Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Yam (Dioscorea batatas) Root and Bark Extracts Stimulate Osteoblast Mineralization by Increasing Ca and P Accumulation and Alkaline Phosphatase Activity

  • Kim, Suji (Department of Food and Nutrition, Andong National University) ;
  • Shin, Mee-Young (Department of Food and Nutrition, Andong National University) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University) ;
  • Lim, Jae-Hwan (Department of Biological Sciences, Andong National University) ;
  • Lee, Jong-Hwa (Department of Food Science and Biotechnology, Andong National University) ;
  • Kwun, In-Sook (Department of Food and Nutrition, Andong National University)
  • Received : 2014.07.30
  • Accepted : 2014.09.24
  • Published : 2014.09.30
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Abstract

Yam (Dioscorea batatas) is widely consumed as functional food for health promotion mainly in East Asia countries. We assessed whether yam root (tuber) or bark (peel) extracts stimulated the activity of osteoblasts for osteogenesis. MC3T3-E1 cells (mouse osteoblasts) were treated with yam root extracts (water or methanol) (study I) or bark extracts (water or hexane) (study II) within $0{\sim}10{\mu}g/mL$ during the periods of osteoblast proliferation (5~10 day), matrix maturation (11~15 day) and mineralization (16~20 day) as appropriate. In study I, both yam root water and methanol extracts increased cell proliferation as concentration-dependent manner. Cellular collagen synthesis and alkaline phosphatase (ALP) activity, both the indicators of bone matrix protein and inorganic phosphate production for calcification respectively, were also increased by yam root water and methanol extract. Osteoblast calcification as cell matrix Ca and P accumulation was also increased by the addition of yam root extracts. In study II, yam bark extracts (water and hexane) increased osteoblast proliferation and differentiation, as collagen synthesis and ALP activity and osteoblast matrix Ca and P deposition. The study results suggested that both yam root and bark extracts stimulate osteogenic function in osteoblasts by stimulating bone matrix maturation by increasing collagen synthesis, ALP activity, and matrix mineralization.

