Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Classification and Antioxidant Activities of Mangrove Plants in Weno Island, Micronesia

미크로네시아 웨노섬 서식 망그로브 식물의 분류 및 항산화 활성

  • Chung, Youngjae (Department of Life Science and Biotechnology, Shin Gyeong University) ;
  • Hwang, Jinik (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology) ;
  • Suh, Sung-Suk (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology) ;
  • Park, Mirye (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology) ;
  • Kim, Donggiun (Department of Biological Science, Silla University) ;
  • Park, Jongbum (Department of Biological Science, Silla University) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science and Technology)
  • 정영재 (신경대학교 생명공학과) ;
  • 황진익 (한국해양과학기술원 남해특성연구부) ;
  • 서승석 (한국해양과학기술원 남해특성연구부) ;
  • 박미례 (한국해양과학기술원 남해특성연구부) ;
  • 김동균 (신라대학교 생명과학과) ;
  • 박종범 (신라대학교 생명과학과) ;
  • 이택견 (한국해양과학기술원 남해특성연구부)
  • Received : 2014.08.11
  • Accepted : 2014.09.11
  • Published : 2014.09.30
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Abstract

Mangrove plants serve as a sink of heavy metals and contain phenolic compounds at a high level. Therefore, with mangrove plants, recent studies to develop phytoremediation and natural antioxidants have been conducted in the commercial and academic fields. In the present study, six mangroves in Weno Island of Micronesia were investigated for their phylogenetic relationship and antioxidant activities. First, to determine the phylogenetic relationship among them, rbcL (large subunit of ribulose-bisphosphate carboxylase), one of the chloroplast genes, was used as a molecular marker. According to the data, Xylocarpus, Sonneratia and Rhizophora showed close similarity but not Excoecaria. The levels of phenolic compounds in the bark were abundant in R. apiculata and X. granatum, accounting for 1.10 mM/mg, while R. stylosa and S. alba contained the low amounts, representing 0.73 mM/mg and 0.72 mM/mg, respectively (p<0.05). In addition, bark extracts from R. apiculata, X. granatum, X. moluccensis, and E. agallocha had high antioxidant activities through the DPPH radical scavenging activity and ABTS analysis, whereas S. alba showed the lowest activities. These results suggest that the bark of R. apiculata can be used as a good source for the development of natural antioxidants.

망그로브 식물은 중금속의 sink로 작용하며, 페놀성 화합물의 함량이 높은 것으로 알려져 있다. 따라서 최근에 망그로브 식물을 이용한 phytoremediation과 천연항산화제 개발을 위한 연구가 활발히 수행되고 있다. 이 연구에서는 미크로네시아 웨노섬에 서식하고 있는 4속 6종의 망그로브 식물에 대하여 분류 및 각 종의 잎에서의 항산화 활성을 분석 비교하였다. 6종의 계통관계를 조사하기 위한 분자마커로 엽록체 유전자인 rbcL (large subunit of ribulose-bisphosphate carboxylase)이 사용되었다. 그 결과 Xylocarpus, Sonneratia, Rhizophora 속 순으로 계통적 유사도가 높았으며 Excoecaria 속이 가장 유사도가 낮았다. 한편 6종의 망그로브 줄기 껍질의 페놀성 화합물 함량은 R. apiculata와 X. granatum에서 가장 높게 나타났으며 (1.10 mM/mg), R. stylosa (0.73 mM/mg)와 S. alba (0.72 mM/mg)에서 가장 낮았다(p<0.05). 또한 DPPH 와 ABTS 방법을 이용한 분석 결과, R. apiculate, X. granatum, X. moluccensis 및 E. agallocha는 높은 항산화 활성을 보인 반면, S. alba는 가장 낮은 활성을 보였다. 이러한 결과는 R. apiculata의 줄기껍질이 천연 항산화제 개발을 위한 좋은 원료가 될 수 있음을 의미한다.

Keywords

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