Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Anti-inflammatory Effects of Korean red ginseng Extract in formalin-induced Orofacial Pain in Rats

포르말린으로 유도된 안면통증에 대한 홍삼추출물의 항염증 효과

  • Received : 2014.07.23
  • Accepted : 2014.09.11
  • Published : 2014.09.30
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Abstract

The effects of korean red ginseng (KRG) extracts on orifacial pain control in terms of the systemic inflammatory response and pharmacological effects as health supplements were investigated. The experimental group were divided into three groups, the control group (n=6), formalin (5%, $50{\mu}{\ell}$) injection group (n=6), and formalin (5%, $50{\mu}{\ell}$) injection added KRG administrated group (4.5 ml/kg, n=6). The KRG administrated group prior to the formalin injection significantly attenuated the behavioral response compared to that of the control group. Pain reduction was suppressed mainly from 15 min to 30 min. The KRG administrated rats showed significantly reduced p38 MAPK, iNOS and Nrf2 expression in the brain and medulla oblongata according to Western blot analysis. These findings suggest that KRE may have a useful effect on orificial pain control functions by preventing the p38 MAPK pathway.

본 연구는 건강보조제 및 약제로서 전신의 염증반응 및 여러 약리적 효능을 가지고 있는 홍삼이 국소적인 안면 통증의 완화에 영향을 끼치는지에 관해 조사하였다. 실험동물은 4그룹으로 대조군(n=6)과 5% 포르말린 주입군(n=6), 5% 포르말린+D.W. 주입군, 홍삼(4.5 ml)(n=6) 복용군으로 나누어 45분간 안면을 긁거나 문지르는 행위를 계수하여 비교하였다. 홍삼 복용군에서 포르말린 주입군에 비해 유의하게 긁거나 문지르는 행위가 줄어들었으며, 15분부터 통증완화효과가 증가되기 시작되어 30분까지 유의한 효과를 보였다. 홍삼투여군에 대한 단백질정량분석 (western blot)으로 통증유발기전에 연관되는 염증과 항산화, NO생성에 관련된 신호전달 단백질 p38 MAPK와 iNOS, Nrf2를 뇌, 연수에서 활성정도를 비교한 결과 감소하는 경향을 확인할 수 있었다. 이상의 결과들은 홍삼이 전신적 약리효과 뿐만 아니라 국소적인 염증, 통증완화에도 효능이 있음 나타낸다.

Keywords

References

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