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References

  1. Katagiri T, Takahashi N. 2002. Regulatory mechanisms of osteoblast and osteoclast differentiation. Oral Dis 8: 147-159. https://doi.org/10.1034/j.1601-0825.2002.01829.x
  2. Wang D, Christensen K, Chawla K, Xiao G, Krebsbach PH, Franceschi RT. 1999. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 14: 893-903. https://doi.org/10.1359/jbmr.1999.14.6.893
  3. Stein GS, Lian JB, Owen TA. 1990. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J 4: 3111-3123.
  4. Gerstenfeld LC, Riva A, Hodgens K, Eyre DR, Landis WJ. 1993. Post-translational control of collagen fibrillogenesis in mineralizing cultures of chick osteoblasts. J Bone Miner Res 8: 1031-1043.
  5. Sudo H, Kodama HA, Amagai Y, Yamamoto S, Kasai S. 1983. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 96: 191-198. https://doi.org/10.1083/jcb.96.1.191
  6. Quarles LD, Yohay DA, Lever LW, Caton R, Wenstrup RJ. 1992. Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res 7: 683-692.
  7. Kwun IS, Cho YE, Lomeda RA, Shin HI, Choi JY, Kang YH, Beattie JH. 2010. Zinc deficiency suppresses matrix mineralization and retards osteogenesis transiently with catch-up possibly through Runx 2 modulation. Bone 46: 732-741. https://doi.org/10.1016/j.bone.2009.11.003
  8. Cho YE, Alcantara E, Kumaran S, Son KH, Sohn HY, Lee JH, Choi CS, Ha TY, Kwun IS. 2010. Red yeast rice stimulates osteoblast proliferation and increases alkaline phosphatase activity in MC3T3-E1 cells. Nutr Res 30: 501-510. https://doi.org/10.1016/j.nutres.2010.06.011
  9. Yang DJ, Lu TJ, Hwang LS. 2003. Isolation and identification of steroidal saponins in Taiwanese yam cultivar (Dioscorea pseudojaponica Yamamoto). J Agric Food Chem 51: 6438-6444. https://doi.org/10.1021/jf030390j
  10. Chiang CT, Way TD, Tsai SJ, Lin JK. 2007. Diosgenin, a naturally occurring steroid, suppresses fatty acid synthase expression in HER2-overexpressing breast cancer cells through modulating Akt, mTOR and JNK phosphorylation. FEBS Lett 581: 5735-5742. https://doi.org/10.1016/j.febslet.2007.11.021
  11. Huong PT, Lee MY, Lee KY, Chang IY, Lee SK, Yoon SP, Lee DC, Jeon YJ. 2012. Synergistic induction of iNOS by IFN-$\gamma$ and glycoprotein isolated from Dioscorea batatas. Korean J Physiol Pharmacol 16: 431-436. https://doi.org/10.4196/kjpp.2012.16.6.431
  12. Jin M, Lu Y, Yang JH, Jo TH, Park YI, Lee CK, Park SJ, Son KH, Chang HW. 2011. Anti-inflammatory activity of 6-hydroxy-2, 7-dimethoxy-1,4-henanthraquinone from tuberous roots of yam (Dioscorea batatas) through inhibition of prostaglandin $D_2$ and leukotriene $C_4$ production in mouse bone marrowderived mast cells. Arch Pharm Res 34: 1495-1501. https://doi.org/10.1007/s12272-011-0911-8
  13. Jin M, Suh SJ, Yang JH, Lu Y, Kim SJ, Kwon S, Jo TH, Kim JW, Park YI, Ahn GW, Lee CK, Kim CH, Son JK, Son KH, Chang HW. 2010. Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-$\kappa{B}$ and ERK1/2 inactivation. Food Chem Toxicol 48: 3073-3079. https://doi.org/10.1016/j.fct.2010.07.048
  14. Yeh YH, Hsieh YL, Lee YT. 2013. Effects of yam peel extract against carbon tetrachloride-induced hepatotoxicity in rats. J Agric Food Chem 61: 7387-7396. https://doi.org/10.1021/jf401864y
  15. Kim SY, Jwa HJ, Yanagawa Y, Park TS. 2012. Extract from Dioscorea batatas ameliorates insulin resistance in mice fed a high-fat diet. J Med Food 15: 527-534. https://doi.org/10.1089/jmf.2011.2008
  16. Son IS, Kim JH, Sohn HY, Son KH, Kim JS, Kwon CS. 2007. Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp.), on high-cholesterol fed rats. Biosci Biotechnol Biochem 71: 3063-3071. https://doi.org/10.1271/bbb.70472
  17. Yen ML, Su JL, Chien CL, Tseng KW, Yang CY, Chen WF, Chang CC, Kuo ML. 2005. Diosgenin induces hypoxiainducible factor-1 activation and angiogenesis through estrogen receptor-related phosphatidylinositol 3-kinase/Akt and p38 mitogen-activated protein kinase pathways in osteoblasts. Mol Pharmacol 68: 1061-1073. https://doi.org/10.1124/mol.104.010082
  18. Higdon K, Scott A, Tucci M, Benghuzzi H, Tsao A, Puckett A, Cason Z, Hughes J. 2001. The use of estrogen, DHEA, and diosgenin in a sustained delivery setting as a novel treatment approach for osteoporosis in the ovariectomized adult rat model. Biomed Sci Instrum 37: 281-286.
  19. Alcantara EH, Shin MY, Sohn HY, Park YM, Kim T, Lim JH, Jeong HJ, Kwon ST, Kwun IS. 2011. Diosgenin stimulates osteogenic activity by increasing bone matrix protein synthesis and bone-specific transcription factor Runx2 in osteoblastic MC3T3-E1 cells. J Nutr Biochem 22: 1055-1063. https://doi.org/10.1016/j.jnutbio.2010.09.003
  20. Shin MY, Alcantara EH, Park YM, Kwon ST, Kwun IS. 2011. Yam extracts increase cell proliferation and bone matrix protein collagen synthesis of murine osteoblastic MC3T3-E1 cells. J Food Sci Nutr 16: 291-298. https://doi.org/10.3746/jfn.2011.16.4.291
  21. Bessey OA, Love RH. 1952. Preparation and measurement of the purity of the phosphatase reagent, disodium p-nitrophenyl phosphate. J Biol Chem 196: 175-178.
  22. Koeneman KS, Yeung F, Chung LW. 1999. Osteomimetic properties of prostate cancer cells: a hypothesis supporting the predilection of prostate cancer metastasis and growth in the bone environment. Prostate 39: 246-261. https://doi.org/10.1002/(SICI)1097-0045(19990601)39:4<246::AID-PROS5>3.0.CO;2-U
  23. Franceschi RT, Iyer BS. 1992. Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells. J Bone Miner Res 7: 235-246.
  24. Franceschi RT, Iyer BS, Cui Y. 1994. Effects of ascorbic acid on collagen matrix formation and osteoblast differentiation in murine MC3T3-E1 cells. J Bone Miner Res 9: 843-854.
  25. Withold W, Schulte U, Reinauer H. 1996. Method for determination of bone alkaline phosphatase activity: analytical performance and clinical usefulness in patients with metabolic and malignant bone diseases. Clin Chem 42: 210-217.
  26. Beck GR Jr, Sullivan EC, Moran E, Zerler B. 1998. Relationship between alkaline phosphatase levels, osteopontin expression, and mineralization in differentiating MC3T3-E1 osteoblasts. J Cell Biochem 68: 269-280. https://doi.org/10.1002/(SICI)1097-4644(19980201)68:2<269::AID-JCB13>3.0.CO;2-A
  27. Marsh ME, Munne AM, Vogel JJ, Cui Y, Franceschi RT. 1995. Mineralization of bone-like extracellular matrix in the absence of functional osteoblasts. J Bone Miner Res 10: 1635-1643.
  28. Murshed M, Harmey D, Millan JL, McKee MD, Karsenty G. 2005. Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone. Genes Dev 19: 1093-1104. https://doi.org/10.1101/gad.1276205

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