26:179-185. (In Korean)

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