농약과학회지 (The Korean Journal of Pesticide Science)

  • 제18권3호
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  • Pages.141-147
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  • 2014
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
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생약 중 Fenpyroximate의 분석법 연구

Analytical Methods of Fenpyroximate in Herbal Medicines

  • 이주희 (대구지방식품의약품안전청 유해물질분석과) ;
  • 이윤정 (식품의약품안전평가원 생약연구과) ;
  • 강인호 (식품의약품안전평가원 생약연구과) ;
  • 김도훈 (식품의약품안전평가원 생약연구과) ;
  • 강신정 (식품의약품안전평가원 생약연구과) ;
  • 안경아 (대구지방식품의약품안전청 유해물질분석과) ;
  • 이륜경 (대구지방식품의약품안전청 유해물질분석과) ;
  • 서상철 (대구지방식품의약품안전청 유해물질분석과) ;
  • 이정림 (대구지방식품의약품안전청 유해물질분석과)
  • Lee, Ju-Hee (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Lee, Yoon-Jeong (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, In-Ho (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Do-Hoon (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, Shin-Jeong (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • An, Kyung-A (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Lee, Ryun-Kyung (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Suh, Sang-Chul (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration) ;
  • Lee, Jeong-Rim (Hazardous Substances Analysis Division, Daegu Regional Food & Drug Administration)
  • 투고 : 2014.05.16
  • 심사 : 2014.07.10
  • 발행 : 2014.09.30
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초록

Fenpyroximate는 생약 재배시 살응애제로 사용되는 피라졸계 살충제이다. 본 실험은 HPLC-PDA와 HPLC-MS/MS를 이용하여 생약 중 fenpyroximate의 잔류분석법 확립을 위해 수행하였다. 생약(감초와 홍화) 중 잔류하는 fenpyroximate를 아세톤으로 추출하고, 추출액을 다시 포화식염수와 dichloromethane을 이용하여 액-액 분배한 후 아미노프로필 카트리지와 florisil로 정제한 것을 HPLC-PDA와 HPLC-MS/MS로 정량하였다. 생약별로 fenpyroximate를 2 또는 3 수준으로 첨가하여 수행한 회수율 범위는 72.0~106.4%이었으며 이때의 변이계수는 0.2~4.4였다. 따라서 본 분석법은 생약 중의 fenpyroximate 잔류 분석법으로서의 충분한 재현성과 감도를 나타내었다.

Fenpyroximate is acaricide of pyrazole group. This acaricide have already been permitted for herb cultivation. This experiment was conducted to establish a determination method for fenpyroximate residue in herbal medicines using HPLC-PDA and HPLC-MS/MS. Fenpyroximate residue was extracted with acetone from samples of herbal which Liquorice Root (Glycyrrhiza uralensis) and Safflower Seed (Carthamus tinctorius Linne). The extract was diluted with saturated saline water and dichloromethane liquid-liquid partition (extraction) was followed to recover fenpyroximate from the aqueous phase. Amino propyl ($NH_2$) and florisil column chromatography was additionally employed for final clean up of the extract. The fenpyroximate was quantitated by HPLC-PDA and HPLC-MS/MS. The herbals were fortified with fenpyroximate at 2 or 3 levels per crop. Mean recovery ratio were ranged from 72.0 to 106.4%. The coefficients of variation were ranged from 0.2 to 4.4. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fenpyroximate in herbal medicines.

키워드

참고문헌

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피인용 문헌

  1. Determination of Fenpyroximate in Aqueous Matrices by Dispersive Liquid-Liquid Microextraction vol.41, pp.1, 2020, https://doi.org/10.1002/bkcs.11922
  2. QuEChERS 전처리와 GC-MS/MS를 활용한 생약의 잔류농약 분석 가능성 연구 vol.51, pp.3, 2020, https://doi.org/10.22889/kjp.2020.51.3.207